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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Dichlorinated and Brominated Rugulovasines, Ergot Alkaloids Produced by Talaromyces wortmannii

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Autor(es):
de Medeiros, Livia Soman [1, 2] ; da Silva, Jose Vinicius [1] ; Abreu, Lucas Magalhaes [2, 3] ; Pfenning, Ludwig Heinrich [3] ; Silva, Carolina Lucia [1] ; Thomasi, Sergio Secherrer [1] ; Venancio, Tiago [1] ; van Pee, Karl-Heinz [4] ; Nielsen, Kristian Fog [2] ; Rodrigues-Filho, Edson [1]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Fed Sao Carlos, Dept Chem, BR-13565905 Sao Carlos, SP - Brazil
[2] Tech Univ Denmark, Dept Syst Biol, DK-2800 Lyngby - Denmark
[3] Univ Fed Lavras, Dept Phytopathol, BR-37200000 Lavras - Brazil
[4] Tech Univ Dresden, Allgemeine Biochem, D-01062 Dresden - Germany
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: Molecules; v. 20, n. 9, p. 17627-17644, SEP 2015.
Citações Web of Science: 3
Resumo

UHPLC-DAD-HRMS based dereplication guided the detection of new halogenated alkaloids co-produced by Talaromyces wortmannii. From the fungal growth in large scale, the epimers 2,8-dichlororugulovasines A and B were purified and further identified by means of a HPLC-SPE/NMR hyphenated system. Brominated rugulovasines were also detected when the microbial incubation medium was supplemented with bromine sources. Studies from 1D/2D NMR and HRMS spectroscopy data allowed the structural elucidation of the dichlorinated compounds, while tandem MS/HRMS data analysis supported the rationalization of brominated congeners. Preliminary genetic studies revealed evidence that FADH(2) dependent halogenase can be involved in the biosynthesis of the produced halocompounds. (AU)

Processo FAPESP: 09/18584-3 - Prospecção química do metabolismo secundário de fungos endofíticos isolados de maçã (Malus domestica) e goiaba (Psidium guajava)
Beneficiário:Lívia Soman de Medeiros
Modalidade de apoio: Bolsas no Brasil - Doutorado