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Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology

Grant number: 13/04304-4
Support type:Research Grants - Young Investigators Grants
Duration: July 01, 2013 - June 30, 2018
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Maurício Ariel Rostagno
Grantee:Maurício Ariel Rostagno
Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
Assoc. researchers:Diego Tresinari dos Santos ; Julian Martínez ; Juliana Martin Do Prado ; Maria Angela de Almeida Meireles Petenate ; Tânia Forster Carneiro
Associated grant(s):18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
Associated scholarship(s):17/24142-0 - Production of breads enriched with bioactive compounds of mate (Ilex paraguariensis), BP.IC
17/17708-7 - Development of functional bread with bioactive compounds of apple peels (Malus domestica Borkh), BP.IC
17/11159-1 - Development of functional cookies with residues from pomegranate (Punica granatum L.), BP.IC
+ associated scholarships 16/20016-7 - Extraction of bioactive compounds from husks of pomegranate (Punica granatum L.) using pressurized liquids assisted by ultrasound, BP.MS
16/19930-6 - Evaluation of the extraction with pressurized liquid assisted by ultrasound coupled to the solid phase extraction for the purification of phenolic compounds from tea (Camellia sinensis), BP.MS
13/15049-5 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology, BP.JP - associated scholarships

Abstract

This research project proposes the development of an integrated analysis system using supercritical technology and of the necessary methodology for the determination of several bioactive compounds in natural products. The proposed integrated system consists of the coupling between the sample preparation (extraction and purification) and chromatographic analysis steps. Sample preparation is accomplished through a combination of supercritical fluid extraction (SFE) or pressurized liquid extraction (PLE) with ultrasound, and the on-line coupling with the purification by solid phase extraction (SPE), which in turn is also coupled on-line with the analysis by supercritical fluid chromatography (SFC). The design of the integrated system allows performing different procedures for the extraction, purification and analysis individually (UAPLE; UASFE; SPE; SFC) or as a single online process (UAPLE/UASFE-SPE, SPE-SFC; UAPLE / UASFE-SPE-SFC). This system will be used for the development of methodologies necessary for the determination of a wide variety of bioactive compounds (phenolic acids, catechins, flavonoids, isoflavones, alkaloids, turmerones, curcuminoids, carotenoids and ecdysteroids) present in different types of samples (black tea, mate, coffee, soybeans, mushrooms, annatto seeds, turmeric rhizomes, Brazilian ginseng rhizomes and commercial extracts of these natural products). Finally an economic evaluation of the methods developed will be carried out through simulations in the software SuperPro ® Designer. (AU)

Scientific publications (26)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHAVES, FERNANDA MACHADO; BETIM PAVAN, ISADORA CAROLINA; SALVINO DA SILVA, LUIZ GUILHERME; DE FREITAS, LIDIA BROGLIO; ROSTAGNO, MAURICIO ARIEL; COSTA ANTUNES, ADRIANE ELISABETE; NEVES BEZERRA, ROSANGELA MARIA; SIMABUCO, FERNANDO MOREIRA. Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway. PLANT FOODS FOR HUMAN NUTRITION, v. 75, n. 1, p. 54-62, MAR 2020. Web of Science Citations: 0.
SALVINO DA SILVA, LUIZ GUILHERME; MORELLI, ANA PAULA; BETIM PAVAN, ISADORA CAROLINA; TAVARES, MARIANA ROSOLEN; PESTANA, NATHALIE FORTES; ROSTAGNO, MAURICIO ARIEL; SIMABUCO, FERNANDO MOREIRA; NEVES BEZERRA, ROSANGELA MARIA. Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro. Phytotherapy Research, v. 34, n. 6 JAN 2020. Web of Science Citations: 0.
TORRES-MAYANGA, PAULO C.; LACHOS-PEREZ, DANIEL; MUDHOO, ACKMEZ; KUMAR, SUNIL; BROWN, AVERY B.; TYUFEKCHIEV, MAKSIM; DRAGONE, GIULIANO; MUSSATTO, I, SOLANGE; ROSTAGNO, MAURICIO A.; TIMKO, MICHAEL; FORSTER-CARNEIRO, T. Production of biofuel precursors and value-added chemicals from hydrolysates resulting from hydrothermal processing biomass: A review. BIOMASS & BIOENERGY, v. 130, NOV 2019. Web of Science Citations: 3.
DA FONSECA MACHADO, ANA PAULA; SUMERE, BEATRIZ ROCCHETTI; MEKARU, CAROLINA; MARTINEZ, JULIAN; NEVES BEZERRA, ROSANGELA MARIA; ROSTAGNO, MAURICIO ARIEL. Extraction of polyphenols and antioxidants from pomegranate peel using ultrasound: influence of temperature, frequency and operation mode. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 54, n. 9, p. 2792-2801, SEP 2019. Web of Science Citations: 0.
SALCES, FRANZ R.; ROSTAGNO, MAURICIO A.; AMAYA-FARFAN, JAIME. Novel process of hydration, followed by incubation and thermal processing, for high isoflavone bioconversion in soybeans. Food Research International, v. 121, p. 691-696, JUL 2019. Web of Science Citations: 0.
TORRES-MAYANGA, P. C.; AZAMBUJA, S. P. H.; TYUFEKCHIEV, M.; TOMPSETT, G. A.; TIMKO, M. T.; GOLDBECK, R.; ROSTAGNO, M. A.; FORSTER-CARNEIRO, T. Subcritical water hydrolysis of brewer's spent grains: Selective production of hemicellulosic sugars (C-5 sugars). JOURNAL OF SUPERCRITICAL FLUIDS, v. 145, p. 19-30, MAR 2019. Web of Science Citations: 2.
LACHOS-PEREZ, D.; BASEGGIO, ANDRESSA MARA; MAYANGA-TORRES, P. C.; MAROSTICA JUNIOR, MARIO ROBERTO; ROSTAGNO, M. A.; MARTINEZ, JULIAN; FORSTER-CARNEIRO, T. Subcritical water extraction of flavanones from defatted orange peel. JOURNAL OF SUPERCRITICAL FLUIDS, v. 138, p. 7-16, AUG 2018. Web of Science Citations: 18.
LORIZOLA, ISABELA M.; FURLAN, CIBELE P. B.; PORTOVEDO, MARIANA; MILANSKI, MARCIANE; BOTELHO, PATRICIA B.; BEZERRA, ROSANGELA M. N.; SUMERE, BEATRIZ R.; ROSTAGNO, MAURICIO A.; CAPITANI, CAROLINE D. Beet Stalks and Leaves (Beta vulgaris L.) Protect Against High-Fat Diet-Induced Oxidative Damage in the Liver in Mice. NUTRIENTS, v. 10, n. 7 JUL 2018. Web of Science Citations: 6.
CARVALHO, PEDRO I. N.; OSORIO-TOBON, JUAN F.; ZABOT, GIOVANI L.; MEIRELES, M. ANGELA A. Spatial and temporal temperature distributions in fixed beds undergoing supercritical fluid extraction. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 47, p. 504-516, JUN 2018. Web of Science Citations: 0.
MERLINI, VANESSA VANDERLEIA; PENA, FABIOLA DE LIMA; DA CUNHA, DIOGO THIMOTEO; DE OLIVEIRA, JULICRISTIE MACHADO; ROSTAGNO, MAURICIO ARIEL; COSTA ANTUNES, ADRIANE ELISABETE. Microbiological Quality of Organic and Conventional Leafy Vegetables. JOURNAL OF FOOD QUALITY, 2018. Web of Science Citations: 1.
LACHOS-PEREZ, D.; TOMPSETT, G. A.; GUERRA, P.; TIMKO, M. T.; ROSTAGNO, M. A.; MARTINEZ, JULIAN; FORSTER-CARNEIRO, T. Sugars and char formation on subcritical water hydrolysis of sugarcane straw. Bioresource Technology, v. 243, p. 1069-1077, NOV 2017. Web of Science Citations: 13.
FORSTER-CARNEIRO, T.; BERNI, M. D.; LACHOS-PEREZ, D.; DORILEO, I. L.; ROSTAGNO, M. A. Characterization and analysis of specific energy consumption in the Brazilian agricultural sector. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, v. 14, n. 10, p. 2077-2092, OCT 2017. Web of Science Citations: 2.
MARTINEZ-JIMENEZ, FERNAN D.; MACIE PINTO, MARIA PAULA; MUDHOO, ACKMEZ; NEVES, THIAGO DE ALENCAR; ROSTAGNO, MAURICIO A.; FORSTER-CARNEIRO, TANIA. Influence of ultrasound irradiation pre-treatment in biohythane generation from the thermophilic anaerobic co-digestion of sugar production residues. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 5, n. 4, p. 3749-3758, AUG 2017. Web of Science Citations: 6.
VARDANEGA, RENATA; CARVALHO, PEDRO I. N.; SANTOS, DIEGO T.; MEIRELES, M. ANGELA A. Obtaining prebiotic carbohydrates and beta-ecdysone from Brazilian ginseng by subcritical water extraction. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 42, p. 73-82, AUG 2017. Web of Science Citations: 10.
VARDANEGA, RENATA; SANTOS, DIEGO T.; MEIRELES, M. ANGELA A. Proposal for fractionating Brazilian ginseng extracts: Process intensification approach. Journal of Food Engineering, v. 196, p. 73-80, MAR 2017. Web of Science Citations: 3.
GARCIA-MENDOZA, MARIA DEL PILAR; ESPINOSA-PARDO, FABER ARIEL; BASEGGIO, ANDRESSA MARA; FERNANDEZ BARBERO, GERARDO; MAROSTICA JUNIOR, MARIO ROBERTO; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Extraction of phenolic compounds and anthocyanins from jucara (Euterpe edulis Mart.) residues using pressurized liquids and supercritical fluids. JOURNAL OF SUPERCRITICAL FLUIDS, v. 119, p. 9-16, JAN 2017. Web of Science Citations: 44.
LACHOS-PEREZ, D.; BROWN, A. B.; MUDHOO, A.; MARTINEZ, J.; TIMKO, M. T.; ROSTAGNO, M. A.; FORSTER-CAMEIRO, T. Applications of subcritical and supercritical water conditions for extraction, hydrolysis, gasification, and carbonization of biomass: a critical review. BIOFUEL RESEARCH JOURNAL-BRJ, v. 4, n. 2, p. 611-626, SPR 2017. Web of Science Citations: 13.
FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; FERNANDEZ BARBERO, GERARDO; NOGUEIRA, GISLAINE CHRYSTINA; ROSTAGNO, MAURICIO ARIEL; DE ALMEIDA MEIRELES, MARIA ANGELA. Fast analysis of curcuminoids from turmeric (Curcuma longa L.) by high-performance liquid chromatography using a fused-core column. Food Chemistry, v. 200, p. 167-174, JUN 1 2016. Web of Science Citations: 20.
FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A. Process integration for turmeric products extraction using supercritical fluids and pressurized liquids: Economic evaluation. FOOD AND BIOPRODUCTS PROCESSING, v. 98, p. 227-235, APR 2016. Web of Science Citations: 21.
PRADO, JULIANA M.; LACHOS-PEREZ, DANIEL; FORSTER-CARNEIRO, TANIA; ROSTAGNO, MAURICIO A. Sub- and supercritical water hydrolysis of agricultural and food industry residues for the production of fermentable sugars: A review. FOOD AND BIOPRODUCTS PROCESSING, v. 98, p. 95-123, APR 2016. Web of Science Citations: 35.
FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A. Precipitation of curcuminoids from an ethanolic turmeric extract using a supercritical antisolvent process. JOURNAL OF SUPERCRITICAL FLUIDS, v. 108, p. 26-34, FEB 2016. Web of Science Citations: 11.
CARVALHO, PEDRO I. N.; FELIPE OSORIO-TOBON, J.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A. Techno-economic evaluation of the extraction of turmeric (Curcuma longa L.) oil and ar-turmerone using supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, v. 105, n. SI, p. 44-54, OCT 2015. Web of Science Citations: 29.
OSORIO-TOBON, J. FELIPE; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A. Extraction of curcuminoids from deflavored turmeric (Curcuma longa L.) using pressurized liquids: Process integration and economic evaluation. JOURNAL OF SUPERCRITICAL FLUIDS, v. 95, p. 167-174, NOV 2014. Web of Science Citations: 43.
BORGES, ENDLER M.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A. Sub-2 mu m fully porous and partially porous (core-shell) stationary phases for reversed phase liquid chromatography. RSC ADVANCES, v. 4, n. 44, p. 22875-22887, 2014. Web of Science Citations: 17.
ROSTAGNO, MAURICIO A.; DEBIEN, ISABEL C. N.; VARDANEGA, RENATA; NOGUEIRA, GISLAINE C.; BARBERO, GERARDO F.; MEIRELES, M. ANGELA A. Fast analysis of beta-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column. ANALYTICAL METHODS, v. 6, n. 8, p. 2452-2459, 2014. Web of Science Citations: 10.
ZABOT, GIOVANI L.; MORAES, MOYSES N.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A. Fast analysis of phenolic terpenes by high-performance liquid chromatography using a fused-core column. ANALYTICAL METHODS, v. 6, n. 18, p. 7457-7468, 2014. Web of Science Citations: 7.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

SISTEMA INTEGRADO DE ANÁLISES PARA A DETERMINAÇÃO DE COMPOSTOS QUÍMICOS E USO BR1020150309392 - Universidade Estadual de Campinas (UNICAMP) . Mauricio Ariel Rostagno - December 2015, 10