Changes in the protein expression profile of eucalyptus globulus in response to variations in the growth temperature and atmospheric carbon dioxide concentration

• Grant number: 11/11650-0
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: January 01, 2012 - December 31, 2015
• Field of knowledge: Agronomical Sciences - Forestry Resources and Forestry Engineering - Forestry
• Principal Investigator: Tiago Santana Balbuena
• Grantee: Tiago Santana Balbuena
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Paulo Mazzafera
• Associated grant(s): 18/15035-8 - Probing Eucalytpus plant performance to atmospheric carbon dioxide levels: the source/sink relationships unveiled by targeted proteomics approach, AP.JP2
• Associated scholarship(s): 11/23582-0 - Changes in the protein expression profile of eucalyptus globulus in response to variations in the growth temperature and atmospheric carbon dioxide concentration, BP.JP

Abstract

Eucalyptus globulus wood has become highly attractive to the paper and pulp industry due to its high-yield conversion into cellulose. However, environmental factors can limit plant growth and affect cellulose yield. The physiological effects induced by different growth conditions have been studied in E. globulus; nevertheless, the molecular responses to these variations have not yet been completely elucidated. The present proposal aims the characterization of the changes in the protein expression profile, as the final products of gene expression, induced by different growth temperatures and carbon dioxide concentrations (CO2) and correlate the observed changes with growth and lignin accumulation in the species. For that, two modules are proposed. In the first module, growth and proteome changes induced by different temperatures will be evaluated and the results obtained will be compared to those observed for E. grandis, whose wood is currently the mostly used feedstock in the paper and pulp industry. In the second module, lignin composition and proteome changes induced by different CO2 atmospheric concentrations (360 and 720 ppm) will be evaluated under different growth temperatures that will be further determined according to the results obtained in the first module. The proposal aims to contribute to a better understanding of the effects of the environmental factors studied here on the growth and wood chemical composition of E. globulus. The identification of differentially expressed proteins will also assist on the development of selection markers for plants with higher temperature tolerance and optimized growth. Eventually, the information obtained here may assist genetic transformation of other eucalyptus species aiming to increase the cellulose yield. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHICONATO, DENISE A.; DE SANTANA COSTA, MARILIA G.; BALBUENA, TIAGO S.; MUNNS, RANA; DOS SANTOS, DURVALINA M. M.. Proteomic analysis of young sugarcane plants with contrasting salt tolerance. FUNCTIONAL PLANT BIOLOGY, v. 48, n. 6, p. 588-596, 2021. (11/11650-0)

LEONARDI, GABRIELA DE ALMEIDA; CARLOS, NATALIA APARECIDA; MAZZAFERA, PAULO; BALBUENA, TIAGO SANTANA. Eucalyptus urograndis stem proteome is responsive to short-term cold stress. GENETICS AND MOLECULAR BIOLOGY, v. 38, n. 2, p. 191-198, 2015. (11/23582-0, 11/11650-0, 11/51949-5)

FELTRIM, DANIELA; PEREIRA, LUCIANO; DE SANTANA COSTA, MARILIA GABRIELA; BALBUENA, TIAGO SANTANA; MAZZAFERA, PAULO. Stem aquaporins and surfactant-related genes are differentially expressed in two Eucalyptus species in response to water stress. PLANT STRESS, v. 1, JAN 2021. (11/51949-5, 11/11650-0)

CORREA DE SOUZA, RAFAEL SOARES; BALBUENA, TIAGO SANTANA; ARRUDA, PAULO. Structure, Organization, and Expression of the Alpha Prolamin Multigenic Family Bring New Insights into the Evolutionary Relationships among Grasses. PLANT GENOME, v. 8, n. 1, MAR 2015. (11/11650-0, 11/12431-0)

DOS SANTOS, BRUNA MARQUES; BALBUENA, TIAGO SANTANA. Carbon assimilation in Eucalyptus urophylla grown under high atmospheric CO2 concentrations: A proteomics perspective. JOURNAL OF PROTEOMICS, v. 150, p. 252-257, JAN 6 2017. (11/11650-0, 14/07454-0)

SAMPAIO, PAULO HENRIQUE; FIDELIS JUNIOR, OTAYIO LUIZ; MARQUES, LUIZ CARLOS; MACHADO, ROSANGELA ZACARIAS; BARNABE, PATRICIA DE ATHAYDE; ANDRE, MARCOS ROGERIO; BALBUENA, TIAGO SANTANA; CADIOLI, FABIANO ANTONIO. Acute-phase protein behavior in dairy cattle herd naturally infected with Trypanosoma vivax. Veterinary Parasitology, v. 211, n. 3-4, p. 141-145, JUL 30 2015. (11/03302-2, 11/11650-0, 11/21531-9)

DE SANTANA COSTA, MARILIA GABRIELA; MAZZAFERA, PAULO; BALBUENA, TIAGO SANTANA. Insights into temperature modulation of the Eucalyptus globulus and Eucalyptus grandis antioxidant and lignification subproteomes. Phytochemistry, v. 137, p. 15-23, MAY 2017. (11/11650-0, 14/23541-0)

DE SANTANA COSTA, MARILIA GABRIELA; FELTRIM, DANIELA; MAZZAFERA, PAULO; BALBUENA, TIAGO SANTANA. Revisiting the stem proteome of Eucalyptus grandis and Eucalyptus globulus: Identification of temperature-induced changes. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1868, n. 12, p. 4-pg., 2020-12-01. (14/23541-0, 11/11650-0)