Silk fibroin hydroxyapatite composite thermal stabilisation of carbonic anhydrase

Autor(es)

Lopes, J. H. ^[1] ; Guilhou, M. ^[1] ; Marelli, B. ^[2] ; Omenetto, F. G. ^[2] ; Kaplan, D. L. ^[2] ; Barralet, J. E. ^{[1, 3]} ; Merle, G. ^[1]

Afiliação do(s) autor(es)

^[1] McGill Univ, Fac Dent, Montreal, PQ H3A 2B2 - Canada

^[2] Tufts Univ, Dept Biomed Engn, Medford, MA 02155 - USA

^[3] McGill Univ, Fac Med, Dept Surg, Montreal, PQ H3A 2B2 - Canada

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF MATERIALS CHEMISTRY A; v. 3, n. 38, p. 19282-19287, 2015.
• Citações Web of Science: 7

Resumo

Carbonic anhydrase was entrapped in a matrix of ultrasonically bonded hydroxyapatite microparticles coated with beta-sheet structured silk fibroin. Transfer of the reactant and product between the enzyme and the assembly surface was evident and the system showed a remarkable operational, storage and thermal stability, with enzymatic activity almost unchanged after a one hour's treatment at 110 degrees C and the assembly retained 45% of its initial activity after 3 weeks of continuous heating at 80 degrees C in an amine solution. This thermal stability was excellent compared with described CA immobilization systems and indicates that silk fibroin may limit thermally induced enzyme conformation changes and prevent desorption. (AU)

• Processo FAPESP: 13/12376-5 - Formação óssea controlada por íons inorgânicos biologicamente ativos: investigação do papel e entrega controlada de íons relevantes a partir de biomateriais
• Beneficiário: João Henrique Lopes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado