|Support type:||Scholarships in Brazil - Scientific Initiation|
|Effective date (Start):||December 01, 2012|
|Effective date (End):||November 30, 2013|
|Field of knowledge:||Biological Sciences - Immunology - Applied Immunology|
|Principal Investigator:||Valéria Marçal Felix de Lima|
|Grantee:||Jaqueline Poleto Bragato|
|Home Institution:||Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil|
The Visceral Leishmaniasis (VL), also known as kala-azar, is a anthropozoonosis caused by protozoa of the genus Leishmania spp. that infect man and dog. VL is transmitted by the bite of female mosquitoes of the genus Phlebotomus and Lutzomyia. Domestic dogs (Canis familiaris), considered the main domestic reservoirs are of great importance in maintaining the disease cycle. Serologic tests to detect circulating antibodies are used as a tool in the diagnosis of clinical cases and in epidemiological surveys, there is a wide variety of techniques and antigens employed. Among the methods of diagnosis, parasitological examination for direct detection of parasites has high specificity, but sensitivity is low failing to detect cases where parasitism is low. The aim of this study is to compare the RK28 and rK39 antigens, with respect to sensitivity and specificity, by performing ELISA using sera from dogs naturally infected by Leishmania, confirmed by parasitological examination.