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Development of model systems in sorghum and a systems biology approach to unravel the molecular mechanisms controlling lignin metabolism in grasses

Abstract

Recently, interest on lignin research has increased significantly since several studies have identified this biopolymer as the main factor responsible for the recalcitrance of cell walls to cellulose-to-glucose conversion for downstream applications, such as the production of biofuels and bio-based materials. Despite recent advances, most of our current knowledge of cell wall formation and lignin metabolism is primarily derived from dicotyledonous species. However, the molecular mechanisms controlling different aspects of lignin metabolism in grasses, such as transcriptional regulation, biosynthesis and polymerization, remains largely unknown, despite of the economic importance of this group of plants. Therefore, comprehensive and systematic studies are needed to unravel specific characteristics of secondary cell wall formation and lignin metabolism in grasses. The present project aims to develop model systems of sorghum and use them in a systems biology approach based on transcriptomics and metabolomics to correlate changes in cell wall composition with changes in gene expression, with a major focus on lignin and phenolic metabolism. The proposed model systems are: i) the elongating internodes system, and ii) inducible cell suspension culture for tracheary element differentiation. Both systems will be characterized for lignin deposition and phenolic profiling and subsequently used in high-throughput transcriptomic analysis using RNAseq. Finally, the transcriptomic data will be used to generate a co-expression network and a database of co-expressed genes, in order to select candidate genes that potentially play a role in secondary cell wall formation and lignin biosynthesis in grasses. The obtained knowledge might be very helpful for future biotechnological strategies to reduce biomass recalcitrance in bioenergy crops, such as sugarcane. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:

Scientific publications (17)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LI, ZHENG; ZHANG, CHUNTANG; ZHANG, YUAN; ZENG, WEI; CESARINO, IGOR. Coffee cell walls-composition, influence on cup quality and opportunities for coffee improvements. FOOD QUALITY AND SAFETY, v. 5, JUN 5 2021. Web of Science Citations: 0.
SUNG, BIANCA MEEIHUA; CARVALHO, GABRIEL GARON; WAIRICH, ANDRIELE; CESARINO, IGOR. Searching for Novel Transcriptional Regulators of Lignin Deposition Within the PIRIN Family in the Model C4 Grass Setaria Viridis. TROPICAL PLANT BIOLOGY, v. 14, n. 2 MAR 2021. Web of Science Citations: 0.
SIMOES, MARCELLA SIQUEIRA; FERREIRA, SAVIO SIQUEIRA; GRANDIS, ADRIANA; RENCORET, JORGE; PERSSON, STAFFAN; FLOH, ENY IOCHEVET SEGAL; FERRAZ, ANDRE; DEL RIO, JOSE C.; BUCKERIDGE, MARCOS SILVEIRA; CESARINO, IGOR. Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming. FRONTIERS IN PLANT SCIENCE, v. 11, DEC 18 2020. Web of Science Citations: 1.
FIGUEIREDO, RAQUEL; PORTILLA LLERENA, JUAN PABLO; KIYOTA, EDUARDO; FERREIRA, SAVIO SIQUEIRA; CARDELI, BARBARA ROCHA; RIBEIRO DE SOUZA, SARAH CAROLINE; BRITO, MICHAEL DOS SANTOS; SODEK, LADASLAV; CESARINO, IGOR; MAZZAFERA, PAULO. The sugarcane ShMYB78 transcription factor activates suberin biosynthesis inNicotiana benthamiana. Plant Molecular Biology, v. 104, n. 4-5 AUG 2020. Web of Science Citations: 0.
CESARINO, IGOR; DELLO IOIO, RAFFAELE; KIRSCHNER, GWENDOLYN K.; OGDEN, MICHAEL S.; PICARD, KELSEY L.; RAST-SOMSSICH, I, MADLEN; SOMSSICH, MARC. Plant science's next top models. ANNALS OF BOTANY, v. 126, n. 1, p. 1-23, JUN 29 2020. Web of Science Citations: 4.
SIMOES, MARCELLA SIQUEIRA; CARVALHO, GABRIEL GARON; FERREIRA, SAVIO SIQUEIRA; HERNANDES-LOPES, JOSE; DE SETTA, NATHALIA; CESARINO, IGOR. Genome-wide characterization of the laccase gene family in Setaria viridis reveals members potentially involved in lignification. PLANTA, v. 251, n. 2 JAN 9 2020. Web of Science Citations: 0.
ARAUJO, PEDRO; TOLENTINO, FELIPE THADEU; CESARINO, IGOR; GALLINARI, RAFAEL HENRIQUE; STEENACKERS, WARD; SAMPAIO MAYER, JULIANA LISCHKA; MAZZAFERA, PAULO. Expression of Eucalyptus globulus LACCASE48 Restores Lignin Content of Arabidopsis thaliana lac17 Mutant. PLANT MOLECULAR BIOLOGY REPORTER, v. 37, n. 5-6, p. 488-498, DEC 2019. Web of Science Citations: 0.
FERREIRA, SAVIO SIQUEIRA; SIMOES, MARCELLA SIQUEIRA; CARVALHO, GABRIEL GARON; ALVES DE LIMA, LEYDSON GABRIEL; DE ALMEIDA SVARTMAN, RAPHAEL MENDES; CESARINO, IGOR. The lignin toolbox of the model grass Setaria viridis. Plant Molecular Biology, v. 101, n. 3, p. 235-255, OCT 2019. Web of Science Citations: 1.
OZPARPUCU, MERVE; GIERLINGER, NOTBURGA; CESARINO, IGOR; BURGERT, INGO; BOERJAN, WOUT; RUGGEBERG, MARKUS. Significant influence of lignin on axial elastic modulus of poplar wood at low microfibril angles under wet conditions. Journal of Experimental Botany, v. 70, n. 15, p. 4039-4047, AUG 1 2019. Web of Science Citations: 1.
CESARINO, IGOR. Structural features and regulation of lignin deposited upon biotic and abiotic stresses. CURRENT OPINION IN BIOTECHNOLOGY, v. 56, p. 209-214, APR 2019. Web of Science Citations: 4.
SORIANO SALEME, MARINA DE LYRA; CESARINO, IGOR; VARGAS, LIVIA; KIM, HOON; VANHOLME, RUBEN; GOEMINNE, GEERT; VAN ACKER, REBECCA; DE ASSIS FONSECA, FERNANDO CAMPOS; PALLIDIS, ANDREAS; VOOREND, WANNES; NICOMEDES JUNIOR, JOSE; PADMAKSHAN, DHARSHANA; VAN DOORSSELAERE, JAN; RALPH, JOHN; BOERJAN, WOUT. Silencing CAFFEOYL SHIKIMATE ESTERASE Affects Lignification and Improves Saccharification in Poplar. Plant Physiology, v. 175, n. 3, p. 1040-1057, NOV 2017. Web of Science Citations: 22.
OZPARPUCU, MERVE; RUGGEBERG, MARKUS; GIERLINGER, NOTBURGA; CESARINO, IGOR; VANHOLME, RUBEN; BOERJAN, WOUT; BURGERT, INGO. Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD). Plant Journal, v. 91, n. 3, p. 480-490, AUG 2017. Web of Science Citations: 15.
ELOY, NUBIA B.; VOOREND, WANNES; LAN, WU; SALEME, MARINA DE LYRA SORIANO; CESARINO, IGOR; VANHOLME, RUBEN; SMITH, REBECCA A.; GOEMINNE, GEERT; PALLIDIS, ANDREAS; MORREEL, KRIS; NICOMEDES, JR., JOSE; RALPH, JOHN; BOERJAN, WOUT. Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content. Plant Physiology, v. 173, n. 2, p. 998-1016, FEB 2017. Web of Science Citations: 23.
STEENACKERS, WARD; KLIMA, PETR; QUARESHY, MUSSA; CESARINO, IGOR; KUMPF, ROBERT P.; CORNEILLIE, SANDER; ARAUJO, PEDRO; VIAENE, TOM; GOEMINNE, GEERT; NOWACK, MORITZ K.; LJUNG, KARIN; FRIML, JIRI; BLAKESLEE, JOSHUA J.; NOVAK, ONDREJ; ZAZIMALOVA, EVA; NAPIER, RICHARD; BOERJAN, WOUT; VANHOLME, BARTEL. cis-Cinnamic Acid Is a Novel, Natural Auxin Efflux Inhibitor That Promotes Lateral Root Formation. Plant Physiology, v. 173, n. 1, p. 552-565, JAN 2017. Web of Science Citations: 15.
CESARINO, IGOR; SIMOES, MARCELLA SIQUEIRA; BRITO, MICHAEL DOS SANTOS; FANELLI, AMANDA; SILVA, TATIANE DA FRANCA; ROMANEL, ELISSON. Building the wall: recent advances in understanding lignin metabolism in grasses. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 11 NOV 2016. Web of Science Citations: 9.
STEENACKERS, WARD; CESARINO, IGOR; KLIMA, PETR; QUARESHY, MUSSA; VANHOLME, RUBEN; CORNEILLIE, SANDER; KUMPF, ROBERT PETER; VAN DE WOUWER, DORIEN; LJUNG, KARIN; GOEMINNE, GEERT; NOVAK, ONDREJ; ZAZIMALOVA, EVA; NAPIER, RICHARD; BOERJAN, WOUT; VANHOLME, BARTEL. The Allelochemical MDCA Inhibits Lignification and Affects Auxin Homeostasis. Plant Physiology, v. 172, n. 2, p. 874-888, OCT 2016. Web of Science Citations: 8.
FIGUEIREDO, RAQUEL; CESARINO, IGOR; MAZZAFERA, PAULO. Suberin as an Extra Barrier to Grass Digestibility: a Closer Look to Sugarcane Forage. TROPICAL PLANT BIOLOGY, v. 9, n. 2, p. 96-108, JUN 2016. Web of Science Citations: 1.

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