Recombinant K28 antigen in ELISA in the diagnosis of canine visceral leishmaniosis

Autor(es)

Venturin, G. L. ^[1] ; Bragato, J. P. ^[1] ; Silva, K. L. O. ^[1] ; de Lima, V. M. F. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Fac Vet Med, Anim Sci, BR-16050680 Aracatuba, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Fac Vet Med, Dept Surg & Anim Reprod, BR-16050680 Aracatuba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PARASITE IMMUNOLOGY; v. 37, n. 12, p. 670-673, DEC 2015.
• Citações Web of Science: 4

Resumo

Crude total antigen (CTA) from Leishmania infantum and recombinant antigen K39 (rK39) and recombinant antigen K28 (rK28) were compared using an ELISA for the diagnosis of canine visceral leishmaniosis (CVL). Forty-two blood samples from healthy dogs from a nonendemic area and 80 blood samples from an endemic area for dogs with visceral leishmaniosis (VL), confirmed with positive parasitological tests for Leishmania spp., were used in an ELISA. The parasitological diagnosis was chosen as a gold standard. The ELISA with rK28 antigen showed sensitivity of 100% and specificity of 100%, high agreement with CTA and rK39, indicating that the rK28 antigen is useful for ELISA serological diagnosis of CVL. (AU)

• Processo FAPESP: 12/19822-8 - Comparação dos antígenos recombinantes K39 e K28 no diagnóstico sorológico da leishmaniose visceral canina pelo método ELISA
• Beneficiário: Jaqueline Poleto Bragato
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica