| Grant number: | 13/21306-0 |
| Support type: | Scholarships in Brazil - Doctorate |
| Effective date (Start): | May 01, 2014 |
| Effective date (End): | February 28, 2017 |
| Field of knowledge: | Agronomical Sciences - Agronomy |
| Principal Investigator: | Alexandra Christine Helena Frankland Sawaya |
| Grantee: | João Benhur Mokochinski |
| Home Institution: | Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil |
| Associated scholarship(s): | 15/06987-7 - Untargeted metabolomics of eucalyptus: from data acquisition to biological interpretation, BE.EP.DR |
Abstract Studies indicate that changes in temperature and CO2 concentrations influence the growth of different plant species, forcing them to develop complex mechanisms for adaptation to survive. Brazil is the largest producer of cellulose, and the main source for obtaining this raw material is eucalyptus (E. grandis and E. globulus), which justifies studies to understand how these species create mechanisms for survival in adverse conditions of temperature and CO2 concentration. The metabolomic analysis aims to identify and quantify the metabolites produced by organisms, being a useful tool in the elucidation of the end products of a biological system. The ultra-efficiency liquid chromatography coupled to mass spectrometry (UPLC-MS) has been shown to be a robust technique for analysis of metabolites, since it allows a high degree of reproducibility, small volume of solvents and shorter analysis time. However, one of the difficulties in the identification of these metabolites is that there is not a single method of extraction and analysis for all classes of compounds. The objective of this work is to develop and validate analytical methods for UPLC-MS to identify and quantify metabolites of eucalyptus species (E. grandis and E. globulus), and employ the factorial design optimization of extraction conditions in leaves and stem, in order to utilize a small number of extracts to evaluate the different classes of compounds. | |