Redox-active biflavonoids from Garcinia brasiliensis as inhibitors of neutrophil oxidative burst and human erythrocyte membrane damage

Author(s)

Arwa, Phanuel Saroni ^[1] ; Zeraik, Maria Luiza ^[1] ; Ximenes, Valdecir Farias ^[2] ; da Fonseca, Luiz Marcos ^[3] ; Bolzani, Vanderlan da Silva ^[1] ; Siqueira Silva, Dulce Helena ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Nucleus Bioassays Ecophysiol & Biosynth Nat Prod, Dept Organ Chem, Inst Chem, BR-14800900 Sao Paulo - Brazil

^[2] Sao Paulo State Univ UNESP, Dept Chem, Sch Sci, BR-17033360 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Clin Anal, Sch Pharmaceut Sci, BR-14801902 Sao Paulo - Brazil

• Document type: Journal article
• Source: Journal of Ethnopharmacology; v. 174, p. 410-418, NOV 4 2015.
• Web of Science Citations: 16

Abstract

Ethnopharmacological relevance: Garcinia brasiliensis, a plant native to the Brazilian Amazon Rainforest, is used in traditional medicine to treat inflammation of the urinary tract, peptic ulcers, arthritis and other conditions. Aim of the study: The purposes of this study were to analyze the chemical constituents of G. brasiliensis branches and leaves and to evaluate the potential of isolated compounds to act as inhibitors of both the oxidative burst of stimulated neutrophils and oxidative damage in human erythrocyte membranes to verify the antioxidant and anti-inflammatory effects of this plant. Materials and methods: Neutrophils were isolated from the blood of healthy donors by Ficoll-Paque density gradient centrifugation. Superoxide anion and total reactive oxygen species (ROS) produced by stimulated neutrophils were measured by WST-1 reduction and luminol-enhanced chemiluminescence assays, respectively. Radical-induced lipoperoxidation and hemolysis were performed using erythrocytes from the blood of healthy donors. Compounds were isolated from G. brasiliensis branches and leaves by HPLC microfractionation, and structure elucidation of the isolated compounds was performed based on NMR and HR-MS analyses. Results: The biflavonoids procyanidin, fukugetin, amentoflavone and podocarpusflavone isolated from G. brasiliensis showed potent inhibitory effects on the oxidative burst of human neutrophils, inhibiting ROS production by 50% at 1 mu mol L-1. These biflavonoids also proved to be potent inhibitors of hemolysis (with 88 +/- 7% inhibition at 50 mu mol L-1 for procyanidin) and lipid peroxidation in human erythrocytes, with a malondialdehyde level (a biomarker of oxidative stress) of 8.5 +/- 0.3 nmol/mg Hb at 50 mu mol L-1 for procyanidin. Conclusions: These findings indicate that the biflavonoids extracted from G. brasiliensis branches and leaves modulate oxidative stress via inhibition of NADPH oxidase and ROS production by stimulated human neutrophils. Furthermore, the biflavonoids exhibited potent inhibition of oxidant hemolysis and lipid peroxidation induced by AAPH in human erythrocytes. Therefore, these studies suggest the use of G. brasiliensis extract as an antioxidant and anti-inflammatory agent. (C) 2015 Elsevier Ireland Ltd. All rights reserved. (AU)

• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 11/03017-6 - Natural products from plants of Cerrado and Atlantic Forest, potential models and useful for identifying prototypes with oxidizing action on neutrophils and myeloperoxidase (MPO) enzyme
• Grantee: Maria Luiza Zeraik
• Support Opportunities: Scholarships in Brazil - Post-Doctoral