Superoxide anion-induced pain and inflammation depends on TNF alpha/TNFR1 signaling in mice

Autor(es)

Yamacita-Borin, Fabiane Y. ^{[1, 2]} ; Zarpelon, Ana C. ^[1] ; Pinho-Ribeiro, Felipe A. ^[1] ; Fattori, Victor ^[1] ; Alves-Filho, Jose C. ^[3] ; Cunha, Fernando Q. ^[3] ; Cunha, Thiago M. ^[3] ; Casagrande, Rubia ^[2] ; Verri, Jr., Waldiceu A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Londrina, Ctr Ciencias Biol, Dept Ciencias Patol, BR-86057970 Londrina, Parana - Brazil

^[2] Univ Estadual Londrina, Dept Ciencias Farmaceut, Ctr Ciencias Saude, BR-86038350 Londrina, Parana - Brazil

^[3] Univ Sao Paulo, Dept Pharmacol, Ribeirao Preto Med Sch, BR-14049900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Neuroscience Letters; v. 605, p. 53-58, SEP 25 2015.
• Citações Web of Science: 23

Resumo

Inhibition of tumor necrosis factor-alpha (TNF alpha) and superoxide anion production reduces inflammation and pain. The present study investigated whether superoxide anion-induced pain depends on TNF alpha signaling and the role of superoxide anion in TNF alpha-induced hyperalgesia to clarify the interrelation between these two mediators in the context of pain. Intraplantar injection of a superoxide anion donor (potassium superoxide) induced mechanical hyperalgesia (0.5-5 h after injection), neutrophil recruitment (myeloperoxidase activity), and overt pain-like behaviors (paw flinching, paw licking, and abdominal writhings) in wild-type mice. Tumor necrosis factor receptor 1 deficiency (TNFR1-/-) and treatment of wild-type mice with etanercept (a soluble TNFR2 receptor that inhibits TNF alpha actions) inhibited superoxide anion-induced pain-like behaviors. TNFR1-/- mice were also protected from superoxide anion donor-induced oxidative stress, suggesting the role of this pathway in the maintenance of oxidative stress. Finally, we demonstrated that Apocynin (an NADPH oxidase inhibitor) or Tempol (a superoxide dismutase mimetic) treatment inhibited TNFa-induced paw mechanical hyperalgesia and neutrophil recruitment (myeloperoxidase activity). These results demonstrate that TNF alpha/TNFR1 signaling is important in superoxide anion-triggered pain and that TNF alpha/TNFR1 signaling amplifies the oxidative stress triggered by superoxide anion, which contributes to sustaining pain and inflammation. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 11/19670-0 - Mecanismos envolvidos na fisiopatologia da artrite reumatóide, dor e sepse
• Beneficiário: Fernando de Queiroz Cunha
• Modalidade de apoio: Auxílio à Pesquisa - Temático