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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recombinant K28 antigen in ELISA in the diagnosis of canine visceral leishmaniosis

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Author(s):
Venturin, G. L. [1] ; Bragato, J. P. [1] ; Silva, K. L. O. [1] ; de Lima, V. M. F. [2]
Total Authors: 4
Affiliation:
[1] Sao Paulo State Univ UNESP, Fac Vet Med, Anim Sci, BR-16050680 Aracatuba, SP - Brazil
[2] Sao Paulo State Univ UNESP, Fac Vet Med, Dept Surg & Anim Reprod, BR-16050680 Aracatuba, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PARASITE IMMUNOLOGY; v. 37, n. 12, p. 670-673, DEC 2015.
Web of Science Citations: 4
Abstract

Crude total antigen (CTA) from Leishmania infantum and recombinant antigen K39 (rK39) and recombinant antigen K28 (rK28) were compared using an ELISA for the diagnosis of canine visceral leishmaniosis (CVL). Forty-two blood samples from healthy dogs from a nonendemic area and 80 blood samples from an endemic area for dogs with visceral leishmaniosis (VL), confirmed with positive parasitological tests for Leishmania spp., were used in an ELISA. The parasitological diagnosis was chosen as a gold standard. The ELISA with rK28 antigen showed sensitivity of 100% and specificity of 100%, high agreement with CTA and rK39, indicating that the rK28 antigen is useful for ELISA serological diagnosis of CVL. (AU)

FAPESP's process: 12/19822-8 - Recombinants antigens K39 and K28 in the diagnois of canine visceral leishmaniasis by ELISA assay
Grantee:Jaqueline Poleto Bragato
Support Opportunities: Scholarships in Brazil - Scientific Initiation