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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

A model system to study the lignification process in Eucalyptus globulus

Texto completo
Araujo, Pedro [1] ; Cesarino, Igor [2, 3, 1] ; Sampaio Mayer, Juliana Lischka [1] ; Ferrari, Ilse Fernanda [1] ; Kiyota, Eduardo [1] ; Helena Frankland Sawaya, Alexandra Christine [1] ; Paes Leme, Adriana Franco [4] ; Mazzafera, Paulo [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil
[2] VIB, Dept Plant Syst Biol, B-9052 Ghent - Belgium
[3] Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent - Belgium
[4] CNPEM ABTLuS, Lab Nacl Biociencias, Campinas, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: PHYSIOLOGIA PLANTARUM; v. 152, n. 1, p. 17-31, SEP 2014.
Citações Web of Science: 4

Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus. (AU)

Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
Beneficiário:Paulo Mazzafera
Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático