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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Three-dimensional non-destructive soft-tissue visualization with X-ray staining micro-tomography

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Autor(es):
Silva, Juliana Martins de S. e [1, 2] ; Zanette, Irene [2, 3] ; Noel, Peter B. [2, 4] ; Cardoso, Mateus B. [1] ; Kimm, Melanie A. [4] ; Pfeiffer, Franz [2, 4]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Brazilian Synchrotron Light Lab, BR-13083970 Campinas, SP - Brazil
[2] Tech Univ Munich, Dept Phys, Lehrstuhl Biomed Phys, D-85748 Garching - Germany
[3] Diamond Light Source, Didcot OX11 0DE, Oxon - England
[4] Tech Univ Munich, Dept Radiol, D-81675 Munich - Germany
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 5, SEP 25 2015.
Citações Web of Science: 25
Resumo

Low inherent contrast in soft tissues has been limiting the use of X-ray absorption micro-computed tomography (micro-CT) to access high-resolution structural information of animal organs. The staining agents used in micro-CT to improve the contrast fail in providing high-quality images of whole organs of animals due to diffusion problems of the staining agent into the sample. We demonstrate a staining protocol that incorporates a biochemical conditioning step prior to exposure to the staining agent that succeeds in overcoming the diffusion problems, thus quickly providing high-quality micro-CT images of whole organs of mammals. Besides of yielding non-distorted three-dimensional information at the same spatial resolution accessible in histological sections, micro-CT images of whole organs stained by our method enable easy screening of slices along any direction of the volume thus demonstrating new possibilities of structural analysis in biomedical science. (AU)

Processo FAPESP: 13/12190-9 - Entendendo a atividade de nanopartículas funcionalizadas contra vírus através de imageamento por difração coerente
Beneficiário:Juliana Martins de Souza e Silva
Linha de fomento: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado