Fluorescent antibody test, quantitative polymerase chain reaction pattern and clinical aspects of rabies virus strains isolated from main reservoirs in Brazil

Autor(es)

Appolinario, Camila ^[1] ; Allendorf, Susan Dora ^[1] ; Vicente, Acacia Ferreira ^[1] ; Ribeiro, Bruna Devide ^[1] ; da Fonseca, Clovis Reinaldo ^[1] ; Antunes, Joao Marcelo ^[1] ; Peres, Marina Gea ^[1] ; Kotait, Ivanete ^[2] ; Carrieri, Maria Luiza ^[3] ; Megid, Jane ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP, Fac Med Vet & Zootecnia, Dept Higiene Vet & Saude Publ, Botucatu, SP - Brazil

^[2] Inst Biol Sao Paulo, Sao Paulo, SP - Brazil

^[3] Secretaria Saude, Inst Butantan, Seccao Raiva, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Infectious Diseases; v. 19, n. 5, p. 479-485, SEP-OCT 2015.
• Citações Web of Science: 6

Resumo

ABSTRACTRabies virus (RABV) isolated from different mammals seems to have unique characteristics that influence the outcome of infection. RABV circulates in nature and is maintained by reservoirs that are responsible for the persistence of the disease for almost 4000 years. Considering the different pattern of pathogenicity of RABV strains in naturally and experimentally infected animals, the aim of this study was to analyze the characteristics of RABV variants isolated from the main Brazilian reservoirs, being related to a dog (variant 2),Desmodus rotundus (variant 3), crab eating fox, marmoset, and Myotis spp. Viral replication in brain tissue of experimentally infected mouse was evaluated by two laboratory techniques and the results were compared to clinical evolution from five RABV variants. The presence of the RABV was investigated in brain samples by fluorescent antibody test (FAT) and real time polymerase chain reaction (qRT-PCR) for quantification of rabies virus nucleoprotein gene (N gene). Virus replication is not correlated with clinical signs and evolution. The pattern of FAT is associated with RABV replication levels. Virus isolates from crab eating fox and marmoset had a longer evolution period and higher survival rate suggesting that the evolution period may contribute to the outcome. RABV virus variants had independent characteristics that determine the clinical evolution and survival of the infected mice. (AU)

• Processo FAPESP: 12/00896-1 - Resposta imune inata e antiviral em sistema nervoso central de camundongos experimentalmente infectados com amostras de vírus rábico isoladas de diferentes espécies
• Beneficiário: Susan Dora Allendorf
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/00895-5 - Resposta imune inata e antiviral em sistema nervoso central de camundongos experimentalmente infectados com amostras de vírus rábico isoladas de diferentes espécies
• Beneficiário: Jane Megid
• Modalidade de apoio: Auxílio à Pesquisa - Regular