Evaluation of ultrasound-assisted pressurized fluid extraction coupled with solid phase extraction for extracting phenolic compounds and terpenes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis)

• Grant number: 20/04067-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: March 01, 2021
• End date: February 28, 2025
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Rodrigo Stein Pizani
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2

Abstract

Rosemary and sage are aromatic herbs used since antiquity for their therapeutic effects on various diseases and metabolic disorders. The potent antioxidant action of these herbs is due to the presence of phenolic compounds and terpenes, and is therefore an attractive for the pharmaceutical and food industries. In order to use these bioactive compounds it is necessary to separate them from the other components of the raw material.In this research project it is proposed to study the possibility of combining the extraction with pressurized liquids (PLE) and ultrasound (UAE) and to couple these techniques with Solid Phase Extraction (SPE) for the purification of phenolic compounds and terpenes from the leaves of rosemary and sage. The first step of the project will be to study the UAPLE extraction process associated with an expansion gas using water as a solvent. The extraction temperature (50-110°C), initial pressure of N2 expansion gas (0-15 bar), ultrasonic power (0-800 W), system pressure (100-300 bar), particle size (0.25 and 1.0 mm), sample amount (0.25-2.0 g), duration (10-30 minutes) and number of extraction cycles (1-5). In the second stage of the project will be carried out the study on the process of extraction and coupled purification (UAPLE-SPE). The proposed process will be carried out in two stages of extraction with different solvents (in the first stage, water: EtOH or methanol 100: 0 at 70:30 v/v in the second stage, water: ethanol or methanol 70:30 to 0: 100 v/v). The interaction of the extraction solvent with the solid phase (Sepra C18, Strata X C18, porapak, zeolite, bentonite and activated carbon) will also be evaluated in the purification step. On the other hand, the duration of each stage (10-30 minutes) required to maximize process efficiency will be evaluated independently as well as the number of extraction cycles (stage I + stage II) required to complete the process (1-5 cycles) using optimized conditions. Finally, the fractionated extracts obtained will evaluate the effects of the compounds present on the cell cycle and the fragmentation of DNA in prostate Cancer cells. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIGANO, JULIANE; STRIEDER, MONIQUE MARTINS; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Application of an integrative system (2D PLExHPLC-PDA) for bioactive compound extraction and online quantification: Advantages, validation, and considerations. Analytica Chimica Acta, v. 1272, p. 9-pg., 2023-06-15. (18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 21/12264-9, 20/03623-2, 20/04067-6)

DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SOSA, FILIPE H. B.; PIZANI, RODRIGO S.; CHAVES, JAISA; VIGANO, JULIANE; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Combining eutectic solvents and pressurized liquid extraction coupled in-line with solid-phase extraction to recover, purify and stabilize anthocyanins from Brazilian berry waste. GREEN CHEMISTRY, v. 25, n. 5, p. 14-pg., 2023-02-21. (21/11022-1, 18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)

DA SILVA, LAISE C.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; BAIAO DIAS, ARTHUR L.; DE SOUZA, MARIANA C.; SANCHES, VITOR L.; CHAVES, JAISA O.; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; ROSTAGNO, MAURICIO A.. Recent advances and trends in extraction techniques to recover polyphenols compounds from apple by-products. FOOD CHEMISTRY-X, v. 12, DEC 30 2021. (20/08421-9, 20/03623-2, 19/24537-0, 20/15774-5, 19/18772-6, 19/13496-0, 18/17089-8, 20/04067-6, 18/14582-5)

DA SILVA, LAISE C.; VIGANO, JULIANE; SANCHES, VITOR L.; DE SOUZA MESQUITA, LEONARDO M.; PIZANI, RODRIGO; ROSTAGNO, MAURICIO A.. Simultaneous extraction and analysis of apple pomace by gradient pressurized liquid extraction coupled in-line with solid-phase extraction and on-line with HPLC. Food Chemistry, v. 407, p. 11-pg., 2022-12-10. (18/14582-5, 19/13496-0, 19/24537-0, 20/04067-6, 20/15774-5)

STRIEDER, MONIQUE MARTINS; BRAGAGNOLO, FELIPE SANCHEZ; PIZANI, RODRIGO S.; ROSTAGNO, MAURICIO ARIEL. The impact of using an expansion gas with high-intensity ultrasound on the pressurized liquid extraction of bioactive compounds from an industrial coffee solid residue. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 92, p. 12-pg., 2024-01-26. (20/04067-6, 22/10469-5, 19/13496-0, 21/12264-9, 18/14582-5)

CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; SANCHES, VITOR L.; CHAVES, JAISA; PIZANI, RODRIGO S.; DA SILVA, LAISE C.; VIGANO, JULIANE; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Recent progress on the recovery of bioactive compounds obtained from propolis as a natural resource: Processes, and applications. Separation and Purification Technology, v. 298, p. 18-pg., 2022-07-06. (18/17089-8, 19/18772-6, 18/14582-5, 20/08421-9, 19/24537-0, 20/15774-5, 20/03623-2, 20/04067-6)

PIZANI, RODRIGO S.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SANCHES, VITOR L.; CHAVES, JAISA O.; SOUZA, MARIANA C.; DA SILVA, LAISE C.; ROSTAGNO, MAURICIO A.. Beyond aroma: A review on advanced extraction processes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis) to produce phenolic acids and diterpenes. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 127, p. 18-pg., 2022-08-02. (18/17089-8, 18/14582-5, 20/08421-9, 19/24537-0, 20/15774-5, 20/03623-2, 19/13496-0, 20/04067-6)

PIZANI, RODRIGO S.; VIGANO, JULIANE; CONTIERI, LETICIA S.; STRIEDER, MONIQUE M.; KAMIKAWASHI, RENAN K.; VILEGAS, WAGNER; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. New selective and sustainable ultrasound-assisted extraction procedure to recover carnosic and rosmarinic acids from Rosmarinus officinalis by sequential use of bio-based solvents. Food Chemistry, v. 435, p. 11-pg., 2023-09-30. (20/08421-9, 20/04067-6, 20/03623-2, 19/13496-0, 21/12264-9, 18/14582-5)

CONTIERI, LETICIAS.; RIBEIRO, TANIA B.; SOSA, FILIPE H. B.; VAZ, BARBARA M. C.; PIZANI, RODRIGO S.; PINTADO, MANUELA; VENTURA, SONIA P. M.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Unlocking the Full Potential of Green Propolis: A Novel Extraction Approach Using Eutectic Solvents for Improved Phenolic Compound Recovery. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 36, p. 13-pg., 2023-08-25. (21/11022-1, 18/14582-5, 20/08421-9, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)