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Evaluation of the vaccine potential of recombinant proteins for protection against Salmonella enterica and the use of avian (Gallus gallus) cytokines as adjuvants and imunotherapics

Grant number: 16/12293-0
Support type:Research Grants - Young Investigators Grants
Duration: March 01, 2017 - February 28, 2021
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Rafael Antonio Casarin Penha Filho
Grantee:Rafael Antonio Casarin Penha Filho
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):19/04284-0 - Evaluation of the use of avian (Gallus Gallus) cytokine CCl4 as imunotherapic in the control of fowl typhoid (Salmonella Gallinarum), BP.IC
17/00620-0 - Evaluation of the vaccine potential of recombinant proteins for protection against Salmonella enterica and the use of avian (Gallus Gallus) cytokines as adjuvants and imunotherapics, BP.JP


Currently, Salmonella enterica is one of the most important pathogens for the poultry industry due to growing concerns about food safety and public health. Brazil is the largest exporter in the world of chicken meat and the 2nd largest producer of food of poultry origin. Therefore, this activity has to meet many requirements involving the control of this pathogen. The control of Salmonella enterica infections in poultry, is done by biosecurity measures, antimicrobials and vaccines. However, a large number of antibiotic multidrug-resistant isolates has become disseminated in animal production environment and the antimicrobials used in food-producing animals have reduced or no action on these isolates. There are over 2,600 serotypes of Salmonella described, but live and inactivated vaccines available in Brazil are licensed to control only a few serovars (Salmonella gallinarum, Salmonella enteritidis and Salmonella typhimurium). Thus, this project proposes the evaluation of a "pool" of immunogenic proteins with high homology between serovars of the three major serogroups of Salmonella enterica (B, C and D), as a vaccine candidate for cross-protection against different serovars, relevant for poultry and public health. In addition, interleukin-12 (IL-12), Flagellin (FliC) and nanoparticles will be evaluated as adjuvant molecules of the inactivated subunit vaccine, to stimulate the cellular immune response. Considering the emergence of Salmonella enterica isolates multiresistant to antibiotics, this project also proposes the evaluation of CCL4 chemokine cloned from chickens, as an immunotherapic for the attraction of leukocytes to the intestinal mucosa and reduction of Salmonella enteritidis colonization. The chemoattractant property of this chemokine will be evaluated in vitro, in a chemotaxis assay. Later, the chemokine will be vectorized by an attenuated strain of Salmonella Typhimurium in the intestinal lumen of chickens to assess the attraction of phagocytes and lymphocytes and the control of Salmonella enteritidis after challenge. Thus, this research project aims to evaluate new measures to prevent and control non-typhoidal Salmonella enterica serovars, pathogens of difficult control and high relevance to public health. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASARIN PENHA FILHO, RAFAEL ANTONIO; FERREIRA, JOSEANE CRISTINA; IBA KANASHIRO, ANA MARIA; JUNIOR, ANGELO BERCHIERI; DA COSTA DARINI, ANA LUCIA. Emergent multidrug-resistant nontyphoidal Salmonella serovars isolated from poultry in Brazil coharboring bia(CTX-M-2) and qnrB or bla(CMY-2) in large plasmids. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 95, n. 1, p. 93-98, SEP 2019. Web of Science Citations: 0.
GALETTI, RENATA; CASARIN PENHA FILHO, RAFAEL ANTONIO; FERREIRA, JOSEANE CRISTINA; VARANI, ALESSANDRO M.; COSTA DARINI, ANA LIICIA. Antibiotic resistance and heavy metal tolerance plasmids: the antimicrobial bulletproof properties of Escherichia fergusonii isolated from poultry. INFECTION AND DRUG RESISTANCE, v. 12, p. 1029-1033, 2019. Web of Science Citations: 1.
FERREIRA, JOSEANE CRISTINA; CASARIN PENHA FILHO, RAFAEL ANTONIO; YORIKA KUAYE, ANA PAULA; ANDRADE, LEONARDO NEVES; BERCHIERI JUNIOR, ANGELO; DA COSTA DARINI, ANA LUCIA. Identification and characterization of plasmid-mediated quinolone resistance determinants in Enterobacteriaceae isolated from healthy poultry in Brazil. INFECTION GENETICS AND EVOLUTION, v. 60, p. 66-70, JUN 2018. Web of Science Citations: 8.

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