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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS

Full text
Author(s):
Mokochinski, Joao Benhur [1] ; Bataglion, Giovana Anceski [2] ; Kiyota, Eduardo [1] ; de Souza, Leticia Marrone [1] ; Mazzafera, Paulo [1] ; Helena Frankland Sawaya, Alexandra Christine [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, UNICAMP, Dept Plant Biol, Inst Biol, BR-13083970 Campinas, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Inst Chem, ThoMSon Mass Spectrometry Lab, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ANALYTICAL AND BIOANALYTICAL CHEMISTRY; v. 407, n. 23, p. 7221-7227, SEP 2015.
Web of Science Citations: 6
Abstract

A simple extraction protocol and an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) method for the determination of the syringyl/guaiacyl (S/G) ratio in lignin is reported herein. The method was entirely developed using stems of three Eucalyptus species, which were hydrolyzed with NaOH and partitioned with ethyl ether; vanillin (from the G monomer) and syringaldehyde (from S monomer) were quantified. The S/G ratios obtained were comparable to those usually reported for eucalyptus. The data for one of the eucalyptus species were compared with those obtained with a widely accepted method using thioacidolysis and gas chromatography-mass spectrometry (GC-MS). The method was also applied to sugarcane and showed to be reliable. The yield of the NaOH hydrolysis of the monolignols ranged from 89.94 to 95.69 %, with more than 77.12 % of recuperation in the liquid-liquid extraction. The whole analytical procedure was validated, achieving results with less than 4.38 % of variation. The lowest LOD and LOQ were 0.01 and 0.05 mu g/mL, respectively. In addition, the method combines reliability and a fast and direct quantification. (AU)

FAPESP's process: 11/51949-5 - Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses
Grantee:Paulo Mazzafera
Support type: Research Grants - Research Partnership for Technological Innovation - PITE
FAPESP's process: 13/21306-0 - DEVELOPMENT OF ANALYTICAL METHODS BY UPLC-MS FOR THE METABOLOMIC STUDY OF TWO SPECIES OF EUCALYPTUS EXPOSED TO CHANGES IN TEMPERATURE AND CONCENTRATION OF CO2
Grantee:João Benhur Mokochinski
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Grantee:Paulo Mazzafera
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
FAPESP's process: 12/21395-0 - Molecular characterization of sedimentary organic matter in the Estuary-Lagoon System Cananéia-Iguape by Fourier transform ion cyclotron resonance mass spectrometry
Grantee:Giovana Anceski Bataglion
Support type: Scholarships in Brazil - Doctorate