Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

Autor(es)

Casarin Penha Filho, Rafael Antonio ^{[1, 2]} ; Acelas Diaz, Silvia Juliana ^[1] ; Fernando, Filipe Santos ^[1] ; Chang, Yung-Fu ^[2] ; Andreatti Filho, Raphael Lucio ^[3] ; Berchieri Junior, Angelo ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, BR-14884900 Sao Paulo, SP - Brazil

^[2] Cornell Univ, Coll Vet Med, Ithaca, NY 14850 - USA

^[3] Sao Paulo State Univ UNESP, Sch Vet Med & Anim, BR-18618970 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 167, n. 1-2, p. 64-69, SEP 15 2015.
• Citações Web of Science: 15

Resumo

Lactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L acidophilus, L. fermentum, L. reuteri, L. salivarius). The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, gamma delta, CD4(+) and CD8(+) T cells in the gut. The intestinal colonization by SE was reduced by 1.8 CFU/g (log(10)) in chicks treated with LBP (p < 0.05). The levels of pro-inflammatory cytokines (IL-1 beta, LITAF) were significantly reduced in treated chicks (p < 0.05), whilst untreated chicks showed elevated inflammatory stimulus and an increased population of CD8(+) T cells in the intestinal mucosa after challenge (p < 0.05). Additionally, the LBP stimulated TLR2 expression in caecal tonsils. The adjuvant property of the Lactobacillus cell wall (LCW) was evaluated, demonstrating good capability to stimulate T helper 2 (Th2) cell proliferation. Pretreatment of chicks with LBP decreased the intestinal colonization by SE, minimizing the tissue lesions and inflammation after challenge and showed a potential use as adjuvant with injectable killed vaccines. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 13/26127-7 - Fatores envolvidos na patogenia do tifo aviário
• Beneficiário: Angelo Berchieri Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/24017-7 - Avaliação da infecção e imunidade de linhagens de aves (Gallus gallus) suscetíveis e resistentes ao desafio por Salmonella enterica subesp. enterica sorovar Gallinarum
• Beneficiário: Rafael Antonio Casarin Penha Filho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado