Multi user equipment approved in grant 2018/14582-5: ultra-high pressure liquid chromatography system (UHPLC), composed by a quaternary pump, autoinjector, oven with selection valve and detector (PDA)

• Grant number: 19/13496-0
• Support Opportunities: Multi-user Equipment Program
• Duration: August 01, 2019 - July 31, 2026
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Maurício Ariel Rostagno
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatrografia líquida
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

This research project proposes the development of a two-dimensional extraction, separation and analysis system (PLEgrad-SPE x UHPLC) by coupling extraction with gradient pressurized liquids (PLEgrad) with solid phase extraction (SPE) in the first dimension and orthogonally with ultra-high pressure chromatography (UHPLC) in the second dimension. The objective is to develop a system that allows the characterization of the chemical composition of natural raw materials in a fast, efficient and with real-time monitoring, while allowing the production of small quantities of extracts obtained through the fractionation of the present compounds. These fractions should be well characterized chemically and with low contamination between them. The system will be validated with several samples, including soybean (Glycine max), Brazilian ginseng (Pfaffia glomerata), black tea and green tea (Camellia sinensis), mate (Ilex paraguariensis), coffee (Coffea arabica), pomegranate granatum), orange peel (Citrus sinensis), sicilian lemon peel (Citrus x limon) apple peel (Malus domestica), jabuticaba bark (Myrciaria cauliflora), rosemary (Rosmarinus officinalis) and sage (Salvia officinalis). Initially the rapid UHPLC analysis methods will be developed that will be used in the second separation dimension. Several analysis conditions (temperature, column size, flow, volume and injection solvent and mobile phase composition) will be evaluated for an ultra-fast separation method (time between samples <2.0 min). Subsequently, different extraction conditions will be evaluated by SPE coupled gradient PLE, including temperature, pressure, sample amount, adsorbent type, flow, solvent and duration of the extraction / elution gradient. This proposal consists of a continuation of the Young Researcher project (FAPESP 2013 / 04304-4), seeking to consolidate this line of research at FCA / UNICAMP and to continue the training of qualified human resources. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANCHES, VITOR L.; CUNHA, TANIZE A.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; FACCIOLI, LUCIA HELENA; BREITKREITZ, MARCIA CRISTINA; ROSTAGNO, MAURICIO A.. Comprehensive analysis of phenolics compounds in citrus fruits peels by UPLC-PDA and UPLC-Q/TOF MS using a fused-core column. FOOD CHEMISTRY-X, v. 14, p. 9-pg., 2022-02-22. (20/08421-9, 19/13496-0, 18/14582-5, 20/15774-5)

CONTIERI, LETICIA S.; DE SOUZA MESQUITA, LEONARDO M.; SANCHES, VITOR L.; VIGANO, JULIANE; MARTINEZ, JULIAN; DA CUNHA, DIOGO T.; ROSTAGNO, MAURICIO A.. Standardization proposal to quality control of propolis extracts commercialized in Brazil : A fingerprinting methodology using a UHPLC-PDA-MS/MS approach. Food Research International, v. 161, p. 11-pg., 2022-08-30. (18/14582-5, 19/13496-0, 20/08421-9, 20/03623-2, 20/15774-5)

STRIEDER, MONIQUE MARTINS; PINAS, JEAN AGUSTIN VELASQUEZ; AMPESE, LARISSA CASTRO; COSTA, JOSIEL MARTINS; CARNEIRO, TANIA FORSTER; ROSTAGNO, MAURICIO ARIEL. Coffee biorefinery: The main trends associated with recovering valuable compounds from solid coffee residues. JOURNAL OF CLEANER PRODUCTION, v. 415, p. 16-pg., 2023-06-17. (21/08696-0, 21/03950-6, 18/14582-5, 19/13496-0, 18/14938-4, 21/12264-9)

DA SILVA, LAISE C.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; BAIAO DIAS, ARTHUR L.; DE SOUZA, MARIANA C.; SANCHES, VITOR L.; CHAVES, JAISA O.; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; ROSTAGNO, MAURICIO A.. Recent advances and trends in extraction techniques to recover polyphenols compounds from apple by-products. FOOD CHEMISTRY-X, v. 12, DEC 30 2021. (20/08421-9, 20/03623-2, 19/24537-0, 20/15774-5, 19/18772-6, 19/13496-0, 18/17089-8, 20/04067-6, 18/14582-5)

RIBEIRO, MAURICIO M. DE SOUZA; VIGANO, JULIANE; DE NOVAIS, NATHALIA STAHL; MESQUITA, LEONARDO M. DE SOUZA; KAMIKAWACHI, RENAN CANUTE; VILEGAS, WAGNER; LOPES, PATRICIA SANTOS; DA SILVA, CAMILA SOARES; ROSTAGNO, MAURICIO ARIEL; VEGGI, PRISCILA CARVALHO. The effect of ultrasound on improving the extraction of tannins from the Stryphnodendron adstringens bark. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 33, p. 14-pg., 2023-03-10. (20/15774-5, 18/14582-5, 19/13496-0, 20/07575-2)

CONTIERI, LETICIAS.; RIBEIRO, TANIA B.; SOSA, FILIPE H. B.; VAZ, BARBARA M. C.; PIZANI, RODRIGO S.; PINTADO, MANUELA; VENTURA, SONIA P. M.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Unlocking the Full Potential of Green Propolis: A Novel Extraction Approach Using Eutectic Solvents for Improved Phenolic Compound Recovery. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 36, p. 13-pg., 2023-08-25. (21/11022-1, 18/14582-5, 20/08421-9, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)

DA SILVA, LAISE C.; VIGANO, JULIANE; SANCHES, VITOR L.; DE SOUZA MESQUITA, LEONARDO M.; PIZANI, RODRIGO; ROSTAGNO, MAURICIO A.. Simultaneous extraction and analysis of apple pomace by gradient pressurized liquid extraction coupled in-line with solid-phase extraction and on-line with HPLC. Food Chemistry, v. 407, p. 11-pg., 2022-12-10. (18/14582-5, 19/13496-0, 19/24537-0, 20/04067-6, 20/15774-5)

PIZANI, RODRIGO S.; VIGANO, JULIANE; DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SANCHES, VITOR L.; CHAVES, JAISA O.; SOUZA, MARIANA C.; DA SILVA, LAISE C.; ROSTAGNO, MAURICIO A.. Beyond aroma: A review on advanced extraction processes from rosemary (Rosmarinus officinalis) and sage (Salvia officinalis) to produce phenolic acids and diterpenes. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 127, p. 18-pg., 2022-08-02. (18/17089-8, 18/14582-5, 20/08421-9, 19/24537-0, 20/15774-5, 20/03623-2, 19/13496-0, 20/04067-6)

MESQUITA, LEONARDO M. DE SOUZA; CONTIERI, LETICIA S.; SANCHES, VITOR L.; KAMIKAWACHI, RENAN; SOSA, FILIPE H. B.; VILEGAS, WAGNER; ROSTAGNO, MAURICIO A.. Fast and green universal method to analyze and quantify anthocyanins in natural products by UPLC-PDA. Food Chemistry, v. 428, p. 11-pg., 2023-12-01. (21/11022-1, 18/14582-5, 20/08421-9, 19/13496-0, 20/03623-2, 21/11023-8)

SGANZERLA, WILLIAM GUSTAVO; VIGANO, JULIANE; CASTRO, LUIZ EDUARDO NOCHI; MACIEL-SILVA, FRANCISCO WESHLEY; ROSTAGNO, MAURICIO A.; MUSSATTO, SOLANGE I.; FORSTER-CARNEIRO, TANIA. Recovery of sugars and amino acids from brewers' spent grains using subcritical water hydrolysis in a single and two sequential semi-continuous flow-through reactors. Food Research International, v. 157, p. 11-pg., 2022-06-10. (19/26925-7, 20/15774-5, 18/05999-0, 19/13496-0)

CHAVES, JAISA O.; SANCHES, VITOR L.; VIGANO, JULIANE; MESQUITA, LEONARDO M. DE SOUZA; DE SOUZA, MARIANA C.; DA SILVA, LAISE C.; ACUNHA, TANIZE; FACCIOLI, LUCIA H.; ROSTAGNO, MAURICIO A.. Integration of pressurized liquid extraction and in-line solid-phase extraction to simultaneously extract and concentrate phenolic compounds from lemon peel (Citrus limon L.). Food Research International, v. 157, p. 12-pg., 2022-07-01. (13/04304-4, 20/08421-9, 15/00658-1, 19/13496-0, 18/14582-5, 20/15774-5)

VIGANO, JULIANE; DE AGUIAR, ANA CAROLINA; VEGGI, PRISCILLA CARVALHO; SANCHES, VITOR L.; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Techno-economic evaluation for recovering phenolic compounds from acai (Euterpe oleracea) by-product by pressurized liquid extraction. JOURNAL OF SUPERCRITICAL FLUIDS, v. 179, JAN 2022. (20/07575-2, 17/23670-2, 16/18910-1, 19/13496-0, 20/15774-5)

CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; SANCHES, VITOR L.; VIGANO, JULIANE; KAMIKAWACHI, RENAN CANUTE; VILEGAS, WAGNER; ROSTAGNO, MAURICIO A.. Ultra-high-performance liquid chromatography using a fused-core particle column for fast analysis of propolis phenolic compounds. JOURNAL OF SEPARATION SCIENCE, v. 46, n. 3, p. 12-pg., 2022-12-09. (18/14582-5, 19/13496-0, 20/08421-9, 20/15774-5)

VIGANO, JULIANE; STRIEDER, MONIQUE MARTINS; PIZANI, RODRIGO S.; CONTIERI, LETICIA S.; MESQUITA, LEONARDO M. DE SOUZA; ROSTAGNO, MAURICIO A.. Application of an integrative system (2D PLExHPLC-PDA) for bioactive compound extraction and online quantification: Advantages, validation, and considerations. Analytica Chimica Acta, v. 1272, p. 9-pg., 2023-06-15. (18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 21/12264-9, 20/03623-2, 20/04067-6)

DE SOUZA MESQUITA, LEONARDO M.; CONTIERI, LETICIA S.; SOSA, FILIPE H. B.; PIZANI, RODRIGO S.; CHAVES, JAISA; VIGANO, JULIANE; VENTURA, SONIA P. M.; ROSTAGNO, MAURICIO A.. Combining eutectic solvents and pressurized liquid extraction coupled in-line with solid-phase extraction to recover, purify and stabilize anthocyanins from Brazilian berry waste. GREEN CHEMISTRY, v. 25, n. 5, p. 14-pg., 2023-02-21. (21/11022-1, 18/14582-5, 20/08421-9, 20/15774-5, 19/13496-0, 20/03623-2, 21/11023-8, 20/04067-6)

OLIVEIRA, AMANDA MELLISSA BEZERRA; VIGANO, JULIANE; SANCHES, VITOR L.; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Extraction of potential bioactive compounds from industrial Tahiti lime (Citrus latifolia Tan.) by-product using pressurized liquids and ultrasound-assisted extraction. Food Research International, v. 157, p. 10-pg., 2022-05-24. (19/13496-0, 20/15774-5, 21/01008-1)

SANCHES, VITOR L.; DE SOUZA MESQUITA, LEONARDO M.; VIGANO, JULIANE; CONTIERI, LETICIA S.; PIZANI, RODRIGO; CHAVES, JAISA; DA SILVA, LAISE CAPELASSO; DE SOUZA, MARIANA CORREA; BREITKREITZ, MARCIA CRISTINA; ROSTAGNO, MAURICIO A.. Insights on the Extraction and Analysis of Phenolic Compounds from Citrus Fruits: Green Perspectives and Current Status. CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY, v. N/A, p. 27-pg., 2022-08-16. (18/14582-5, 20/15774-5, 19/13496-0, 19/24537-0, 20/08421-9, 20/03623-2)