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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation

Texto completo
Autor(es):
Marthos, Bruno Vitorio [1] ; Silva Ferri, Anne Leticia [1] ; de Figueiredo, Douglas Borges [1] ; Zangirolami, Teresa Cristina [2] ; Goncalves, Viviane Maimoni [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Inst Butantan, Ctr Biotecnol, Lab Bioproc, BR-05503900 Sao Paulo, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Applied Microbiology and Biotechnology; v. 99, n. 24, p. 10447-10456, DEC 2015.
Citações Web of Science: 1
Resumo

Streptococcus pneumoniae is a human pathogen largely transmitted by aerosols. Vaccines are the main strategy against this pathogen, and the capsular polysaccharide (PS) is its major antigen. S. pneumoniae serotype 1 is associated with large outbreaks and epidemics of invasive diseases. The aims of this work were to screen serotype 1 strains to identify the best PS1 producer, evaluate three peptones for PS1 production, investigate the effects of culture medium components using a design of experiments (DoE), a statistic tool for optimization, and propose a new medium/cultivation strategy. After flask cultivation of nine strains, two that produced high PS1 and biomass values were chosen for further evaluation in the bioreactor, and ST595/01 was chosen as the best PS1 producer strain. Among the peptones tested (Casamino acids, Soytone, and Phytone), the highest PS1 production (298 mg/L) was reached with Phytone. Next, DoE (2(4-1)) was performed to evaluate the effects of yeast extract (YE), Phytone, L-asparagine (Asn), and L-glutamine (Gln), yielding the following results: Phytone presented positive effects (p < 0.05) for maximum production of biomass, PS1, acetate, and lactate; YE showed positive effects for biomass and acid production (p < 0.05); Gln exerted a minor positive effect on PS1 yield factor on glucose (p < 0.1); and Asn presented only an effect on acetate production (p < 0.1). Hence, a new culture medium was formulated based on Phytone, YE, and glucose, and batch and fed-batch cultivations were evaluated. The fed-batch cultivation showed almost 2 times the biomass and 2.5 times the PS1 production as the batch culture, and 8-10 times higher PS1 production than has been previously reported. (AU)

Processo FAPESP: 11/01262-3 - Determinação da influência do meio de armazenamento sobre a viabilidade celular e a produção de polissacarídeo capsular de Streptococcus pneumoniae
Beneficiário:Douglas Borges de Figueiredo
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 08/05207-4 - Vacina conjugada anti-pneumocócica: estudos sobre a viabilidade de uma vacina polissacarídeo capsular - PSPA
Beneficiário:Martha Massako Tanizaki
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 11/02805-0 - Determinação dos aminoácidos essenciais e da influência das bases nitrogenadas em cultivo contínuo de Streptococcus pneumoniae sorotipo 14
Beneficiário:Anne Leticia Silva Ferri
Modalidade de apoio: Bolsas no Brasil - Mestrado
Processo FAPESP: 08/10364-1 - Impacto das estratégias de cultivo de Streptococcus pneumoniae sobre a produção do polissacarídeo capsular
Beneficiário:Viviane Maimoni Gonçalves
Modalidade de apoio: Auxílio à Pesquisa - Regular