Evaluation of Monolithic and Core-Shell Columns for Separation of Triazine Herbicides by Reversed Phase High Performance Liquid Chromatography

Autor(es)

Urio, Ricardo P. ^[1] ; Masini, Jorge C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 26, n. 11, p. 2331-2338, NOV 2015.
• Citações Web of Science: 5

Resumo

Monolithic and core-shell columns were comparatively evaluated for separation of deisopropylatrazine (DIA), deethylatrazine (DEA), hydroxyatrazine (HAT), simazine (SIM) and atrazine (ATR) under similar conditions of elution, flow rate and sample volume. Although both columns provided separation of the analytes with resolution > 1.5, the core-shell column exhibited better efficiency, leading to narrower peaks. It has height equivalent to the theoretical plates (HETP) for DEA, DIA and HAT, eluted under isocratic elution, about 2.5 times smaller than those of the monolithic column. A soil sample was studied by ultrasound assisted extraction with 80:20 (v/v) acetonitrile:water, revealing the presence of HAT and ATR. This sample was enriched with 0.25, 0.50 and 1.0 mg kg-1 of the five triazine compounds and analyzed, leading to recovery percentages between 45 and 86%. (AU)

• Processo FAPESP: 13/18507-4 - Materiais monolíticos porosos para separações cromatográficas e preparo de amostras
• Beneficiário: Jorge Cesar Masini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/08309-5 - Desenvolvimento e avaliação de métodos de extração e separação cromatográfica para determinação de herbicidas triazínicos, uréias e uracilas em amostras de solos e águas
• Beneficiário: Ricardo de Prá Urio
• Modalidade de apoio: Bolsas no Brasil - Doutorado