Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology

• Grant number: 13/04304-4
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: July 01, 2013
• End date: June 30, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Maurício Ariel Rostagno
• Grantee: Maurício Ariel Rostagno
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• City of the host institution: Limeira
• Associated researchers: Diego Tresinari dos Santos ; Julian Martínez ; Juliana Martin Do Prado ; Maria Angela de Almeida Meireles Petenate ; Tânia Forster Carneiro
• Associated research grant(s): 18/14582-5 - Development of a two-dimensional extraction, separation and analysis system for evaluating the bioactivity of phytochemicals, AP.JP2
• Associated scholarship(s): 17/24142-0 - Production of breads enriched with bioactive compounds of mate (Ilex paraguariensis), BP.IC; 17/17708-7 - Development of functional bread with bioactive compounds of apple peels (Malus domestica Borkh), BP.IC; 17/11159-1 - Development of funcional cookies with residues from pomegranate (Punica granatum L.), BP.IC; + associated scholarships 16/20016-7 - Extraction of bioactive compounds from husks of pomegranate (Punica granatum L.) using pressurized liquids assisted by ultrasound, BP.MS; 16/19930-6 - Evaluation of the extraction with pressurized liquid assisted by ultrasound coupled to the solid phase extraction for the purification of phenolic compounds from tea (Camellia sinensis), BP.MS; 13/15049-5 - Development of integrated systems for the analysis of bioactive compounds in natural products employing supercritical technology, BP.JP - associated scholarships

Abstract

This research project proposes the development of an integrated analysis system using supercritical technology and of the necessary methodology for the determination of several bioactive compounds in natural products. The proposed integrated system consists of the coupling between the sample preparation (extraction and purification) and chromatographic analysis steps. Sample preparation is accomplished through a combination of supercritical fluid extraction (SFE) or pressurized liquid extraction (PLE) with ultrasound, and the on-line coupling with the purification by solid phase extraction (SPE), which in turn is also coupled on-line with the analysis by supercritical fluid chromatography (SFC). The design of the integrated system allows performing different procedures for the extraction, purification and analysis individually (UAPLE; UASFE; SPE; SFC) or as a single online process (UAPLE/UASFE-SPE, SPE-SFC; UAPLE / UASFE-SPE-SFC). This system will be used for the development of methodologies necessary for the determination of a wide variety of bioactive compounds (phenolic acids, catechins, flavonoids, isoflavones, alkaloids, turmerones, curcuminoids, carotenoids and ecdysteroids) present in different types of samples (black tea, mate, coffee, soybeans, mushrooms, annatto seeds, turmeric rhizomes, Brazilian ginseng rhizomes and commercial extracts of these natural products). Finally an economic evaluation of the methods developed will be carried out through simulations in the software SuperPro ® Designer. (AU)

Scientific publications

(39)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

CARVALHO, PEDRO I. N.; FELIPE OSORIO-TOBON, J.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A.. Techno-economic evaluation of the extraction of turmeric (Curcuma longa L.) oil and ar-turmerone using supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, v. 105, n. SI, p. 44-54, OCT 2015. (13/04304-4, 12/10685-8, 13/20758-5)

CHAVES, FERNANDA MACHADO; BETIM PAVAN, ISADORA CAROLINA; SALVINO DA SILVA, LUIZ GUILHERME; DE FREITAS, LIDIA BROGLIO; ROSTAGNO, MAURICIO ARIEL; COSTA ANTUNES, ADRIANE ELISABETE; NEVES BEZERRA, ROSANGELA MARIA; SIMABUCO, FERNANDO MOREIRA. Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway. PLANT FOODS FOR HUMAN NUTRITION, v. 75, n. 1, p. 54-62, MAR 2020. (15/07299-7, 18/14818-9, 16/06457-0, 12/13558-7, 13/04304-4)

TORRES-MAYANGA, PAULO C.; LACHOS-PEREZ, DANIEL; MUDHOO, ACKMEZ; KUMAR, SUNIL; BROWN, AVERY B.; TYUFEKCHIEV, MAKSIM; DRAGONE, GIULIANO; MUSSATTO, I, SOLANGE; ROSTAGNO, MAURICIO A.; TIMKO, MICHAEL; et al. Production of biofuel precursors and value-added chemicals from hydrolysates resulting from hydrothermal processing biomass: A review. BIOMASS & BIOENERGY, v. 130, NOV 2019. (18/05999-0, 13/04304-4, 11/19817-1, 18/14938-4)

CARVALHO, PEDRO I. N.; OSORIO-TOBON, JUAN F.; ZABOT, GIOVANI L.; MEIRELES, M. ANGELA A.. Spatial and temporal temperature distributions in fixed beds undergoing supercritical fluid extraction. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 47, p. 504-516, JUN 2018. (12/10685-8, 15/13299-0, 13/04304-4, 13/20758-5)

FORSTER-CARNEIRO, T.; BERNI, M. D.; LACHOS-PEREZ, D.; DORILEO, I. L.; ROSTAGNO, M. A.. Characterization and analysis of specific energy consumption in the Brazilian agricultural sector. INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY, v. 14, n. 10, p. 2077-2092, OCT 2017. (13/04304-4, 11/19817-1)

LACHOS-PEREZ, D.; TOMPSETT, G. A.; GUERRA, P.; TIMKO, M. T.; ROSTAGNO, M. A.; MARTINEZ, JULIAN; FORSTER-CARNEIRO, T.. Sugars and char formation on subcritical water hydrolysis of sugarcane straw. Bioresource Technology, v. 243, p. 1069-1077, NOV 2017. (15/11932-7, 13/04304-4, 11/19817-1)

MARTINEZ-JIMENEZ, FERNAN D.; MACIE PINTO, MARIA PAULA; MUDHOO, ACKMEZ; NEVES, THIAGO DE ALENCAR; ROSTAGNO, MAURICIO A.; FORSTER-CARNEIRO, TANIA. Influence of ultrasound irradiation pre-treatment in biohythane generation from the thermophilic anaerobic co-digestion of sugar production residues. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 5, n. 4, p. 3749-3758, AUG 2017. (13/04304-4, 11/19817-1)

LACHOS-PEREZ, D.; BROWN, A. B.; MUDHOO, A.; MARTINEZ, J.; TIMKO, M. T.; ROSTAGNO, M. A.; FORSTER-CAMEIRO, T.. Applications of subcritical and supercritical water conditions for extraction, hydrolysis, gasification, and carbonization of biomass: a critical review. BIOFUEL RESEARCH JOURNAL-BRJ, v. 4, n. 2, p. 611-626, SPR 2017. (13/04304-4, 11/19817-1)

SALVINO DA SILVA, LUIZ GUILHERME; MORELLI, ANA PAULA; BETIM PAVAN, ISADORA CAROLINA; TAVARES, MARIANA ROSOLEN; PESTANA, NATHALIE FORTES; ROSTAGNO, MAURICIO ARIEL; SIMABUCO, FERNANDO MOREIRA; NEVES BEZERRA, ROSANGELA MARIA. Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro. Phytotherapy Research, v. 34, n. 6, JAN 2020. (16/06457-0, 12/13558-7, 13/04304-4, 18/14818-9)

PRADO, JULIANA M.; LACHOS-PEREZ, DANIEL; FORSTER-CARNEIRO, TANIA; ROSTAGNO, MAURICIO A.. Sub- and supercritical water hydrolysis of agricultural and food industry residues for the production of fermentable sugars: A review. FOOD AND BIOPRODUCTS PROCESSING, v. 98, p. 95-123, APR 2016. (13/04304-4, 11/19817-1)

OSORIO-TOBON, J. FELIPE; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A.. Extraction of curcuminoids from deflavored turmeric (Curcuma longa L.) using pressurized liquids: Process integration and economic evaluation. JOURNAL OF SUPERCRITICAL FLUIDS, v. 95, p. 167-174, NOV 2014. (12/10685-8, 13/04304-4)

BORGES, ENDLER M.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A.. Sub-2 mu m fully porous and partially porous (core-shell) stationary phases for reversed phase liquid chromatography. RSC ADVANCES, v. 4, n. 44, p. 22875-22887, 2014. (13/04304-4, 12/10685-8, 13/15049-5, 11/07466-0)

FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A.. Precipitation of curcuminoids from an ethanolic turmeric extract using a supercritical antisolvent process. JOURNAL OF SUPERCRITICAL FLUIDS, v. 108, p. 26-34, FEB 2016. (12/10685-8, 13/04304-4)

ZABOT, GIOVANI L.; MORAES, MOYSES N.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A.. Fast analysis of phenolic terpenes by high-performance liquid chromatography using a fused-core column. ANALYTICAL METHODS, v. 6, n. 18, p. 7457-7468, 2014. (13/04304-4, 12/10685-8, 13/15049-5)

OLIVEIRA, T. C. G.; INTERLANDI, M. A.; HANLON, K. E.; TORRES-MAYANGA, P. C.; SILVELLO, M. A. C.; TIMKO, M. T.; ROSTAGNO, M. A.; GOLDBECK, R.; FORSTER-CARNEIRO, T.. Integration of Subcritical Water and Enzymatic Hydrolysis to Obtain Fermentable Sugars and Second-Generation Ethanol from Sugarcane Straw. BioEnergy Research, v. 15, n. 2, p. 12-pg., 2021-04-17. (19/08542-3, 18/05999-0, 18/14582-5, 13/04304-4, 18/14938-4)

SILVA, CAMILA TELLES; DA SOUZA, MARIANA CORREA; DA FONSECA MACHADO, ANA PAULA; DO NASCIMENTO, ROBERTO DE PAULA; DA CUNHA, DIOGO THIMOTEO; NEVES BEZERRA, ROSANGELA MARIA; ROSTAGNO, MAURICIO ARIEL. Thermal stability and sensory evaluation of a bioactive extract from roasted coffee (Coffea arabica) beans added at increasing concentrations to conventional bread. JOURNAL OF FOOD PROCESSING AND PRESERVATION, v. 45, n. 11, SEP 2021. (18/14582-5, 16/19930-6, 17/23657-6, 13/04304-4, 18/17089-8)

MERLINI, VANESSA VANDERLEIA; PENA, FABIOLA DE LIMA; DA CUNHA, DIOGO THIMOTEO; DE OLIVEIRA, JULICRISTIE MACHADO; ROSTAGNO, MAURICIO ARIEL; COSTA ANTUNES, ADRIANE ELISABETE. Microbiological Quality of Organic and Conventional Leafy Vegetables. JOURNAL OF FOOD QUALITY, v. N/A, p. 7-pg., 2018-01-01. (13/04304-4)

CHAVES, JAISA O.; MESQUITA, LEONARDO M. DE SOUZA; STRIEDER, MONIQUE M.; CONTIERI, LETICIA S.; PIZANI, RODRIGO S.; SANCHES, VITOR L.; VIGANO, JULIANE; BEZERRA, ROSANGELA M. NEVES; ROSTAGNO, MAURICIO A.. Eco-friendly and high-performance extraction of flavonoids from lemon peel wastes by applying ultrasound-assisted extraction and eutectic solvents. SUSTAINABLE CHEMISTRY AND PHARMACY, v. 39, p. 13-pg., 2024-06-01. (20/15774-5, 15/00658-1, 20/08421-9, 13/04304-4, 19/13496-0, 19/13496-0, 18/14582-5)

MESQUITA, LEONARDO M. DE SOUZA; CONTIERI, LETICIA S.; A. E SILVA, FRANCISCA; BAGINI, RAFAEL HENRIQUE; BRAGAGNOLO, FELIPE S.; STRIEDER, MONIQUE M.; SOSA, FILIPE H. B.; SCHAEFFER, NICOLAS; FREIRE, MARA G.; VENTURA, SONIA P. M.; et al. Path2Green: introducing 12 green extraction principles and a novel metric for assessing sustainability in biomass valorization. GREEN CHEMISTRY, v. 26, n. 19, p. 21-pg., 2024-08-12. (20/15774-5, 21/11022-1, 15/00658-1, 20/08421-9, 13/04304-4, 23/12621-1, 22/10469-5, 20/03623-2, 19/13496-0, 21/11023-8, 21/12264-9, 18/14582-5, 23/05722-6)

SALVINO DA SILVA, LUIZ GUILHERME; MORELLI, ANA PAULA; BETIM PAVAN, ISADORA CAROLINA; TAVARES, MARIANA ROSOLEN; PESTANA, NATHALIE FORTES; ROSTAGNO, MAURICIO ARIEL; SIMABUCO, FERNANDO MOREIRA; NEVES BEZERRA, ROSANGELA MARIA. Protective effects of beet (Beta vulgaris) leaves extract against oxidative stress in endothelial cells in vitro. Phytotherapy Research, v. 34, n. 6, p. 12-pg., 2020-01-27. (16/06457-0, 13/04304-4, 12/13558-7, 18/14818-9)

CHAVES, JAISA O.; SANCHES, VITOR L.; VIGANO, JULIANE; MESQUITA, LEONARDO M. DE SOUZA; DE SOUZA, MARIANA C.; DA SILVA, LAISE C.; ACUNHA, TANIZE; FACCIOLI, LUCIA H.; ROSTAGNO, MAURICIO A.. Integration of pressurized liquid extraction and in-line solid-phase extraction to simultaneously extract and concentrate phenolic compounds from lemon peel (Citrus limon L.). Food Research International, v. 157, p. 12-pg., 2022-07-01. (13/04304-4, 20/08421-9, 15/00658-1, 19/13496-0, 18/14582-5, 20/15774-5)

LORIZOLA, ISABELA MICHELETTI; MIYAMOTO, JOSIANE ERICA; FIGUEIREDO VIEIRA, ANA LUIZA; SUMERE, BEATRIZ ROCCHETTI; NEVES BEZERRA, ROSANGELA MARIA; TORSONI, MARCIO ALBERTO; TORSONI, ADRIANA SOUZA; ROSTAGNO, MAURICIO ARIEL; MILANSKI, MARCIANE; CAPITANI, CAROLINE DARIO. Beet (Beta vulgaris L.) stalk and leaf supplementation changes the glucose homeostasis and inflammatory markers in the liver of mice exposed to a high-fat diet. FOOD CHEMISTRY: MOLECULAR SCIENCES, v. 2, p. 10-pg., 2021-07-30. (13/04304-4, 13/18498-5, 16/24768-3, 15/15035-0)

MERLINI, VANESSA VANDERLEIA; PENA, FABIOLA DE LIMA; DA CUNHA, DIOGO THIMOTEO; DE OLIVEIRA, JULICRISTIE MACHADO; ROSTAGNO, MAURICIO ARIEL; COSTA ANTUNES, ADRIANE ELISABETE. Microbiological Quality of Organic and Conventional Leafy Vegetables. JOURNAL OF FOOD QUALITY, 2018. (13/04304-4)

LORIZOLA, ISABELA M.; FURLAN, CIBELE P. B.; PORTOVEDO, MARIANA; MILANSKI, MARCIANE; BOTELHO, PATRICIA B.; BEZERRA, ROSANGELA M. N.; SUMERE, BEATRIZ R.; ROSTAGNO, MAURICIO A.; CAPITANI, CAROLINE D.. Beet Stalks and Leaves (Beta vulgaris L.) Protect Against High-Fat Diet-Induced Oxidative Damage in the Liver in Mice. NUTRIENTS, v. 10, n. 7, JUL 2018. (13/04304-4, 15/15035-0, 13/18498-5)

TAMBORLIN, LETICIA; SUMERE, BEATRIZ ROCCHETTI; DE SOUZA, MARIANA CORREA; PESTANA, NATHALIE FORTES; AGUIAR, ANA CAROLINA; EBERLIN, MARCOS NOGUEIRA; SIMABUCO, FERNANDO MOREIRA; ROSTAGNO, MAURICIO ARIEL; LUCHESSI, AUGUSTO DUCATI. Characterization of pomegranate peel extracts obtained using different solvents and their effects on cell cycle and apoptosis in leukemia cells. FOOD SCIENCE & NUTRITION, v. 8, n. 10, AUG 2020. (18/17089-8, 15/18119-0, 19/06951-3, 10/18095-0, 18/14818-9, 17/21914-1, 13/04304-4)

DA FONSECA MACHADO, ANA PAULA; SUMERE, BEATRIZ ROCCHETTI; MEKARU, CAROLINA; MARTINEZ, JULIAN; NEVES BEZERRA, ROSANGELA MARIA; ROSTAGNO, MAURICIO ARIEL. Extraction of polyphenols and antioxidants from pomegranate peel using ultrasound: influence of temperature, frequency and operation mode. INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, v. 54, n. 9, p. 2792-2801, SEP 2019. (15/11932-7, 13/04304-4)

VIGANO, JULIANE; SANCHES, VITOR L.; MESQUITA, LEONARDO M. DE SOUZA; DE SOUZA, MARIANA C.; DA SILVA, LAISE C.; CHAVES, JAISA O.; FORSTER-CARNEIRO, TANIA; ROSTAGNO, MAURICIO A.. Comprehensive analysis of phenolic compounds from natural products: Integrating sample preparation and analysis. Analytica Chimica Acta, v. 1178, SEP 15 2021. (19/24537-0, 18/14582-5, 19/18772-6, 20/08421-9, 18/17089-8, 13/04304-4, 18/14938-4, 20/15774-5)

CARVALHO, PEDRO I. N.; FELIPE OSORIO-TOBON, J.; ROSTAGNO, MAURICIO A.; PETENATE, ADEMIR J.; MEIRELES, M. ANGELA A.. Techno-economic evaluation of the extraction of turmeric (Curcuma longa L.) oil and ar-turmerone using supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, v. 105, p. 11-pg., 2015-10-01. (13/20758-5, 13/04304-4, 12/10685-8)

SOUZA, MARIANA C.; SILVA, LAISE C.; CHAVES, JAISA O.; SALVADOR, MAYARA P.; SANCHES, VITOR L.; DA CUNHA, DIOGO T.; CARNEIRO, TANIA FOSTER; ROSTAGNO, MAURICIO A.. Simultaneous extraction and separation of compounds from mate (Ilex paraguariensis) leaves by pressurized liquid extraction coupled with solid-phase extraction and in-line UV detection. FOOD CHEMISTRY: MOLECULAR SCIENCES, v. 2, p. 11-pg., 2021-07-30. (19/24537-0, 18/14582-5, 13/04304-4, 16/19930-6)

LACHOS-PEREZ, D.; BASEGGIO, ANDRESSA MARA; MAYANGA-TORRES, P. C.; MAROSTICA JUNIOR, MARIO ROBERTO; ROSTAGNO, M. A.; MARTINEZ, JULIAN; FORSTER-CARNEIRO, T.. Subcritical water extraction of flavanones from defatted orange peel. JOURNAL OF SUPERCRITICAL FLUIDS, v. 138, p. 7-16, AUG 2018. (13/04304-4, 11/19817-1)

TORRES-MAYANGA, P. C.; AZAMBUJA, S. P. H.; TYUFEKCHIEV, M.; TOMPSETT, G. A.; TIMKO, M. T.; GOLDBECK, R.; ROSTAGNO, M. A.; FORSTER-CARNEIRO, T.. Subcritical water hydrolysis of brewer's spent grains: Selective production of hemicellulosic sugars (C-5 sugars). JOURNAL OF SUPERCRITICAL FLUIDS, v. 145, p. 19-30, MAR 2019. (13/04304-4, 18/05999-0, 16/04602-3, 11/19817-1)

VARDANEGA, RENATA; CARVALHO, PEDRO I. N.; SANTOS, DIEGO T.; MEIRELES, M. ANGELA A.. Obtaining prebiotic carbohydrates and beta-ecdysone from Brazilian ginseng by subcritical water extraction. INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES, v. 42, p. 73-82, AUG 2017. (12/10685-8, 13/04304-4)

VARDANEGA, RENATA; SANTOS, DIEGO T.; MEIRELES, M. ANGELA A.. Proposal for fractionating Brazilian ginseng extracts: Process intensification approach. Journal of Food Engineering, v. 196, p. 73-80, MAR 2017. (12/19304-7, 12/10685-8, 10/16485-5, 13/17260-5, 13/04304-4)

GARCIA-MENDOZA, MARIA DEL PILAR; ESPINOSA-PARDO, FABER ARIEL; BASEGGIO, ANDRESSA MARA; FERNANDEZ BARBERO, GERARDO; MAROSTICA JUNIOR, MARIO ROBERTO; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Extraction of phenolic compounds and anthocyanins from jucara (Euterpe edulis Mart.) residues using pressurized liquids and supercritical fluids. JOURNAL OF SUPERCRITICAL FLUIDS, v. 119, p. 9-16, JAN 2017. (15/11932-7, 13/04304-4, 13/02203-6)

FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; ROSTAGNO, MAURICIO A.; MEIRELES, M. ANGELA A.. Process integration for turmeric products extraction using supercritical fluids and pressurized liquids: Economic evaluation. FOOD AND BIOPRODUCTS PROCESSING, v. 98, p. 227-235, APR 2016. (12/10685-8, 13/04304-4)

ROSTAGNO, MAURICIO A.; DEBIEN, ISABEL C. N.; VARDANEGA, RENATA; NOGUEIRA, GISLAINE C.; BARBERO, GERARDO F.; MEIRELES, M. ANGELA A.. Fast analysis of beta-ecdysone in Brazilian ginseng (Pfaffia glomerata) extracts by high-performance liquid chromatography using a fused-core column. ANALYTICAL METHODS, v. 6, n. 8, p. 2452-2459, 2014. (13/04304-4, 12/10685-8, 13/15049-5)

FELIPE OSORIO-TOBON, J.; CARVALHO, PEDRO I. N.; FERNANDEZ BARBERO, GERARDO; NOGUEIRA, GISLAINE CHRYSTINA; ROSTAGNO, MAURICIO ARIEL; DE ALMEIDA MEIRELES, MARIA ANGELA. Fast analysis of curcuminoids from turmeric (Curcuma longa L.) by high-performance liquid chromatography using a fused-core column. Food Chemistry, v. 200, p. 167-174, JUN 1 2016. (13/04304-4, 12/10685-8, 13/20758-5)

SALCES, FRANZ R.; ROSTAGNO, MAURICIO A.; AMAYA-FARFAN, JAIME. Novel process of hydration, followed by incubation and thermal processing, for high isoflavone bioconversion in soybeans. Food Research International, v. 121, p. 691-696, JUL 2019. (13/04304-4)

MESQUITA, LEONARDO MENDES DE SOUZA; AGUIAR, ETIENE VALERIA; CONTIERI, LETICIA S.; TOGNI, VITORIA B.; FORLI, BEATRIZ CAROLINA A.; SANCHES, VITOR L.; SANTOS, FERNANDA G.; DA SILVA, LUIZ GUILHERME SALVINO; FERNANDES, DERCILIO MARCELO; CAPRILES, VANESSA D.; et al. Repurposing Sunflower Seed Flour for Nutritional and Functional High-Protein Breads within a Circular Economy Framework. ACS FOOD SCIENCE & TECHNOLOGY, v. 5, n. 4, p. 11-pg., 2025-04-09. (18/14582-5, 13/04304-4, 15/00658-1, 20/15774-5, 20/08421-9, 17/10843-6, 19/13496-0)

Filed patent(s) as a result of this research project