DEVELOPMENT OF ANALYTICAL METHODS BY UPLC-MS FOR THE METABOLOMIC STUDY OF TWO SPECIES OF EUCALYPTUS EXPOSED TO CHANGES IN TEMPERATURE AND CONCENTRATION OF CO2

• Grant number: 13/21306-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: May 01, 2014
• End date: February 28, 2017
• Field of knowledge: Agronomical Sciences - Agronomy
• Principal Investigator: Alexandra Christine Helena Frankland Sawaya
• Grantee: João Benhur Mokochinski
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 15/06987-7 - Untargeted metabolomics of eucalyptus: from data acquisition to biological interpretation, BE.EP.DR

Abstract

Studies indicate that changes in temperature and CO2 concentrations influence the growth of different plant species, forcing them to develop complex mechanisms for adaptation to survive. Brazil is the largest producer of cellulose, and the main source for obtaining this raw material is eucalyptus (E. grandis and E. globulus), which justifies studies to understand how these species create mechanisms for survival in adverse conditions of temperature and CO2 concentration. The metabolomic analysis aims to identify and quantify the metabolites produced by organisms, being a useful tool in the elucidation of the end products of a biological system. The ultra-efficiency liquid chromatography coupled to mass spectrometry (UPLC-MS) has been shown to be a robust technique for analysis of metabolites, since it allows a high degree of reproducibility, small volume of solvents and shorter analysis time. However, one of the difficulties in the identification of these metabolites is that there is not a single method of extraction and analysis for all classes of compounds. The objective of this work is to develop and validate analytical methods for UPLC-MS to identify and quantify metabolites of eucalyptus species (E. grandis and E. globulus), and employ the factorial design optimization of extraction conditions in leaves and stem, in order to utilize a small number of extracts to evaluate the different classes of compounds.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIEIRA, RONIERIK PIOLI; MOKOCHINSKI, JOAO BENHUR; SAWAYA, ALEXANDRA C. H. F.. MATHEMATICAL MODELING OF ASCORBIC ACID THERMAL DEGRADATION IN ORANGE JUICE DURING INDUSTRIAL PASTEURIZATIONS. Journal of Food Process Engineering, v. 39, n. 6, p. 683-691, DEC 2016. (13/21306-0)

MACHADO, CHRISTIANE SCHINEIDER; MOKOCHINSKI, JOAO BENHUR; DE LIRA, TATIANA ONOFRE; EVANGELISTA DE OLIVEIRA, FATIMA DE CASSIA; CARDOSO, MAGDA VIEIRA; FERREIRA, ROSEANE GUIMARAES; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; FERREIRA, ANTONIO GILBERTO; PESSOA, CLAUDIA; CUESTA-RUBIO, OSMANY; et al. Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis. Evidence-based Complementary and Alternative Medicine, 2016. (13/21306-0)

MOKOCHINSKI, JOAO BENHUR; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; MUMM, ROLAND; HENDRICUS DE VOS, RIC CORNELIS; HALL, ROBERT DAVID. Metabolic responses of Eucalyptus species to different temperature regimes. JOURNAL OF INTEGRATIVE PLANT BIOLOGY, v. 60, n. 5, p. 397-411, MAY 2018. (08/58035-6, 11/51949-5, 13/21306-0, 15/06987-7)

MOKOCHINSKI, JOAO BENHUR; BATAGLION, GIOVANA ANCESKI; KIYOTA, EDUARDO; DE SOUZA, LETICIA MARRONE; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 407, n. 23, p. 7221-7227, SEP 2015. (08/58035-6, 11/51949-5, 13/21306-0, 12/21395-0)

VIEIRA, RONIERIK PIOLI; MOKOCHINSKI, JOAO BENHUR; SAWAYA, ALEXANDRA C. H. F.. MATHEMATICAL MODELING OF ASCORBIC ACID THERMAL DEGRADATION IN ORANGE JUICE DURING INDUSTRIAL PASTEURIZATIONS. JOURNAL OF FOOD PROCESS ENGINEERING, v. 39, n. 6, p. 9-pg., 2016-12-01. (13/21306-0)

MACHADO, CHRISTIANE SCHINEIDER; MOKOCHINSKI, JOAO BENHUR; DE LIRA, TATIANA ONOFRE; EVANGELISTA DE OLIVEIRA, FATIMA DE CASSIA; CARDOSO, MAGDA VIEIRA; FERREIRA, ROSEANE GUIMARAES; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; FERREIRA, ANTONIO GILBERTO; PESSOA, CLAUDIA; CUESTA-RUBIO, OSMANY; et al. Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis. Evidence-based Complementary and Alternative Medicine, v. 2016, p. 11-pg., 2016-01-01. (13/21306-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MOKOCHINSKI, João Benhur. Estudo de duas espécies de Eucalipto usando estratégias metabolômicas untargeted e targeted. 2017. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.