Sequential Injection Chromatography (SIC) utilizes low-pressure chromatographic columns coupled to sequential injection systems. Separation capacity in SIC can be increased with some analytical strategies, including the development of new, more selective stationary phases available as monolithic columns. The introduction of the monolithic stationary phases was an advance in the technology of chromatographic columns, as it presented advantages such as high permeability, which culminated in high mobile phase flows under lower backpressures; wide variability of precursors available for the preparation of new stationary phases, simple preparation processes, and high mechanical resistance. Hydrophilic Interaction Chromatography (HILIC) is a combination of the normal phase, reverse phase and ion chromatography separation modes because it employs highly hydrophilic stationary phases typical of those used in normal mode such as non-derivatized silica or that chemically modified with polar groups, such as amino, cyano, diol or polyols. In addition, HILIC applies polar organic solvents and their mixtures, commonly used as mobile phases for reversed phase, and is capable of separating the target compounds by ion chromatography, such as ionized or ionizabled polar ones. The development of multidimensional separation systems is an alternative in SIC by exploring chromatographic dimensions based on different separation mechanisms. When the sample is subjected to this process, separation and distribution of the analytes are inherently improved, increasing resolution. For this reason, stationary phases for use in HILIC mode are usual in combination with traditional stationary phases for reversed phase mode in two-dimensional orthogonal chromatographic separations, because the incompatibility of mobile phases is avoided. In addition, polar analytes, which exhibit low reverse phase separation performance, can be successfully separated on hydrophilic stationary phases for HILIC.
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