Structural and functional stabilization of protein entities: state-of-the-art

Autor(es)

Balcao, Victor M. ^{[1, 2]} ; Vila, Marta M. D. C. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Minho, CEB Ctr Biol Engn, Braga - Portugal

^[2] Univ Sorocaba, I Intelligent Biosensing & Biomol Stabilizat Res, LaBNUS Biomat & Nanotechnol Lab, Sorocaba, SP - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: Advanced Drug Delivery Reviews; v. 93, p. 25-41, OCT 1 2015.
• Citações Web of Science: 87

Resumo

Within the context of biomedicine and pharmaceutical sciences, the issue of (therapeutic) protein stabilization assumes particular relevance. Stabilization of protein and protein-like molecules translates into preservation of both structure and functionality during storage and/or targeting, and such stabilization is mostly attained through establishment of a thermodynamic equilibrium with the (micro)environment The basic thermodynamic principles that govern protein structural transitions and the interactions of the protein molecule with its (micro)environment are, therefore, tackled in a systematic fashion. Highlights are given to the major classes of (bio)therapeutic molecules, viz, enzymes, recombinant proteins, (macro)peptides, (monoclonal) antibodies and bacteriophages. Modification of the microenvironment of the biomolecule via multipoint covalent attachment onto a solid surface followed by hydrophilic polymer co-immobilization, or physical containment within nanocarriers, are some of the (latest) strategies discussed aiming at full structural and functional stabilization of said biomolecules. (C) 2014 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 11/51077-8 - Vitor Manuel Cardoso Figueiredo Balcão | Universidade Fernando Pessoa - Portugal
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Pesquisador Visitante - Internacional
• Processo FAPESP: 13/03181-6 - PneumoPhageKill: desenvolvimento de sistema terapêutico contendo bacteriófagos nanoencapsulados líticos para Pseudomonas aeruginosa, para tratamento de pneumonia bacteriana por aerossolização
• Beneficiário: Marta Maria Duarte Carvalho Vila
• Modalidade de apoio: Auxílio à Pesquisa - Regular