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Recombinants antigens K39 and K28 in the diagnois of canine visceral leishmaniasis by ELISA assay

Grant number: 12/19822-8
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2012
Effective date (End): November 30, 2013
Field of knowledge:Biological Sciences - Immunology - Applied Immunology
Principal Investigator:Valéria Marçal Felix de Lima
Grantee:Jaqueline Poleto Bragato
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The Visceral Leishmaniasis (VL), also known as kala-azar, is a anthropozoonosis caused by protozoa of the genus Leishmania spp. that infect man and dog. VL is transmitted by the bite of female mosquitoes of the genus Phlebotomus and Lutzomyia. Domestic dogs (Canis familiaris), considered the main domestic reservoirs are of great importance in maintaining the disease cycle. Serologic tests to detect circulating antibodies are used as a tool in the diagnosis of clinical cases and in epidemiological surveys, there is a wide variety of techniques and antigens employed. Among the methods of diagnosis, parasitological examination for direct detection of parasites has high specificity, but sensitivity is low failing to detect cases where parasitism is low. The aim of this study is to compare the RK28 and rK39 antigens, with respect to sensitivity and specificity, by performing ELISA using sera from dogs naturally infected by Leishmania, confirmed by parasitological examination.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VENTURIN, G. L.; BRAGATO, J. P.; SILVA, K. L. O.; DE LIMA, V. M. F. Recombinant K28 antigen in ELISA in the diagnosis of canine visceral leishmaniosis. PARASITE IMMUNOLOGY, v. 37, n. 12, p. 670-673, DEC 2015. Web of Science Citations: 4.

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