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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Development and validation of a liquid chromatography method for anthocyanins in strawberry (Fragaria spp.) and complementary studies on stability, kinetics and antioxidant power

Texto completo
Autor(es):
Canuto, Gisele A. B. [1] ; Oliveira, Daniel R. [1] ; da Conceicao, Lucas S. M. [1] ; Farah, Joao P. S. [1] ; Tavares, Marina F. M. [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Chem, BR-05508900 Sao Paulo, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: Food Chemistry; v. 192, p. 566-574, FEB 1 2016.
Citações Web of Science: 15
Resumo

A RPLC-DAD method for the analysis of eight anthocyanins was developed, validated and applied to strawberry extracts. The chromatographic method was conducted under gradient elution in acidulated water-methanol mobile phase and octadecyl-silica columns. An ultrasound extraction procedure was optimized by a 3(2) factorial design (%liCI in methanol, temperature, and time) and response surface methodology. Method validation was performed according to the following parameters: linearity (R-2 >0.99, p-value < 10(-4), F> 725), LOD (3-7 mu mol L-1) and LOQ (9-22 gmol L-1), selectivity/specificity (baseline separation of all analytes and peak purity), instrumental precision (<6.4 %CV), repeatability (<6.3 %CV) and intermediate precision (<9.9 %CV), recovery (83-99%), robustness (mobile phase pH, coluirm temperature and flow rate) and stability (high temperatures and storage; 1st order kinetics). The antioxidant power of anthocyanins was measured on-line (ABTS{''}+ reaction; Trolox as reference). Ten strawberry extracts were quantified (average values: 24.2 mu g/g for cyanidin-3-glucoside and 49.1 mu g/g for pelargonidin-3-glucoside). (C) 2015 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 98/13437-6 - Caracterização cromatográfica de extratos vegetais de uso cosmetológico
Beneficiário:Marina Franco Maggi Tavares
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 12/07361-6 - Modulação de mobilidade em eletroforese capilar
Beneficiário:Marina Franco Maggi Tavares
Linha de fomento: Auxílio à Pesquisa - Regular