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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Redox-active biflavonoids from Garcinia brasiliensis as inhibitors of neutrophil oxidative burst and human erythrocyte membrane damage

Texto completo
Autor(es):
Arwa, Phanuel Saroni [1] ; Zeraik, Maria Luiza [1] ; Ximenes, Valdecir Farias [2] ; da Fonseca, Luiz Marcos [3] ; Bolzani, Vanderlan da Silva [1] ; Siqueira Silva, Dulce Helena [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ UNESP, Nucleus Bioassays Ecophysiol & Biosynth Nat Prod, Dept Organ Chem, Inst Chem, BR-14800900 Sao Paulo - Brazil
[2] Sao Paulo State Univ UNESP, Dept Chem, Sch Sci, BR-17033360 Sao Paulo - Brazil
[3] Sao Paulo State Univ UNESP, Dept Clin Anal, Sch Pharmaceut Sci, BR-14801902 Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Ethnopharmacology; v. 174, p. 410-418, NOV 4 2015.
Citações Web of Science: 18
Resumo

Ethnopharmacological relevance: Garcinia brasiliensis, a plant native to the Brazilian Amazon Rainforest, is used in traditional medicine to treat inflammation of the urinary tract, peptic ulcers, arthritis and other conditions. Aim of the study: The purposes of this study were to analyze the chemical constituents of G. brasiliensis branches and leaves and to evaluate the potential of isolated compounds to act as inhibitors of both the oxidative burst of stimulated neutrophils and oxidative damage in human erythrocyte membranes to verify the antioxidant and anti-inflammatory effects of this plant. Materials and methods: Neutrophils were isolated from the blood of healthy donors by Ficoll-Paque density gradient centrifugation. Superoxide anion and total reactive oxygen species (ROS) produced by stimulated neutrophils were measured by WST-1 reduction and luminol-enhanced chemiluminescence assays, respectively. Radical-induced lipoperoxidation and hemolysis were performed using erythrocytes from the blood of healthy donors. Compounds were isolated from G. brasiliensis branches and leaves by HPLC microfractionation, and structure elucidation of the isolated compounds was performed based on NMR and HR-MS analyses. Results: The biflavonoids procyanidin, fukugetin, amentoflavone and podocarpusflavone isolated from G. brasiliensis showed potent inhibitory effects on the oxidative burst of human neutrophils, inhibiting ROS production by 50% at 1 mu mol L-1. These biflavonoids also proved to be potent inhibitors of hemolysis (with 88 +/- 7% inhibition at 50 mu mol L-1 for procyanidin) and lipid peroxidation in human erythrocytes, with a malondialdehyde level (a biomarker of oxidative stress) of 8.5 +/- 0.3 nmol/mg Hb at 50 mu mol L-1 for procyanidin. Conclusions: These findings indicate that the biflavonoids extracted from G. brasiliensis branches and leaves modulate oxidative stress via inhibition of NADPH oxidase and ROS production by stimulated human neutrophils. Furthermore, the biflavonoids exhibited potent inhibition of oxidant hemolysis and lipid peroxidation induced by AAPH in human erythrocytes. Therefore, these studies suggest the use of G. brasiliensis extract as an antioxidant and anti-inflammatory agent. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

Processo FAPESP: 11/03017-6 - Produtos naturais oriundos de plantas do Cerrado e Mata Atlântica, modelos potenciais e úteis para identificar protótipos com ação oxidante em neutrófilos e enzima mieloperoxidase (MPO)
Beneficiário:Maria Luiza Zeraik
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 10/52327-5 - Prospecção de moléculas bioativas e estudo de variabilidade infra-específica em plantas e microorganismos endófitos do cerrado e caatinga: contribuição para o conhecimento e uso sustentável da biodiversidade brasileira
Beneficiário:Vanderlan da Silva Bolzani
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Regular