Evaluation of Monolithic and Core-Shell Columns for Separation of Triazine Herbicides by Reversed Phase High Performance Liquid Chromatography

Author(s)

Urio, Ricardo P. ^[1] ; Masini, Jorge C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 26, n. 11, p. 2331-2338, NOV 2015.
• Web of Science Citations: 5

Abstract

Monolithic and core-shell columns were comparatively evaluated for separation of deisopropylatrazine (DIA), deethylatrazine (DEA), hydroxyatrazine (HAT), simazine (SIM) and atrazine (ATR) under similar conditions of elution, flow rate and sample volume. Although both columns provided separation of the analytes with resolution > 1.5, the core-shell column exhibited better efficiency, leading to narrower peaks. It has height equivalent to the theoretical plates (HETP) for DEA, DIA and HAT, eluted under isocratic elution, about 2.5 times smaller than those of the monolithic column. A soil sample was studied by ultrasound assisted extraction with 80:20 (v/v) acetonitrile:water, revealing the presence of HAT and ATR. This sample was enriched with 0.25, 0.50 and 1.0 mg kg-1 of the five triazine compounds and analyzed, leading to recovery percentages between 45 and 86%. (AU)

• FAPESP's process: 13/18507-4 - Porous monolithic materials for chromatographic separations and sample preparation
• Grantee: Jorge Cesar Masini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/08309-5 - Development and evaluation of extraction methods and chromatographic separation for determination of triazine, ureas and uracil herbicides in soil and water samples.
• Grantee: Ricardo de Prá Urio
• Support Opportunities: Scholarships in Brazil - Doctorate