Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

Author(s)

Casarin Penha Filho, Rafael Antonio ^{[1, 2]} ; Acelas Diaz, Silvia Juliana ^[1] ; Fernando, Filipe Santos ^[1] ; Chang, Yung-Fu ^[2] ; Andreatti Filho, Raphael Lucio ^[3] ; Berchieri Junior, Angelo ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, BR-14884900 Sao Paulo, SP - Brazil

^[2] Cornell Univ, Coll Vet Med, Ithaca, NY 14850 - USA

^[3] Sao Paulo State Univ UNESP, Sch Vet Med & Anim, BR-18618970 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY; v. 167, n. 1-2, p. 64-69, SEP 15 2015.
• Web of Science Citations: 15

Abstract

Lactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L acidophilus, L. fermentum, L. reuteri, L. salivarius). The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, gamma delta, CD4(+) and CD8(+) T cells in the gut. The intestinal colonization by SE was reduced by 1.8 CFU/g (log(10)) in chicks treated with LBP (p < 0.05). The levels of pro-inflammatory cytokines (IL-1 beta, LITAF) were significantly reduced in treated chicks (p < 0.05), whilst untreated chicks showed elevated inflammatory stimulus and an increased population of CD8(+) T cells in the intestinal mucosa after challenge (p < 0.05). Additionally, the LBP stimulated TLR2 expression in caecal tonsils. The adjuvant property of the Lactobacillus cell wall (LCW) was evaluated, demonstrating good capability to stimulate T helper 2 (Th2) cell proliferation. Pretreatment of chicks with LBP decreased the intestinal colonization by SE, minimizing the tissue lesions and inflammation after challenge and showed a potential use as adjuvant with injectable killed vaccines. (C) 2015 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 13/26127-7 - Factors involved in the pathogenesis of fowl typhoid
• Grantee: Angelo Berchieri Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/24017-7 - Evaluation of the infection and immunity in susceptible and resistant lines of chicken (Gallus gallus) challenged with Salmonella enterica subsp. enterica sorovar Gallinarum
• Grantee: Rafael Antonio Casarin Penha Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral