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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation

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Marthos, Bruno Vitorio [1] ; Silva Ferri, Anne Leticia [1] ; de Figueiredo, Douglas Borges [1] ; Zangirolami, Teresa Cristina [2] ; Goncalves, Viviane Maimoni [1]
Total Authors: 5
[1] Inst Butantan, Ctr Biotecnol, Lab Bioproc, BR-05503900 Sao Paulo, SP - Brazil
[2] Univ Fed Sao Carlos, Dept Engn Quim, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Applied Microbiology and Biotechnology; v. 99, n. 24, p. 10447-10456, DEC 2015.
Web of Science Citations: 1

Streptococcus pneumoniae is a human pathogen largely transmitted by aerosols. Vaccines are the main strategy against this pathogen, and the capsular polysaccharide (PS) is its major antigen. S. pneumoniae serotype 1 is associated with large outbreaks and epidemics of invasive diseases. The aims of this work were to screen serotype 1 strains to identify the best PS1 producer, evaluate three peptones for PS1 production, investigate the effects of culture medium components using a design of experiments (DoE), a statistic tool for optimization, and propose a new medium/cultivation strategy. After flask cultivation of nine strains, two that produced high PS1 and biomass values were chosen for further evaluation in the bioreactor, and ST595/01 was chosen as the best PS1 producer strain. Among the peptones tested (Casamino acids, Soytone, and Phytone), the highest PS1 production (298 mg/L) was reached with Phytone. Next, DoE (2(4-1)) was performed to evaluate the effects of yeast extract (YE), Phytone, L-asparagine (Asn), and L-glutamine (Gln), yielding the following results: Phytone presented positive effects (p < 0.05) for maximum production of biomass, PS1, acetate, and lactate; YE showed positive effects for biomass and acid production (p < 0.05); Gln exerted a minor positive effect on PS1 yield factor on glucose (p < 0.1); and Asn presented only an effect on acetate production (p < 0.1). Hence, a new culture medium was formulated based on Phytone, YE, and glucose, and batch and fed-batch cultivations were evaluated. The fed-batch cultivation showed almost 2 times the biomass and 2.5 times the PS1 production as the batch culture, and 8-10 times higher PS1 production than has been previously reported. (AU)

FAPESP's process: 08/05207-4 - Pneumococcal conjugate vaccine: capsular polysaccharide - pneumococcal surface protein A
Grantee:Martha Massako Tanizaki
Support type: Research Projects - Thematic Grants
FAPESP's process: 08/10364-1 - Impact of Streptococcus pneumoniae cultivation strategies on capsular polysaccharide production
Grantee:Viviane Maimoni Gonçalves
Support type: Regular Research Grants
FAPESP's process: 11/01262-3 - Influence of the storage medium on cell viability and capsular polysaccharide production by Streptococcus pneumoniae
Grantee:Douglas Borges de Figueiredo
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 11/02805-0 - Determination of essential amino acids and influence of nitrogenous bases on the continuous cultivation of Streptococcus pneumoniae serotype 14
Grantee:Anne Leticia Silva Ferri
Support type: Scholarships in Brazil - Master