Preparation and characterization of a microencapsulated polyethylene glycol cross-linked polyhemoglobin

Autor(es)

Knirsch, Marcos C. ^{[1, 2]} ; Dell'Anno, Filippo ^[3] ; Chicoma, Dennis ^[4] ; Stephano, Marco Antonio ^[2] ; Bou-Chacra, Nadia A. ^[5] ; Palombo, Domenico ^{[6, 7]} ; Converti, Attilio ^[3] ; Polakiewicz, Bronislaw ^[2]

Afiliação do(s) autor(es)

^[1] Minist Educ Brazil, CAPES Fdn, BR-70040020 Brasilia, DF - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo, SP - Brazil

^[3] Univ Genoa, Polytech Sch, Dept Civil Chem & Environm Engn, Pole Chem Engn, I-16145 Genoa - Italy

^[4] Univ Sao Paulo, Dept Chem Engn, Polytech Sch, BR-05508000 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Dept Pharm, Fac Pharmaceut Sci, BR-05508000 Sao Paulo, SP - Brazil

^[6] Univ Genoa, Vasc & Endovasc Surg Unit, Res Lab Expt & Clin Vasc Biol, DISC, I-16132 Genoa - Italy

^[7] IRCCS San Martino Hosp, I-16132 Genoa - Italy

• Tipo de documento: Artigo Científico
• Fonte: Bioprocess and Biosystems Engineering; v. 38, n. 11, p. 2263-2269, NOV 2015.
• Citações Web of Science: 2

Resumo

Many complications are associated to the therapeutic use of blood, among which are not only transfusion adverse events but also other issues such as lack of donors and high costs for collecting, testing, preserving, and distributing blood packages. Therefore, a clinically viable ``blood substitute{''} is considered the holy grail of traumatology and may greatly benefit medicine. One of the most successful approaches to date is conjugating hemoglobin with polyethylene glycol (PEG). This conjugation aims mainly at overcoming free cell hemoglobin toxicity, which makes its use as oxygen carrier in pure form unfeasible. To improve PEG-hemoglobin conjugates feasibility, we propose applying dual functional PEG cross-linking hemoglobin molecules encapsulated by a protein carrier. The new oxygen carrier showed mean values of the hydrodynamic diameter, dispersity, and zeta potential of 1370 nm, 0.029 and -36 mV, respectively, evidencing the successful synthesis of PEG bis(N-succinimidyl succinate) and polyhemoglobin as well as the structuring of protein carrier. (AU)

• Processo FAPESP: 11/21933-0 - Síntese e caracterização de derivados de hemoglobina para aplicação terapêutica
• Beneficiário: Marcos Camargo Knirsch
• Modalidade de apoio: Bolsas no Brasil - Doutorado