Development and application of two-dimensional sequential injection chromatography

• Grant number: 14/19115-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2015
• End date: August 31, 2015
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Fábio Rodrigo Piovezani Rocha
• Grantee: Alex Domingues Batista
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

The introduction of sequential injection chromatography expanded the potentialities of flow analysis for determination of multicomponents. Strategies to improve selectivity and capacity of separation have been presented, as stepwise elution and fused-core particles columns. The analytical performance has also been improved by on-line sample treatment. The present project aims to expand the analytical capacity of SIC system by the use of two-dimensional chromatography applied to the determination of phenolic compounds in wine and the on-column preconcentration for determination of furfurals in fruit juices. The procedures will be based on two chromatographic columns with distinct properties. The analytes poorly separated in the first dimension will be processed in the second dimension, allowing the separation by a different type of interaction. Eight-ports selection valves will be used for solutions management and selection of the fractions from the first. On-column preconcentration will be based on the trapping of the analytes in the head of the chromatographic column for posterior separation by a suitable mobile phase. Theses procedures are innovative in sequential injection chromatography and will expand its separation capacity and applicability.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BATISTA, ALEX D.; AMAIS, RENATA S.; ROCHA, FABIO R. P.. Liquid-liquid microextraction in sequential injection analysis for the direct spectrophotometric determination of acid number in biodiesel. Microchemical Journal, v. 124, p. 55-59, JAN 2016. (13/26857-5, 14/19115-5)