Control of lignin biosynthesis in sugar cane: many gaps still to be filled

• Grant number: 08/58035-6
• Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• Duration: September 01, 2009 - August 31, 2014
• Field of knowledge: Agronomical Sciences - Agronomy
• Cooperation agreement: CNPq - Pronex
• Principal Investigator: Paulo Mazzafera
• Grantee: Paulo Mazzafera
• Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated grant(s): 13/50315-8 - Identification of target genes regulated by the transcriptions factor shine in monocot plants using chilp-seq, AP.R
• Associated scholarship(s): 12/23187-6 - Lignin metabolism in four sugarcane genotypes grown in field conditions, BP.MS; 12/08471-0 - Analysis of gene expression profile for enzymes of the lignin biosynthesis pathway and dirigent proteins in the species of Saccharum genus, BP.IC; 11/17081-8 - Biosynthesis of lignin in sugarcane under drought and cold stresses, BP.IC; 10/11476-8 - Identification and validation of transcription factors involved with lignin biosynthesis in sugar cane, BP.PD; 10/08232-0 - Control of lignin biosynthesis in sugar cane: dirigent proteins gene family, BP.PD

Abstract

Lignin content may vary in response to several biotic and abiotic stresses and understanding how this occurs may help to understand how to control the lignin biosynthesis and consequently its content in plants. Very little is known about lignin metabolism in sugarcane. However, taking in account the information accumulated for other plants and the agronomical practices and problems in sugarcane cultivation, we may have enough hints to plan several studies on how sugarcane modulates lignin composition and content. Therefore, the aim of this project is 1) to cultivate contrasting sugarcane genetic material for lignin content in six locations well characterized for temperature, water availability and irradiance and analyze lignin, sucrose and cellulose, and then, based on these results to study gene expression and perform a more detailed study of lignin composition; 2) to search the SUCEST database for ESTs coding transcription factors known to be involved in lignin metabolism in model plants and use this information in controlled studies (on water supply, nitrogen fertilization, light intensity and low temperatures under field and greenhouse conditions, and growth chamber) to establish correlations between transcription factors regulation and lignin content and composition; 3) search the SUCEST database for ESTs coding ortologs to peroxidases and laccases and use this information in the controlled studies to evaluate the involvement of these enzymes in lignin biosynthesis; 4) to perform a system biology study of regulatory network involved in lignin biosynthesis. To reach these aims we will use a population segregating for lignin content. With this information we may get some valuable knowledge on the lignin biosynthesis in the complex sugarcane genome. Shortly we believe to have at least initial information on how environmental factors affect different sugarcane genotypes. Also it is expected that our results allow us to understand how genes of the lignin biosynthesis pathway and related transcription factors changes lignin content and structure in sugarcane as influenced by some specific environmental factors. In a long-term objective, we may have the possibility to gain knowledge on the agronomical practices may contribute to decrease lignin in sugarcane. This knowledge on the interaction of environmental factors and control of the lignin biosynthesis and related transcription factors may allows us to design a transgenic plants altered in specific genes, aiming not only the decrease of lignin but also its structure. (AU)

Scientific publications

(34)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALLEVATO, DANIELLA M.; GROPPO, MILTON; KIYOTA, EDUARDO; MAZZAFERA, PAULO; NIXON, KEVIN C. Evolution of phytochemical diversity in Pilocarpus (Rutaceae). Phytochemistry, v. 163, p. 132-146, JUL 2019. Web of Science Citations: 0.

SALVATO, FERNANDO; LOZIUK, PHILIP; KIYOTA, EDUARDO; DANELUZZI, GABRIEL SILVA; ARAUJO, PEDRO; MUDDIMAN, DAVID C.; MAZZAFERA, PAULO. Label-Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress. PROTEOMICS, v. 19, n. 14 JUL 2019. Web of Science Citations: 1.

PORTILLA LLERENA, JUAN PABLO; FIGUEIREDO, RAQUEL; BRITON, MICHAEL DOS SANTOS; KIYOTA, EDUARDO; SAMPAIO MAYER, JULIANA LISCHKA; ARAUJO, PEDRO; SCHIMPL, FLAVIA CAMILA; DAMA, MURALI; PAULY, MARKUS; MAZZAFERA, PAULO. Deposition of lignin in four species of Saccharum. SCIENTIFIC REPORTS, v. 9, APR 10 2019. Web of Science Citations: 0.

MOKOCHINSKI, JOAO BENHUR; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; MUMM, ROLAND; HENDRICUS DE VOS, RIC CORNELIS; HALL, ROBERT DAVID. Metabolic responses of Eucalyptus species to different temperature regimes. JOURNAL OF INTEGRATIVE PLANT BIOLOGY, v. 60, n. 5, p. 397-411, MAY 2018. Web of Science Citations: 9.

ALMEIDA, CLAUDIA DE LAZZARI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; LOPEZ DE ANDRADE, SARA ADRIAN. Mycorrhizal influence on the growth and bioactive compounds composition of two medicinal plants: Mikania glomerata Spreng. and Mikania laevigata Sch Bip. ex Baker (Asteraceae). BRAZILIAN JOURNAL OF BOTANY, v. 41, n. 1, p. 233-240, MAR 2018. Web of Science Citations: 0.

JUAN P.P. LLERENA; PEDRO ARAÚJO; PAULO MAZZAFERA. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum. Anais da Academia Brasileira de Ciências, v. 90, n. 1, p. -, Mar. 2018.

LLERENA, JUAN P. P.; ARAUJO, PEDRO; MAZZAFERA, PAULO. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum. Anais da Academia Brasileira de Ciências, v. 90, n. 1, p. 509-519, MAR 2018. Web of Science Citations: 2.

DE OLIVEIRA COSTA, VANESSA CRUZ; BORGHI, ALEXANDRE AUGUSTO; SAMPAIO MAYER, JULIANA LISCHKA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Comparison of the Morphology, Anatomy, and Chemical Profile of Mikania glomerata and Mikania laevigata. Planta Medica, v. 84, n. 3, p. 191-200, FEB 2018. Web of Science Citations: 2.

FERREIRA, FERNANDA S.; SAMPAIO, GENI R.; KELLER, LAURA M.; SAWAYA, ALEXANDRA C. H. F.; CHAVEZ, DAVY W. H.; TORRES, ELIZABETH A. F. S.; SALDANHA, TATIANA. Impact of Air Frying on Cholesterol and Fatty Acids Oxidation in Sardines: Protective Effects of Aromatic Herbs. Journal of Food Science, v. 82, n. 12, p. 2823-2831, DEC 2017. Web of Science Citations: 4.

NOBILE, PAULA MACEDO; BOTTCHER, ALEXANDRA; MAYER, JULIANA L. S.; BRITO, MICHAEL S.; DOS ANJOS, IVAN A.; DE ANDRADE LANDELL, MARCOS GUIMARAES; VICENTINI, RENATO; CRESTE, SILVANA; RIANO-PACHON, DIEGO MAURICIO; MAZZAFERA, PAULO. Identification, classification and transcriptional profiles of dirigent domain-containing proteins in sugarcane. Molecular Genetics and Genomics, v. 292, n. 6, p. 1323-1340, DEC 2017. Web of Science Citations: 2.

SALVATO, FERNANDA; WILSON, RASHAUN; PORTILLA LLERENA, JUAN PABLO; KIYOTA, EDUARDO; REIS, KARINA LIMA; BOARETTO, LUIS FELIPE; BALBUENA, TIAGO S.; AZEVEDO, RICARDO A.; THELEN, JAY J.; MAZZAFERA, PAULO. Luxurious Nitrogen Fertilization of Two Sugar Cane Genotypes Contrasting for Lignin Composition Causes Changes in the Stem Proteome Related to Carbon, Nitrogen, and Oxidant Metabolism but Does Not Alter Lignin Content. JOURNAL OF PROTEOME RESEARCH, v. 16, n. 10, p. 3688-3703, OCT 2017. Web of Science Citations: 2.

ALMEIDA, CLAUDIA DE LAZZARI; XAVIER, RENATA MOURA; BORGHI, ALEXANDRE AUGUSTO; DOS SANTOS, VIVIAN FRANCESCHINI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Effect of seasonality and growth conditions on the content of coumarin, chlorogenic acid and dicaffeoylquinic acids in Mikania laevigata Schultz and Mikania glomerata Sprengel (Asteraceae) by UHPLC-MS/MS. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, n. SI, p. 162-172, JUL 2017. Web of Science Citations: 8.

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CARNEIRO, M. J.; LOPEZ, B. G-C; LANCELLOTTI, M.; FRANCHI, G. C.; NOWILL, ALEXANDRE E.; SAWAYA, A. C. H. F. Evaluation of the chemical composition and biological activity of extracts of Tetragonisca angustula propolis and Schinus terebinthifolius Raddi (Anacardiaceae). JOURNAL OF APICULTURAL RESEARCH, v. 55, n. 4, p. 315-323, 2016. Web of Science Citations: 0.

CORREA, WALLACE RIBEIRO; LOPEZ, BEGONA GIMENEZ-CASSINA; DO PRADO, SHEILA CRISTINA; DA SILVA CUNHA, ILDENIZE BARBOSA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; SALVADOR, MARCOS JOSE. ESI-MS fingerprinting of residues of green propolis, and evaluation of their antioxidant and antimicrobial activities. JOURNAL OF APICULTURAL RESEARCH, v. 55, n. 1, p. 1-7, 2016. Web of Science Citations: 0.

DOS SANTOS, ADRIANA BROMBINI; BOTTCHER, ALEXANDRA; VICENTINI, RENATO; SAMPAIO MAYER, JULIANA LISCHKA; KIYOTA, EDUARDO; LANDELL, MARCOS A. G.; CRESTE, SILVANA; MAZZAFERA, PAULO. Lignin biosynthesis in sugarcane is affected by low temperature. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 120, p. 31-42, DEC 2015. Web of Science Citations: 8.

MESQUINI, JOSIANE A.; SAWAYA, ALEXANDRA C. H. F.; LOPEZ, BEGONA G. C.; OLIVEIRA, VALERIA M.; MIYASAKA, NATALIA R. S. Detoxification of Atrazine by Endophytic Streptomyces sp Isolated from Sugarcane and Detection of Nontoxic Metabolite. Bulletin of Environmental Contamination and Toxicology, v. 95, n. 6, p. 803-809, DEC 2015. Web of Science Citations: 6.

MORAIS, DAMILA R.; ROTTA, ELIZA M.; SARGI, SHEISA C.; SCHMIDT, EDUARDO M.; BONAFE, ELTON GUNTENDORFER; EBERLIN, MARCOS N.; SAWAYA, ALEXANDRA C. H. F.; VISENTAINER, JESUI V. Antioxidant activity, phenolics and UPLC-ESI(-)-MS of extracts from different tropical fruits parts and processed peels. Food Research International, v. 77, n. 3, p. 392-399, NOV 2015. Web of Science Citations: 27.

MOKOCHINSKI, JOAO BENHUR; BATAGLION, GIOVANA ANCESKI; KIYOTA, EDUARDO; DE SOUZA, LETICIA MARRONE; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 407, n. 23, p. 7221-7227, SEP 2015. Web of Science Citations: 6.

VICENTINI, RENATO; BOTTCHER, ALEXANDRA; BRITO, MICHAEL DOS SANTOS; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; DE ANDRADE LANDELL, MARCOS GUIMARAES; CESARINO, IGOR; MAZZAFERA, PAULO. Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content. PLoS One, v. 10, n. 8 AUG 4 2015. Web of Science Citations: 22.

BRITO, MICHAEL SANTOS; NOBILE, PAULA MACEDO; BOTTCHER, ALEXANDRA; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; ANDRADE DE LANDELL, MARCOS GUIMARES; VINCENTZ, MICHEL; VICENTINI, RENATO; MAZZAFERA, PAULO. Expression Profile of Sugarcane Transcription Factor Genes Involved in Lignin Biosynthesis. TROPICAL PLANT BIOLOGY, v. 8, n. 1-2, p. 19-30, JUN 2015. Web of Science Citations: 8.

DOS SANTOS, ADRIANA BROMBINI; BOTTCHER, ALEXANDRA; KIYOTA, EDUARDO; SAMPAIO MAYER, JULIANA LISCHKA; VICENTINI, RENATO; BRITO, MICHAEL DOS SANTOS; CRESTE, SILVANA; LANDELL, MARCOS G. A.; MAZZAFERA, PAULO. Water Stress Alters Lignin Content and Related Gene Expression in Two Sugarcane Genotypes. Journal of Agricultural and Food Chemistry, v. 63, n. 19, p. 4708-4720, MAY 20 2015. Web of Science Citations: 13.

DE MELO, LUCILIA V.; SAWAYA, ALEXANDRA C. H. F. UHPLC-MS quantification of coumarin and chlorogenic acid in extracts of the medicinal plants known as guaco (Mikania glomerata and Mikania laevigata). REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 25, n. 2, p. 105-110, MAR-APR 2015. Web of Science Citations: 15.

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