Effects of Lactobacillus spp. on cytokine, avian defensins e toll-like receptors gene expression of heat-stressed broiler chickens infected with Salmonella Typhimurium

• Grant number: 15/00987-5
• Support type: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): April 30, 2015
• Effective date (End): April 29, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal researcher: João Palermo Neto
• Grantee: Wanderley Moreno Quinteiro Filho
• Supervisor abroad: Shayan Sharif
• Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Research place: University of Guelph, Canada
• Associated to the scholarship: 13/13736-5 - Effects of Lactobacillus salivarius on immunity and intestinal commensal microbiota of heat-stressed broiler chickens infected with Salmonella Enteritidis, BP.PD

Abstract

Environmental stressors, i.e heat stress, impair welfare, performance parameters and immunity in broiler chickens. The neuroimmunomodulation is defined as the interrelationships between the Central Nervous System and the Immune System, acting in the HPA axis releasing corticosterone and in Sympathetic nervous system releasing noradrenaline. Moreover, stressful events have been associated with disruption of intestinal commensal microbiota. We observed that the heat stress decreased performance parameters, relative weight of lymphoid organs, the macrophage activity and increased corticosterone serum level and intestinal inflammation. When chickens were experimentally infected with Salmonella Enteritidis or Samonella Typhimurium, we observed an increase of Salmonella counts in the spleens, liver and caecum of the heat-stressed chickens. The probiotics have been used as an alternative to antimicrobial growth promoters for control food-borne pathogens (ie Salmonella). The Lactobacillus is one of the most important representatives of probiotics and it is capable of modulating immune responses in humans and birds. In this way, the objective of the current study is to examine the effects of Lactobacillus salivarius, Lactobacillus acidophilus and Lactobacillus reuteri on cytokine, AVBD and TLR expression in heat stressed broiler chickens experimentally infected with Salmonella Typhimurium. We also propose analyze the role of in vitro noradrenaline on chicken macrophage activity treated with Lactobacillus. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUINTEIRO-FILHO, W. M.; BRISBIN, J. T.; HODGINS, D. C.; SHARIF, S. Lactobacillus and Lactobacillus cell-free culture supernatants modulate chicken macrophage activities. Research in Veterinary Science, v. 103, p. 170-175, DEC 2015. Web of Science Citations: 3.