In-Vitro Antiviral Activities of Extracts of Plants of The Brazilian Cerrado against the Avian Metapneumovirus (aMPV)

Autor(es)

Kohn, L. K. ^{[1, 2]} ; Foglio, M. A. ^[3] ; Rodrigues, R. A. ^[3] ; Sousa, I. M. de O. ^[3] ; Martini, M. C. ^[1] ; Padilla, M. A. ^[1] ; de Lima Neto, D. F. ^[1] ; Arns, C. W. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas Unicamp, Inst Biol, Virol Lab, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Francisco, BR-13045510 Campinas, SP - Brazil

^[3] Univ Campinas Unicamp, Inst Chem, Lab Res & Dev Nat Prod, BR-13083970 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Poultry Science; v. 17, n. 3, p. 275-280, JUL-SEP 2015.
• Citações Web of Science: 3

Resumo

ABSTRACTAvian metapneumovirus (aMPV) is a negative-sense single-stranded RNA enveloped virus of the Metapneumovirus genus belonging to theParamyxoviridae family. This virus may cause significant economic losses to the poultry industry, despite vaccination, which is the main tool for controlling and preventing aMPV. The aim of this study was to evaluate the antiviral activity of extracts of four different native plants of the Brazilian Cerrado against aMPV. The antiviral activity against aMPV was determined by titration. This technique measures the ability of plant extract dilutions (25 to 2.5 µg mL-1) to inhibit the cytopathic effect (CPE) of the virus, expressed as inhibition percentage (IP). The maximum nontoxic concentration (MNTC) of the extracts used in antiviral assay was 25 µg mL-1for Aspidosperma tomentosumand Gaylussacia brasiliensis, and 2.5 µg mL-1for Arrabidaea chicaand Virola sebifera. Twelve different extracts derived from four plant species collected from the Brazilian Cerrado were screened for antiviral activity against aMPV. G. brasiliensis, A. chica,and V. sebifera extracts presented inhibition rates of 99% in the early viral replication stages, suggesting that these extracts act during the adsorption phase. On the other hand, A. tomentosum inhibited 99% virus replication after the virus entered the cell. The biomonitored fractioning of extracts active against aMPV may be a tool to identify the active compounds of plant extracts and to determine their precise mode of action. (AU)

• Processo FAPESP: 08/00331-9 - Utilização de coculturas microbianas para a produção e descoberta de produtos naturais bioativos
• Beneficiário: Miriam Harumi Kossuga
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/00700-4 - Implantação e triagem da atividade antiviral de extratos de plantas brasileiras frente a vírus animais
• Beneficiário: Clarice Weis Arns
• Modalidade de apoio: Auxílio à Pesquisa - Regular