Biochemical and morphological alterations of the extracellular matrix of chicken calcaneal tendon during maturation

Autor(es)

Feo, Haline Ballestero ^[1] ; Biancalana, Adriano ^[2] ; Nakagaki, Wilson Romero ^[3] ; De Aro, Andrea Aparecida ^[1] ; Gomes, Laurecir ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Dept Funct & Struct Biol, IB, BR-13083865 Campinas, SP - Brazil

^[2] Fed Univ Para UFPA, Dept Cell Biol, Belem, Para - Brazil

^[3] Univ Western Sao Paulo UNOESTE, Presidente Prudente, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 78, n. 11, p. 949-957, NOV 2015.
• Citações Web of Science: 2

Resumo

The region in tendons that surrounds bone extremities adapts to compression forces, developing a fibrocartilaginous structure. During maturation, changes occur in the amount and organization of macromolecules of the extracellular matrix of tendons, changing the tissue morphology. To study the effect of maturation on tendons, Pedres chickens were sacrificed at 1, 5, and 8 months old and had the calcaneal tendon (CT) divided into proximal region, submitted to tension/compression forces (p), and distal region submitted to tension force (d). Morphological analysis of the p region showed the presence of fibrocartilage in all ages. In the central part of the fibrocartilage, near a diminishment of the metachromasy, there was also a development of a probable fat pad that increased with the maturation. The activity of MMP-2 and MMP-9 was higher at 5 and 8 months old, in both regions, compared with 1-month-old animals. In SDS-PAGE analysis, components with electrophoretic migration similar to decorin and fibromodulin increased with maturation, particularly in the d region. The Western blotting confirmed the increased amount of fibromodulin with maturation. In conclusion, our results show that process of maturation leads to the appearance of a probable fat pad in the center of the fibrocartilage of CT, with a reduced amount of glycosaminoglycans and an increased activity of MMPs. Microsc. Res. Tech. 78:949-957, 2015. (c) 2015 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 06/00044-4 - Morfologia e biomecânica do tendão gastrocnêmio de frangos em três idades diferentes
• Beneficiário: Laurecir Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular