Gonadotropins and Growth Hormone Family Characterization in an Endangered Siluriform Species, Steindachneridion parahybae (Pimelodidae): Relationship With Annual Reproductive Cycle and Induced Spawning in Captivity

Autor(es)

Honji, Renato Massaaki ^{[1, 2, 3]} ; Caneppele, Danilo ^[4] ; Pandolfi, Matias ^{[1, 3]} ; lo Nostro, Fabiana Laura ^{[1, 3]} ; Moreira, Renata Guimaraes ^[2]

Afiliação do(s) autor(es)

^[1] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Biodiversidad & Biol Expt, Buenos Aires, DF - Argentina

^[2] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, BR-05508090 Sao Paulo, SP - Brazil

^[3] CONICET UBA, IBBEA, Buenos Aires, DF - Argentina

^[4] CESP, Unidade Hidrobiol & Aquicultura, BR-12260000 Paraibuna - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology; v. 298, n. 9, p. 1644-1658, SEP 2015.
• Citações Web of Science: 4

Resumo

The aim of this study was to identify and characterize pituitary cells of Steindachneridion parahybae females in captivity, highlighting the possible relationship with reproductive disorders at this level, since this species shows oocyte final maturation, ovulation and spawning dysfunction in captivity. The localization and distribution of growth hormone (GH), prolactin (PRL), somatolactin (SL), beta-luteinizing hormone (beta-LH), and beta-follicle stimulating hormone (beta-FSH) immunoreactive (-ir) cells in the adenohypophysis was studied by immunohistochemical and Western blot methods. In addition, cellular morphometric analyses and semi-quantification of ir-cells optical density (OD) during the annual reproductive cycle and after artificial induced spawning (AIS) were performed. Results showed that the distribution and general localization of pituitary cell types were similar to that of other teleost species. However, the morphometrical study of adenohypophysial cells showed differences along the reproductive cycle and following AIS. In general, females at the vitellogenic stage presented greater OD values for GH, PRL and SL than at other maturation stages (previtellogenic and regression stages), probably indicating an increased cellular activity during this stage. Conversely, beta-LH OD did not vary during the annual reproductive cycle. After AIS, beta-LH, SL and GH ir-cells showed an increase in OD values suggesting a possible involvement on oocyte final maturation, ovulation and spawning or a feedback control on the brain-pituitary-gonads axis. Reproductive dysfunction in S. parahybae females in captivity may be due to alteration of the synthesis pathways of beta-LH. In addition, GH family of hormones could modulate associated mechanisms that influence the reproductive status in this species. (C) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 08/57687-0 - Effects of global climate change of the Brazilian fauna: a conservation physiology approach
• Beneficiário: Carlos Arturo Navas Iannini
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 07/55494-7 - Controle do eixo hipotálamo-hipófise-gônadas do surubim do Paraíba Steindachneridion Parahybae (Siluriformes: Pimelodidae) em relação ao ciclo reprodutivo e à reprodução induzida em cativeiro
• Beneficiário: Renato Massaaki Honji
• Modalidade de apoio: Bolsas no Brasil - Doutorado