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Validation of Enzyme-Linked Immunosorbent Assay (ELISA) with recombinant antigens for the serological diagnosis of canine visceral leishmaniasis (VL)

Grant number: 15/22075-8
Support type:Regular Research Grants
Duration: March 01, 2016 - February 28, 2018
Field of knowledge:Biological Sciences - Immunology - Applied Immunology
Principal researcher:Hiro Goto
Grantee:Hiro Goto
Home Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers: Arleana Do Bom Parto Ferreira de Almeida ; José Eduardo Tolezano ; Maria Carmen Arroyo Sanchez ; Nazaré Fonseca de Souza ; Roberto Mitsuyoshi Hiramoto ; Valéria Régia Franco Sousa


In Brazil, visceral leishmaniasis (VL) is booming and present in all regions, with a prevalence of 3,253 cases in 2013, reaching also urban and peri-urban centers in the last three decades. It is caused by Leishmania (Leishmania) infantum. Sandfly Lutzomyia longipalpis is the main vector, and the main reservoirs, involved in domestic and wild cycle, respectively, are the dog (Canis familiaris) and fox (Dusycion vetulus). As dogs have an intense parasitism in the skin, it is believed that this will allow an easy vector infection constituting the important link in maintaining the epidemiological chain. Thus, the identification of infected dogs is considered a crucial measure in controlling the transmission of the disease. It is recommended to perform sample or census survey, depending on the epidemiological situation regarding the risk of transmission in the area being investigated. The Ministry of Health recommends conducting immunoassay DPP " Leishmania test for the screening of dogs and ELISA based on total lysate of Leishmania major-like for the confirmation of positive cases. Since the parasite culture for preparation of the total antigen constitutes a limiting factor, it would be desirable to have an ELISA based on the use of recombinant antigen, which we propose in this project. Thus, this study aims to validate the serological methods ELISA-rHsp83 and ELISA-rK28 for the diagnosis of canine VL replacing ELISA based on total lysate of Leishmania major-like as a confirmatory test after immunochromatographic DPP " Leishmania test. Validate alternatively serological methods ELISA-rHsp83 and ELISA-rK28 for the diagnosis of canine VL as a confirmatory test, regardless of the outcome of DPP " Leishmania test. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MAHYUMI FUJIMORI; ARLEANA DO BOM PARTO FERREIRA DE ALMEIDA; STELLA MARIA BARROUIN-MELO; LUIZ RICARDO PAES DE BARROS CORTEZ; MALCOLM SCOTT DUTHIE; ROBERTO MITSUYOSHI HIRAMOTO; FLAVIANE ALVES DE PINHO; STEVEN GREGORY REED; VALÉRIA RÉGIA FRANCO SOUSA; NAZARÉ FONSECA SOUZA; et al. Validation of ELISA with recombinant antigens in serological diagnosis of canine Leishmania infantum infection. Memórias do Instituto Oswaldo Cruz, v. 116, . (17/03367-3, 15/22075-8, 15/03117-1)
SATO, CAMILA MASSAE; ARROYO SANCHEZ, MARIA CARMEN; CELESTE, BEATRIZ JULIETA; DUTHIE, MALCOLM S.; GUDERIAN, JEFFREY; REED, STEVEN G.; FELINTO DE BRITO, MARIA EDILEUZA; CAMPOS, MARLIANE BATISTA; DE SOUZA ENCARNACAO, HELIA VALERIA; GUERRA, JORGE; et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495-503, . (15/22075-8)

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