Control of lignin biosynthesis in sugar cane: many gaps still to be filled

• Processo: 08/58035-6
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Data de Início da vigência: 01 de setembro de 2009
• Data de Término da vigência: 31 de agosto de 2014
• Área do conhecimento: Ciências Agrárias - Agronomia - Fitotecnia
• Acordo de Cooperação: CNPq - Pronex
• Pesquisador responsável: Paulo Mazzafera
• Beneficiário: Paulo Mazzafera
• Instituição Sede: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brasil
• Auxílio(s) vinculado(s): 13/50315-8 - Identification of target genes regulated by the transcriptions factor shine in monocot plants using chilp-seq, AP.R
• Bolsa(s) vinculada(s): 12/23187-6 - Metabolismo de lignina em quatro genótipos de cana-de-açúcar cultivados em campo, BP.MS; 12/14688-1 - Análise do conteúdo de lignina em duas variedades de cana-de-açúcar expostas a nitrogênio e baixa luminosidade, BP.TT; 12/08471-0 - Análise do perfil de expressão de genes codificadores de enzimas da via da biossíntese da lignina e de proteínas dirigentes em espécies do gênero Saccharum, BP.IC; + mais bolsas vinculadas 11/17081-8 - Biossíntese de lignina em cana-de-açúcar sob estresse por seca e frio, BP.IC; 11/10495-1 - Análise do conteúdo de lignina em duas variedades de cana-de-açúcar expostas a nitrogênio e baixa luminosidade, BP.TT; 11/08207-8 - Anatomia aplicada à expressão dos genes da biossíntese de lignina em cana de açúcar, BP.PD; 11/01656-1 - Expressão de genes da via da biossíntese de lignina em duas variedades de cana-de-açúcar expostas a nitrogênio e baixa luminosidade, BP.TT; 10/11476-8 - Identificação e validação de fatores de transcrição envolvidos com a via de biossíntese de lignina em cana-de-açúcar, BP.PD; 10/08232-0 - Controle da biossíntese de lignina em cana-de-açúcar: família gênica de proteínas dirigente, BP.PD - menos bolsas vinculadas
• Assunto(s): Bioenergia Lignina Cana-de-açúcar Expressão gênica Metabolismo secundário Espectroscopia de massa
• Palavra(s)-Chave do Pesquisador: Cana De Acucar | Espectrometria De Massa | Expressao Genica | Lignina | Metabolismo Secundario
• Publicação FAPESP: https://media.fapesp.br/bv/uploads/publicacoes/pasta_bioen_jun2012_110.pdf

Resumo

Lignin content may vary in response to several biotic and abiotic stresses and understanding how this occurs may help to understand how to control the lignin biosynthesis and consequently its content in plants. Very little is known about lignin metabolism in sugarcane. However, taking in account the information accumulated for other plants and the agronomical practices and problems in sugarcane cultivation, we may have enough hints to plan several studies on how sugarcane modulates lignin composition and content. Therefore, the aim of this project is 1) to cultivate contrasting sugarcane genetic material for lignin content in six locations well characterized for temperature, water availability and irradiance and analyze lignin, sucrose and cellulose, and then, based on these results to study gene expression and perform a more detailed study of lignin composition; 2) to search the SUCEST database for ESTs coding transcription factors known to be involved in lignin metabolism in model plants and use this information in controlled studies (on water supply, nitrogen fertilization, light intensity and low temperatures under field and greenhouse conditions, and growth chamber) to establish correlations between transcription factors regulation and lignin content and composition; 3) search the SUCEST database for ESTs coding ortologs to peroxidases and laccases and use this information in the controlled studies to evaluate the involvement of these enzymes in lignin biosynthesis; 4) to perform a system biology study of regulatory network involved in lignin biosynthesis. To reach these aims we will use a population segregating for lignin content. With this information we may get some valuable knowledge on the lignin biosynthesis in the complex sugarcane genome. Shortly we believe to have at least initial information on how environmental factors affect different sugarcane genotypes. Also it is expected that our results allow us to understand how genes of the lignin biosynthesis pathway and related transcription factors changes lignin content and structure in sugarcane as influenced by some specific environmental factors. In a long-term objective, we may have the possibility to gain knowledge on the agronomical practices may contribute to decrease lignin in sugarcane. This knowledge on the interaction of environmental factors and control of the lignin biosynthesis and related transcription factors may allows us to design a transgenic plants altered in specific genes, aiming not only the decrease of lignin but also its structure. (AU)

Publicações científicas

(52)

(Referências obtidas automaticamente do Web of Science e do SciELO, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores)

ALMEIDA, CLAUDIA DE LAZZARI; XAVIER, RENATA MOURA; BORGHI, ALEXANDRE AUGUSTO; DOS SANTOS, VIVIAN FRANCESCHINI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Effect of seasonality and growth conditions on the content of coumarin, chlorogenic acid and dicaffeoylquinic acids in Mikania laevigata Schultz and Mikania glomerata Sprengel (Asteraceae) by UHPLC-MS/MS. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, n. SI, p. 162-172, JUL 2017. (08/58035-6, 13/15962-2)

CESARINO, IGOR; ARAUJO, PEDRO; DOMINGUES JUNIOR, ADILSON PEREIRA; MAZZAFERA, PAULO. An overview of lignin metabolism and its effect on biomass recalcitrance. BRAZILIAN JOURNAL OF BOTANY, v. 35, n. 4, p. 303-311, OCT-DEC 2012. (08/58035-6)

CHAUHAN, NAGENDRA SINGH; SHARMA, VIKAS; THAKUR, MAYANK; SAWAYA, ALEXANDRA CHRISTINE HELENA FRANKLAND; DIXIT, V. K.. Pueraria tuberosa DC Extract Improves Androgenesis and Sexual Behavior via FSH LH Cascade. SCIENTIFIC WORLD JOURNAL, 2013. (08/58035-6)

RITYELLO S. VOGAS; MARIANA T.M. PEREIRA; LUIZA S. DUARTE; MARA J. CARNEIRO; ANDREW F. FARSURA; JOÃO AUGUSTO M.M. MACHADO; INGRID F. COSTA; MAYARA R.N. TOMÉ; FLORA A. MILTON; FRANCISCO A.R. NEVES; et al. Evaluation of the anti-inflammatory potential of Solidago microglossa (Arnica-brasileira) in vivo and its effects on PPARγ activity. Anais da Academia Brasileira de Ciências, v. 92, n. 2, 2020-08-12. (08/58035-6)

LUCILIA V. DE MELO; ALEXANDRA C.H.F. SAWAYA. UHPLC–MS quantification of coumarin and chlorogenic acid in extracts of the medicinal plants known as guaco (Mikania glomerata and Mikania laevigata). REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 25, n. 2, p. 105-110, 2015-04-00. (08/58035-6)

BOARETTO, LUIS F.; CARVALHO, GISELLE; BORGO, LUCELIA; CRESTE, SILVANA; LANDELL, MARCOS G. A.; MAZZAFERA, PAULO; AZEVEDO, RICARDO A.. Water stress reveals differential antioxidant responses of tolerant and non-tolerant sugarcane genotypes. Plant Physiology and Biochemistry, v. 74, p. 165-175, JAN 2014. (08/58035-6, 09/54676-0)

ARAUJO, PEDRO; FERREIRA, MONICA SIQUEIRA; DE OLIYEIRA, DIOGO NOIN; PEREIRA, LUCIANO; SAWAYA, ALEXANDRA CHRISTINE HELENA FRANK LAND; CATHARINO, RODRIGO RAMOS; MAZZAFERA, PAULO. Mass Spectrometry Imaging: An Expeditious and Powerful Technique for Fast in Situ Lignin Assessment in Eucalyptus. Analytical Chemistry, v. 86, n. 7, p. 3415-3419, APR 1 2014. (08/58035-6)

CORREA, WALLACE RIBEIRO; LOPEZ, BEGONA GIMENEZ-CASSINA; DO PRADO, SHEILA CRISTINA; DA SILVA CUNHA, ILDENIZE BARBOSA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; SALVADOR, MARCOS JOSE. ESI-MS fingerprinting of residues of green propolis, and evaluation of their antioxidant and antimicrobial activities. JOURNAL OF APICULTURAL RESEARCH, v. 55, n. 1, p. 1-7, 2016. (08/58035-6)

MESQUINI, JOSIANE A.; SAWAYA, ALEXANDRA C. H. F.; LOPEZ, BEGONA G. C.; OLIVEIRA, VALERIA M.; MIYASAKA, NATALIA R. S.. Detoxification of Atrazine by Endophytic Streptomyces sp Isolated from Sugarcane and Detection of Nontoxic Metabolite. Bulletin of Environmental Contamination and Toxicology, v. 95, n. 6, p. 803-809, DEC 2015. (08/58035-6, 11/50917-2)

VICENTINI, RENATO; BOTTCHER, ALEXANDRA; BRITO, MICHAEL DOS SANTOS; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; DE ANDRADE LANDELL, MARCOS GUIMARAES; CESARINO, IGOR; MAZZAFERA, PAULO. Large-Scale Transcriptome Analysis of Two Sugarcane Genotypes Contrasting for Lignin Content. PLoS One, v. 10, n. 8, AUG 4 2015. (08/58035-6, 08/58031-0)

BOTTCHER, ALEXANDRA; CESARINO, IGOR; DOS SANTOS, ADRIANA BROMBINI; VICENTINI, RENATO; SAMPAIO MAYER, JULIANA LISCHKA; VANHOLME, RUBEN; MORREEL, KRIS; GOEMINNE, GEERT; MAGALHAES SILVA MOURA, JULLYANA CRISTINA; NOBILE, PAULA MACEDO; et al. Lignification in Sugarcane: Biochemical Characterization, Gene Discovery, and Expression Analysis in Two Genotypes Contrasting for Lignin Content. Plant Physiology, v. 163, n. 4, p. 1539-1557, DEC 2013. (08/58035-6)

COSTA, PATRICIA DE BRITTO; ABRAHAO, ANNA; GORNE VIANI, RICARDO AUGUSTO; SANTIN BRANCALION, PEDRO HENRIQUE; LAMBERS, HANS; HELENA FRANKAND SAWAYA, ALEXANDRA CHRISTINE; OLIVEIRA, RAFAEL S.. Cluster-root formation and carboxylate release in Euplassa cantareirae (Proteaceae) from a neotropical biodiversity hotspot. PLANT AND SOIL, v. 403, n. 1-2, p. 267-275, JUN 2016. (08/58035-6, 10/17204-0, 11/17037-9)

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ALLEVATO, DANIELLA M.; GROPPO, MILTON; KIYOTA, EDUARDO; MAZZAFERA, PAULO; NIXON, KEVIN C.. Evolution of phytochemical diversity in Pilocarpus (Rutaceae). Phytochemistry, v. 163, p. 132-146, JUL 2019. (08/58035-6, 16/06260-2)

SALVATO, FERNANDA; WILSON, RASHAUN; PORTILLA LLERENA, JUAN PABLO; KIYOTA, EDUARDO; REIS, KARINA LIMA; BOARETTO, LUIS FELIPE; BALBUENA, TIAGO S.; AZEVEDO, RICARDO A.; THELEN, JAY J.; MAZZAFERA, PAULO. Luxurious Nitrogen Fertilization of Two Sugar Cane Genotypes Contrasting for Lignin Composition Causes Changes in the Stem Proteome Related to Carbon, Nitrogen, and Oxidant Metabolism but Does Not Alter Lignin Content. JOURNAL OF PROTEOME RESEARCH, v. 16, n. 10, p. 3688-3703, OCT 2017. (16/06236-4, 13/10779-5, 14/25994-1, 08/58035-6)

BRITO, MICHAEL SANTOS; NOBILE, PAULA MACEDO; BOTTCHER, ALEXANDRA; DOS SANTOS, ADRIANA BROMBINI; CRESTE, SILVANA; ANDRADE DE LANDELL, MARCOS GUIMARES; VINCENTZ, MICHEL; VICENTINI, RENATO; MAZZAFERA, PAULO. Expression Profile of Sugarcane Transcription Factor Genes Involved in Lignin Biosynthesis. TROPICAL PLANT BIOLOGY, v. 8, n. 1-2, p. 19-30, JUN 2015. (08/58035-6, 08/58031-0)

MARIANO, BARBARA JARDIM; DE OLIVEIRA, VANESSA SALES; HIDALGO CHAVEZ, DAVY WILLIAM; CASTRO, ROSANE NORA; RIGER, CRISTIANO JORGE; MENDES, JESSICA SODRE; SOUZA, MARCELO DA COSTA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; SAMPAIO, GENI RODRIGUES; FERRAZ DA SILVA TORRES, ELIZABETH APARECIDA; et al. Biquinho pepper (Capsium chinense): Bioactive compounds, in vivo and in vitro antioxidant capacities and anti-cholesterol oxidation kinetics in fish balls during frozen storage. FOOD BIOSCIENCE, v. 47, p. 12-pg., 2022-06-01. (08/58035-6)

ALMEIDA, CLAUDIA DE LAZZARI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; LOPEZ DE ANDRADE, SARA ADRIAN. Mycorrhizal influence on the growth and bioactive compounds composition of two medicinal plants: Mikania glomerata Spreng. and Mikania laevigata Sch Bip. ex Baker (Asteraceae). Rev. bras. Bot., v. 41, n. 1, p. 8-pg., 2018-03-01. (08/58035-6, 13/15962-2)

ALMEIDA, CLAUDIA DE LAZZARI; XAVIER, RENATA MOURA; BORGHI, ALEXANDRE AUGUSTO; DOS SANTOS, VIVIAN FRANCESCHINI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Effect of seasonality and growth conditions on the content of coumarin, chlorogenic acid and dicaffeoylquinic acids in Mikania laevigata Schultz and Mikania glomerata Sprengel (Asteraceae) by UHPLC-MS/MS. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 418, p. 11-pg., 2017-07-01. (08/58035-6, 13/15962-2)

CESARINO, IGOR; ARAUJO, PEDRO; DOMINGUES JUNIOR, ADILSON PEREIRA; MAZZAFERA, PAULO. An overview of lignin metabolism and its effect on biomass recalcitrance. Rev. bras. Bot., v. 35, n. 4, p. 9-pg., 2012-10-01. (08/58035-6)

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DE OLIVEIRA, VANESSA SALES; HIDALGO CHAVES, DAVY WILLIAN; GAMALLO, ORMINDO DOMINGUES; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; SAMPAIO, GENI RODRIGUES; CASTRO, ROSANE NORA; FERRAZ DA SILVA TORRES, ELIZABETH APARECIDA; SALDANHA, TATIANA. Effect of aroeira (Schinus terebinthifolius Raddi) fruit against polyunsaturated fatty acids and cholesterol thermo-oxidation in model systems containing sardine oil (Sardinella brasiliensis). Food Research International, v. 132, JUN 2020. (08/58035-6)

NOBILE, PAULA MACEDO; BOTTCHER, ALEXANDRA; MAYER, JULIANA L. S.; BRITO, MICHAEL S.; DOS ANJOS, IVAN A.; DE ANDRADE LANDELL, MARCOS GUIMARAES; VICENTINI, RENATO; CRESTE, SILVANA; RIANO-PACHON, DIEGO MAURICIO; MAZZAFERA, PAULO. Identification, classification and transcriptional profiles of dirigent domain-containing proteins in sugarcane. Molecular Genetics and Genomics, v. 292, n. 6, p. 1323-1340, DEC 2017. (08/58035-6, 12/08540-1, 10/11476-8, 10/08232-0)

FERREIRA, FERNANDA S.; SAMPAIO, GENI R.; KELLER, LAURA M.; SAWAYA, ALEXANDRA C. H. F.; CHAVEZ, DAVY W. H.; TORRES, ELIZABETH A. F. S.; SALDANHA, TATIANA. Impact of Air Frying on Cholesterol and Fatty Acids Oxidation in Sardines: Protective Effects of Aromatic Herbs. Journal of Food Science, v. 82, n. 12, p. 2823-2831, DEC 2017. (08/58035-6)

DE CARVALHO, ISABELLA OLIVEIRA ALVES MOREIRA; DE OLIVEIRA, VANESSA SALES; CHAVEZ, DAVY WILLIAM HIDALGO; GAMALLO, ORMINDO DOMINGUES; CASTRO, ROSANE NORA; SAWAYA, ALEXANDRA CHRISTINE HELENA FRANKLAND; SAMPAIO, GENI RODRIGUES; TORRES, ELIZABETH APARECIDA FERRAZ DA SILVA; SALDANHA, TATIANA. The use of lemon juice and its role on polyunsaturated fatty acids and cholesterol oxides formation in thermally prepared sardines. Journal of Food Composition and Analysis, v. 104, DEC 2021. (08/58035-6)

DANTAS, NATALIE MARINHO; DE OLIVEIRA, VANESSA SALES; SAMPAIO, GENI RODRIGUES; KOPPE CHRYSOSTOMO, YANE SANE; HIDALGO CHAVEZ, DAVY WILLIAM; GAMALLO, ORMINDO DOMINGUES; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; FERRAZ DA SILVA TORRES, ELIZABETH APARECIDA; SALDANHA, TATIANA. Lipid profile and high contents of cholesterol oxidation products (COPs) in different commercial brands of canned tuna. Food Chemistry, v. 352, AUG 1 2021. (08/58035-6)

MORAIS, DAMILA R.; ROTTA, ELIZA M.; SARGI, SHEISA C.; SCHMIDT, EDUARDO M.; BONAFE, ELTON GUNTENDORFER; EBERLIN, MARCOS N.; SAWAYA, ALEXANDRA C. H. F.; VISENTAINER, JESUI V.. Antioxidant activity, phenolics and UPLC-ESI(-)-MS of extracts from different tropical fruits parts and processed peels. Food Research International, v. 77, n. 3, p. 392-399, NOV 2015. (08/58035-6)

LLERENA, JUAN P. P.; ARAUJO, PEDRO; MAZZAFERA, PAULO. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum. Anais da Academia Brasileira de Ciências, v. 90, n. 1, p. 509-519, MAR 2018. (08/58035-6, 14/25994-1)

ALMEIDA, CLAUDIA DE LAZZARI; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; LOPEZ DE ANDRADE, SARA ADRIAN. Mycorrhizal influence on the growth and bioactive compounds composition of two medicinal plants: Mikania glomerata Spreng. and Mikania laevigata Sch Bip. ex Baker (Asteraceae). BRAZILIAN JOURNAL OF BOTANY, v. 41, n. 1, p. 233-240, MAR 2018. (13/15962-2, 08/58035-6)

BOARETTO, L. F.; MAZZAFERA, P.. The proteomes of feedstocks used for the production of second-generation ethanol: a lacuna in the biofuel era. Annals of Applied Biology, v. 163, n. 1, p. 12-22, 2013. (08/58035-6)

LOPEZ, BEGONA GIMENEZ-CASSINA; SCHMIDT, EDUARDO MORGADO; EBERLIN, MARCOS N.; SAWAYA, ALEXANDRA C. H. F.. Phytochemical markers of different types of red propolis. Food Chemistry, v. 146, p. 174-180, MAR 1 2014. (08/58035-6, 12/03091-4)

VOGAS, RITYELLO S.; PEREIRA, MARIANA T. M.; DUARTE, LUIZA S.; CARNEIRO, MARA J.; FARSURA, ANDREW F.; MACHADO, JOAO AUGUSTO M. M.; COSTA, INGRID F.; TOME, MAYARA R. N.; MILTON, FLORA A.; NEVES, FRANCISCO A. R.; et al. Evaluation of the anti-inflammatory potential of Solidago microglossa (Arnica-brasileira) in vivo and its effects on PPAR gamma activity. Anais da Academia Brasileira de Ciências, v. 92, n. 2, 2020. (08/58035-6)

PORTILLA LLERENA, JUAN PABLO; FIGUEIREDO, RAQUEL; BRITON, MICHAEL DOS SANTOS; KIYOTA, EDUARDO; SAMPAIO MAYER, JULIANA LISCHKA; ARAUJO, PEDRO; SCHIMPL, FLAVIA CAMILA; DAMA, MURALI; PAULY, MARKUS; MAZZAFERA, PAULO. Deposition of lignin in four species of Saccharum. SCIENTIFIC REPORTS, v. 9, APR 10 2019. (14/25994-1, 08/58035-6)

SALVATO, FERNANDO; LOZIUK, PHILIP; KIYOTA, EDUARDO; DANELUZZI, GABRIEL SILVA; ARAUJO, PEDRO; MUDDIMAN, DAVID C.; MAZZAFERA, PAULO. Label-Free Quantitative Proteomics of Enriched Nuclei from Sugarcane (Saccharum ssp) Stems in Response to Drought Stress. PROTEOMICS, v. 19, n. 14, JUL 2019. (16/06236-4, 13/10779-5, 14/25994-1, 08/58035-6)

MOKOCHINSKI, JOAO BENHUR; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; MUMM, ROLAND; HENDRICUS DE VOS, RIC CORNELIS; HALL, ROBERT DAVID. Metabolic responses of Eucalyptus species to different temperature regimes. JOURNAL OF INTEGRATIVE PLANT BIOLOGY, v. 60, n. 5, p. 397-411, MAY 2018. (08/58035-6, 11/51949-5, 13/21306-0, 15/06987-7)

DE OLIVEIRA COSTA, VANESSA CRUZ; BORGHI, ALEXANDRE AUGUSTO; SAMPAIO MAYER, JULIANA LISCHKA; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. Comparison of the Morphology, Anatomy, and Chemical Profile of Mikania glomerata and Mikania laevigata. Planta Medica, v. 84, n. 3, p. 191-200, FEB 2018. (08/58035-6)

CARNEIRO, M. J.; LOPEZ, B. G-C; LANCELLOTTI, M.; FRANCHI, G. C.; NOWILL, ALEXANDRE E.; SAWAYA, A. C. H. F.. Evaluation of the chemical composition and biological activity of extracts of Tetragonisca angustula propolis and Schinus terebinthifolius Raddi (Anacardiaceae). JOURNAL OF APICULTURAL RESEARCH, v. 55, n. 4, p. 315-323, 2016. (15/06215-4, 13/20313-3, 08/58035-6)

DOS SANTOS, ADRIANA BROMBINI; BOTTCHER, ALEXANDRA; VICENTINI, RENATO; SAMPAIO MAYER, JULIANA LISCHKA; KIYOTA, EDUARDO; LANDELL, MARCOS A. G.; CRESTE, SILVANA; MAZZAFERA, PAULO. Lignin biosynthesis in sugarcane is affected by low temperature. ENVIRONMENTAL AND EXPERIMENTAL BOTANY, v. 120, p. 31-42, DEC 2015. (08/58035-6)

MOKOCHINSKI, JOAO BENHUR; BATAGLION, GIOVANA ANCESKI; KIYOTA, EDUARDO; DE SOUZA, LETICIA MARRONE; MAZZAFERA, PAULO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE. A simple protocol to determine lignin S/G ratio in plants by UHPLC-MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 407, n. 23, p. 7221-7227, SEP 2015. (08/58035-6, 11/51949-5, 13/21306-0, 12/21395-0)

DE MELO, LUCILIA V.; SAWAYA, ALEXANDRA C. H. F.. UHPLC-MS quantification of coumarin and chlorogenic acid in extracts of the medicinal plants known as guaco (Mikania glomerata and Mikania laevigata). REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, v. 25, n. 2, p. 105-110, MAR-APR 2015. (08/58035-6)

ARAUJO, PEDRO; CESARINO, IGOR; SAMPAIO MAYER, JULIANA LISCHKA; FERRARI, ILSE FERNANDA; KIYOTA, EDUARDO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; PAES LEME, ADRIANA FRANCO; MAZZAFERA, PAULO. A model system to study the lignification process in Eucalyptus globulus. PHYSIOLOGIA PLANTARUM, v. 152, n. 1, p. 17-31, SEP 2014. (08/58035-6)

CESARINO, IGOR; ARAUJO, PEDRO; PAES LEME, ADRIANA FRANCO; CRESTE, SILVANA; MAZZAFERA, PAULO. Suspension cell culture as a tool for the characterization of class III peroxidases in sugarcane. Plant Physiology and Biochemistry, v. 62, p. 1-10, JAN 2013. (08/58035-6)

CESARINO, IGOR; ARAUJO, PEDRO; SAMPAIO MAYER, JULIANA LISCHKA; VICENTINI, RENATO; BERTHET, SERGE; DEMEDTS, BRECHT; VANHOLME, BARTEL; BOERJAN, WOUT; MAZZAFERA, PAULO. Expression of SofLAC, a new laccase in sugarcane, restores lignin content but not S:G ratio of Arabidopsis lac17 mutant. Journal of Experimental Botany, v. 64, n. 6, p. 1769-1781, APR 2013. (08/58035-6)

DOS SANTOS, ADRIANA BROMBINI; BOTTCHER, ALEXANDRA; KIYOTA, EDUARDO; SAMPAIO MAYER, JULIANA LISCHKA; VICENTINI, RENATO; BRITO, MICHAEL DOS SANTOS; CRESTE, SILVANA; LANDELL, MARCOS G. A.; MAZZAFERA, PAULO. Water Stress Alters Lignin Content and Related Gene Expression in Two Sugarcane Genotypes. Journal of Agricultural and Food Chemistry, v. 63, n. 19, p. 4708-4720, MAY 20 2015. (08/58035-6)

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FERREIRA, FERNANDA SILVA; DE OLIVEIRA, VANESSA SALES; HIDALGO CHAVEZ, DAVY WILLIAM; CHAVES, DOUGLAS SIQUEIRA; RIGER, CRISTIANO JORGE; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; GUIZELLINI, GLORIA MARIA; SAMPAIO, GENI RODRIGUES; FERRAZ DA SILVA TORRES, ELIZABETH APARECIDA; SALDANHA, TATIANA. ioactive compounds of parsley (Petroselinum crispum), chives (Allium schoenoprasum L) and their mixture (Brazilian cheiro-verde) as promising antioxidant and anti-cholesterol oxidation agents in a food syste. Food Research International, v. 151, JAN 2022. (08/58035-6)

DE OLIVEIRA, VANESSA SALES; AUGUSTA, IVANILDA MARIA; DA CONCEICAO BRAZ, MARCOS VINICIUS; RIGER, CRISTIANO JORGE; PRUDENCIO, EDLENE RIBEIRO; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; SAMPAIO, GENI RODRIGUES; FERRAZ DA SILVA TORRES, ELIZABETH APARECIDA; SALDANHA, TATIANA. Aroeira fruit (Schinus terebinthifolius Raddi) as a natural antioxidant: Chemical constituents, bioactive compounds and in vitro and in vivo antioxidant capacity. Food Chemistry, v. 315, JUN 15 2020. (08/58035-6)

RIBEIRO DE SOUZA, SARAH CAROLINE; SODEK, LADASLAV; POLACCO, JOE CARMINE; MAZZAFERA, PAULO. Urease deficiency alters nitrogen metabolism and gene expression in urease-null soybean without affecting growth or productivity under nitrate supply. ACTA PHYSIOLOGIAE PLANTARUM, v. 42, n. 3, FEB 13 2020. (08/58035-6, 13/25094-8, 14/09730-4)

LLERENA, JUAN P. P.; ARAUJO, PEDRO; MAZZAFERA, PAULO. Optimization of RT-PCR reactions in studies with genes of lignin biosynthetic route in Saccharum spontaneum. Anais da Academia Brasileira de Ciências, v. 90, n. 1, p. 11-pg., 2018-03-01. (08/58035-6, 14/25994-1)

CUNHA, CLAUDIO A.; LIRA, FABIO S.; ROSA NETO, JOSE C.; PIMENTEL, GUSTAVO D.; SOUZA, GABRIEL I. H.; GONCALVES DA SILVA, CAMILA MORAIS; DE SOUZA, CLAUDIO T.; RIBEIRO, ELIANE B.; HELENA FRANKLAND SAWAYA, ALEXANDRA CHRISTINE; OLLER DO NASCIMENTO, CLAUDIA M.; et al. Green Tea Extract Supplementation Induces the Lipolytic Pathway, Attenuates Obesity, and Reduces Low-Grade Inflammation in Mice Fed a High-Fat Diet. Mediators of Inflammation, v. 2013, p. 8-pg., 2013-01-01. (08/58035-6)

CHAUHAN, NAGENDRA SINGH; SHARMA, VIKAS; THAKUR, MAYANK; SAWAYA, ALEXANDRA CHRISTINE HELENA FRANKLAND; DIXIT, V. K.. Pueraria tuberosa DC Extract Improves Androgenesis and Sexual Behavior via FSH LH Cascade. SCIENTIFIC WORLD JOURNAL, v. N/A, p. 8-pg., 2013-01-01. (08/58035-6)