Evaluation of ultrasound-assisted pressurized fluid extraction coupled with solid phase extraction for extracting phenolic compounds and terpenes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis)

• Grant number: 20/04067-6
• Support type: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): March 01, 2021
• Effective date (End): April 30, 2024
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal researcher: Maurício Ariel Rostagno
• Grantee: Rodrigo Stein Pizani
• Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2

Abstract

Rosemary and sage are aromatic herbs used since antiquity for their therapeutic effects on various diseases and metabolic disorders. The potent antioxidant action of these herbs is due to the presence of phenolic compounds and terpenes, and is therefore an attractive for the pharmaceutical and food industries. In order to use these bioactive compounds it is necessary to separate them from the other components of the raw material.In this research project it is proposed to study the possibility of combining the extraction with pressurized liquids (PLE) and ultrasound (UAE) and to couple these techniques with Solid Phase Extraction (SPE) for the purification of phenolic compounds and terpenes from the leaves of rosemary and sage. The first step of the project will be to study the UAPLE extraction process associated with an expansion gas using water as a solvent. The extraction temperature (50-110°C), initial pressure of N2 expansion gas (0-15 bar), ultrasonic power (0-800 W), system pressure (100-300 bar), particle size (0.25 and 1.0 mm), sample amount (0.25-2.0 g), duration (10-30 minutes) and number of extraction cycles (1-5). In the second stage of the project will be carried out the study on the process of extraction and coupled purification (UAPLE-SPE). The proposed process will be carried out in two stages of extraction with different solvents (in the first stage, water: EtOH or methanol 100: 0 at 70:30 v/v in the second stage, water: ethanol or methanol 70:30 to 0: 100 v/v). The interaction of the extraction solvent with the solid phase (Sepra C18, Strata X C18, porapak, zeolite, bentonite and activated carbon) will also be evaluated in the purification step. On the other hand, the duration of each stage (10-30 minutes) required to maximize process efficiency will be evaluated independently as well as the number of extraction cycles (stage I + stage II) required to complete the process (1-5 cycles) using optimized conditions. Finally, the fractionated extracts obtained will evaluate the effects of the compounds present on the cell cycle and the fragmentation of DNA in prostate Cancer cells. (AU)