Physiological responses of Eucalyptus globulus and E. grandis to high concentration of CO2 and temperature variations, identified by metabolomic and transcriptomic analyses

• Grant number: 11/51949-5
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE
• Field of knowledge: Biological Sciences - Botany - Pant Physiology
• Agreement: Agilent
• Principal Investigator: Paulo Mazzafera
• Grantee: Paulo Mazzafera
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Company: Agilent Technologies Brasil Ltda
• City: Campinas
• Associated scholarship(s): 14/17123-0 - Optimizing extraction conditions for metabolomic study of eucalyptus, BP.TT

Abstract

Perennial plant species are constantly challenged by changes in their environment and to survive these unstable conditions, they developed anatomical, cellular and molecular mechanisms. Extremes of temperature are among the stresses that limit the geographical distribution and seasonal growth of various plants, affecting the quality and productivity in crops and forest plantations. However, climate models have indicated not only changes in global temperature, but also the amount of carbon dioxide (CO2) in the atmosphere. Several studies have shown that the cultivation of woody plants in CO2-rich atmosphere results in higher increases of biomass. However, growth under this condition is less pronounced when individuals are subjected to natural field conditions where there is competition for other resources, indicating the existence of a complex network of interactions between environmental factors and atmospheric CO2 concentration. Cellulose and lignin are the main polymers in plants. Eucalyptus is the largest source of cellulose in the world and lignin is a problem in the process of paper making, although it plays an important role in plants providing the support for cellulose micro fibrils in the cell walls. The eucalyptus is the forest crop of greater significance in Brazil, the world's largest producer of cellulose, and the cellulose industry is responsible for generating over 150 thousand jobs in the country. The objective of this proposal is to study the combined effect [low and high] temperature variation and increased levels of CO2 in the atmosphere has on the species Eucalyptus grandis and E. globulus. The second species grows better at lower temperatures and have impaired growth at higher temperatures. Its wood is of excellent quality, presenting high-yield pulp. Transcriptomic and metabolomics are proposed to assess this interaction. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOKOCHINSKI, JOAO BENHUR; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; MUMM, ROLAND; HENDRICUS DE VOS, RIC CORNELIS; HALL, ROBERT DAVID. Metabolic responses of Eucalyptus species to different temperature regimes. JOURNAL OF INTEGRATIVE PLANT BIOLOGY, v. 60, n. 5, p. 397-411, MAY 2018. (08/58035-6, 11/51949-5, 13/21306-0, 15/06987-7)

LEONARDI, GABRIELA DE ALMEIDA; CARLOS, NATALIA APARECIDA; MAZZAFERA, PAULO; BALBUENA, TIAGO SANTANA. Eucalyptus urograndis stem proteome is responsive to short-term cold stress. GENETICS AND MOLECULAR BIOLOGY, v. 38, n. 2, p. 191-198, 2015. (11/23582-0, 11/11650-0, 11/51949-5)

FELTRIM, DANIELA; PEREIRA, LUCIANO; DE SANTANA COSTA, MARILIA GABRIELA; BALBUENA, TIAGO SANTANA; MAZZAFERA, PAULO. Stem aquaporins and surfactant-related genes are differentially expressed in two Eucalyptus species in response to water stress. PLANT STRESS, v. 1, JAN 2021. (11/51949-5, 11/11650-0)

MOKOCHINSKI, JOAO BENHUR; BATAGLION, GIOVANA ANCESKI; KIYOTA, EDUARDO; DE SOUZA, LETICIA MARRONE; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 407, n. 23, p. 7221-7227, SEP 2015. (08/58035-6, 11/51949-5, 13/21306-0, 12/21395-0)

TOLENTINO, FELIPE THADEU; VASCONCELOS, ADRIELLE AYUMI; SOUZA, UIARA ROMERO; GUIMARAES PEREIRA, GONCALO AMARANTE; CARAZOLLE, MARCELO FALSARELLA; MAZZAFERA, PAULO. Identification of microRNAs and their expression profiles on tension and opposite wood of Eucalyptus. THEORETICAL AND EXPERIMENTAL PLANT PHYSIOLOGY, v. 34, n. 4, p. 16-pg., 2022-09-22. (14/23230-4, 11/51949-5)

FELTRIM, DANIELA; GUPTA, BANDANA; GUNDIMEDA, SEETARAMANJANEYULU; KIYOTA, EDUARDO; DOMINGUES JUNIOR, ADILSON PEREIRA; CINTRA, LEANDRO CARRIJO; MAZZAFERA, PAULO. xposure of Eucalyptus to varied temperature and CO2 has a profound effect on the physiology and expression of genes related to cell wall formation and remodelin. Tree Genetics & Genomes, v. 18, n. 1, FEB 2022. (11/51949-5)