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Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals

Grant number: 18/14582-5
Support type:Research Grants - Young Investigators Grants- Phase 2
Duration: May 01, 2019 - April 30, 2024
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal researcher:Maurício Ariel Rostagno
Grantee:Maurício Ariel Rostagno
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Assoc. researchers:Adriane Elisabete Antunes de Moraes ; Ana Carolina de Aguiar ; Augusto Ducati Luchessi ; Diogo Thimoteo da Cunha ; Fernando Moreira Simabuco ; Julian Martínez ; Rosângela Maria Neves Bezerra ; Tânia Forster Carneiro
Associated research grant:13/04304-4 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology, AP.JP
Associated grant(s):19/13496-0 - Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA), AP.EMU
Associated scholarship(s):20/15774-5 - Development of methods of extraction, separation and two-dimensional analysis of phenolic compounds in natural products, BP.PD
20/04067-6 - Evaluation of ultrasound-assisted pressurized fluid extraction coupled with solid phase extraction for extracting phenolic compounds and terpenes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis), BP.DD
20/08421-9 - Application of non-volatile alternative solvents to create sustainable two-dimensional and integrated platforms for harnessing agro-industrial waste from jaboticaba and pomegranate fruits, BP.PD
20/03623-2 - Extraction and separation of phenolic compounds from green propolis by a bidimensional system, BP.DD
19/24537-0 - Phytochemical evaluation of apple (Malus domestica) industrial residue from extraction and separation by a two-dimensional system, BP.DD


This research project proposes the development of a two-dimensional extraction, separation and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with gradient pressurized liquids (PLEgrad) with solid phase extraction (SPE) in the first dimension and orthogonally with ultra-high pressure chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and with real-time monitoring, while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. The system will be validated with several samples, including soybean (Glycine max), Brazilian ginseng (Pfaffia glomerata), black tea and green tea (Camellia sinensis), mate (Ilex paraguariensis), coffee (Coffea arabica), pomegranate granatum), orange peel (Citrus sinensis), sicilian lemon peel (Citrus x limon) apple peel (Malus domestica), jabuticaba bark (Myrciaria cauliflora), rosemary (Rosmarinus officinalis) and sage (Salvia officinalis). Initially the rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples <2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent and duration of the extraction / elution gradient. This proposal consists of a continuation of the Young Researcher project (FAPESP 2013 / 04304-4), seeking to consolidate this line of research at FCA / UNICAMP and to continue the training of qualified human resources. (AU)

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MACIEL-SILVA, FRANCISCO W.; BULLER, LUZ S.; GONCALVES, MARIA L. M. B. B.; ROSTAGNO, MAURICIO A.; FORSTER-CARNEIRO, TANIA. Sustainable development in the Legal Amazon: energy recovery from acai seeds. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, APR 2021. Web of Science Citations: 0.
OLIVEIRA, T. C. G.; HANLON, K. E.; INTERLANDI, M. A.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; LACHOS-PEREZ, D.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T. Subcritical water hydrolysis pretreatment of sugarcane bagasse to produce second generation ethanol. JOURNAL OF SUPERCRITICAL FLUIDS, v. 164, OCT 1 2020. Web of Science Citations: 0.
DA SILVA, LAISE C.; SOUZA, MARIANA C.; SUMERE, BEATRIZ R.; SILVA, LUIZ G. S.; DA CUNHA, DIOGO T.; BARBERO, GERARDO F.; BEZERRA, ROSANGELA M. N.; ROSTAGNO, MAURICIO A. Simultaneous extraction and separation of bioactive compounds from apple pomace using pressurized liquids coupled on-line with solid-phase extraction. Food Chemistry, v. 318, JUL 15 2020. Web of Science Citations: 0.
LACHOS-PEREZ, DANIEL; BASEGGIO, ANDRESSA M.; TORRES-MAYANGA, PAULO C.; AVILA, PATRICIA F.; TOMPSETT, G. A.; MAROSTICA, MARIO; GOLDBECK, ROSANA; TIMKO, MICHAEL T.; ROSTAGNO, MAURICIO; MARTINEZ, JULIAN; FORSTER-CARNEIRO, TANIA. Sequential subcritical water process applied to orange peel for the recovery flavanones and sugars. JOURNAL OF SUPERCRITICAL FLUIDS, v. 160, JUN 1 2020. Web of Science Citations: 0.

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