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Pneumococcal conjugate vaccine: capsular polysaccharide - pneumococcal surface protein A

Grant number: 08/05207-4
Support type:Research Projects - Thematic Grants
Duration: April 01, 2009 - June 30, 2014
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Martha Massako Tanizaki
Grantee:Martha Massako Tanizaki
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Co-Principal Investigators:Luciana Cezar de Cerqueira Leite
Assoc. researchers:Joaquin Cabrera-Crespo ; Jose Geraldo da Cruz Pradella ; Luciana Cezar de Cerqueira Leite ; Mickie Takagi ; Roberto de Campos Giordano ; Viviane Maimoni Gonçalves
Associated grant(s):10/15479-1 - Advanced computational intelligence (CI) techniques applied to the production of anti-pneumococcal conjugate vaccine, AV.BR
Associated scholarship(s):14/11557-9 - Monitoring of morphology and rheology during recombinant Escherichia coli cultures, BP.IC
13/20985-1 - Characterization of Morphology and Recombinant Protein Production of E. coli Cultures at Different Induction Conditions, BP.IC
12/22250-6 - Evaluation of cross-reactivity between anti-PspA antibodies and the S2 subfragment of human cardiac myosin, BP.IC
+ associated scholarships 12/13015-3 - Identification of glycosylation sites of S. pneumoniae capsular polysaccharide-PspA conjugates by mass spectrometry, BE.PQ
12/04858-7 - Development of the purification process of the pneumococcal surface protein a from Clade4 (PspA4pro), BP.MS
12/04286-3 - The study of polyamines transporter, PotD, as a vaccine antigen against Streptococcus pneumoniae, BP.DD
11/16605-3 - Cultivation of rE. coli in airlift bioreactor: development of strategies for optimization of biomass production, BP.MS
11/02805-0 - Determination of essential amino acids and influence of nitrogenous bases on the continuous cultivation of Streptococcus pneumoniae serotype 14, BP.MS
11/01262-3 - Influence of the storage medium on cell viability and capsular polysaccharide production by Streptococcus pneumoniae, BP.IC
10/17047-1 - Study of the influence of aminoacids additon on the PspA protein expression by rE. coli, BP.IC
10/07916-2 - On line estimation of kinetic parameters during high density cultivations of recombinant E. coli expressing a pneumococcus A surface protein, BP.IC
09/17030-4 - Pneumococcal protein vaccines, evaluation of immune responses under different presentations, BP.DD
08/07190-1 - Optimization of polysaccharide production of Streptococcus pneumoniae serotype 6B in bioreactor, BP.DD
08/06907-0 - Antipneumococcal Conjugate Vaccine PS6B-PspA, BP.MS - associated scholarships


Streptococcus pneumoniae (pneumococcus) is a pathogenic, encapsulated bacteria. The capsules from these bacteria are constituted of a large chain polysaccharide (PS) and their different structures are related to a particular serotype. There are 90 different serotypes described in S. pneumoniae. Vaccines against pneumococcus are constituted by the purified PS, plain or conjugated to a protein. The advantage of a conjugated vaccine is to change the PS antigen from a thymus independent to thymus dependent one. The process for conjugate vaccine production is always very expensive particularly the conjugation step due to low recovery of conjugate. This is a special problem for pneumococcal vaccines which should include over 7 serotypes, which vary with geographical region. A variety of surface proteins have been investigated as alternative vaccine antigens, and pneumococcal surface protein A, PspA, has shown the most promising results. The present project intends to use PspA as carrier protein for PSs and antigen, aiming at extending the vaccine coverage using few conjugates. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, MIRIAM A.; CONVERSO, THIAGO R.; GONCALVES, VIVIANE M.; LEITE, LUCIANA C. C.; TANIZAKI, MARTHA M.; BARAZZONE, GIOVANA C. Conjugation of PspA4Pro with Capsular Streptococcus pneumoniae Polysaccharide Serotype 14 Does Not Reduce the Induction of Cross-Reactive Antibodies. Clinical and Vaccine Immunology, v. 24, n. 8 AUG 2017. Web of Science Citations: 2.
R. T. ZANARDO; A. L. S. FERRI; D. B. FIGUEIREDO; S. KRASCHOWETZ; J. CABRERA-CRESPO; V. M. GONÇALVES. DEVELOPMENT OF A NEW PROCESS FOR PURIFICATION OF CAPSULAR POLYSACCHARIDE FROM Streptococcus pneumoniae SEROTYPE 14. Brazilian Journal of Chemical Engineering, v. 33, n. 3, p. 435-443, Set. 2016.
MARTHOS, BRUNO VITORIO; SILVA FERRI, ANNE LETICIA; DE FIGUEIREDO, DOUGLAS BORGES; ZANGIROLAMI, TERESA CRISTINA; GONCALVES, VIVIANE MAIMONI. Capsular polysaccharide production by Streptococcus pneumoniae serotype 1: from strain selection to fed-batch cultivation. Applied Microbiology and Biotechnology, v. 99, n. 24, p. 10447-10456, DEC 2015. Web of Science Citations: 1.
CAMPANI, GILSON; ARRUDA RIBEIRO, MARCELO PERENCIN; LUPERNI HORTA, ANTONIO CARLOS; GIORDANO, ROBERTO CAMPOS; BADINO, ALBERTO COLLI; ZANGIROLAMI, TERESA CRISTINA. Oxygen transfer in a pressurized airlift bioreactor. Bioprocess and Biosystems Engineering, v. 38, n. 8, p. 1559-1567, AUG 2015. Web of Science Citations: 8.
RODEGE GOGOLA-KOLLING, VERONICA MARIA; ZANARDO, RAFAELA TAIS; CARMO, TALITA SOUZA; ZAMPOLI, NATALIA DALFRE; FIGUEIREDO, DOUGLAS BORGES; GONCALVES, VIVIANE MAIMONI. Improved capsular polysaccharide production by Streptococcus pneumoniae serotype 14 using continuous cultivation. Biochemical Engineering Journal, v. 91, p. 16-22, OCT 15 2014. Web of Science Citations: 3.
BARAZZONE, GIOVANA CAPPIO; PINTO, VITTORIA; DONNARUMMA, DANILO; TANIZAKI, MARTHA MASSAKO; NORAIS, NATHALIE; BERTI, FRANCESCO. Identification of glycosylated regions in pneumococcal PspA conjugated to serotype 6B capsular polysaccharide. GLYCOCONJUGATE JOURNAL, v. 31, n. 3, p. 259-269, APR 2014. Web of Science Citations: 2.

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