Preparation, characterization and application of new stationary phases for high-performance liquid chromatography and electrochromatography

• Grant number: 06/57897-9
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2007
• End date: November 30, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Carol Hollingworth Collins
• Grantee: Carol Hollingworth Collins
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Pesquisadores principais: Isabel Cristina Sales Fontes Jardim ; Pierina Sueli Bonato
• Associated scholarship(s): 12/23518-2 - Preparation of monolithic capillary columns based on poly(methyloctylsiloxane) thermally immobilized onto silica and mixed oxides for use in capillary liquid chromatography, BP.PD; 11/23127-0 - Development and validation of analytical methods for the determination of multiresidues of pesticides in lettuce, using ultra-performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry., BP.DR; 12/01667-6 - Development and validation of analytical methodologies for determination of pesticides in the cultivation of cucumbers, using ultra performance liquid chromatography (UPLC), BP.MS; + associated scholarships 09/10620-0 - Development and Caracterization of polimeric monolithic stationary phases for using in Capillary Electrochromatography, BP.MS; 08/03947-0 - Preparation of packed cartridges with phenil sorbent for solid phase extraction of the polycyclic aromatic hidrocarbons, BP.IC; 07/05674-9 - Evaluation of C18-monoliths for Cappilary Electrochromatography, BP.IC - associated scholarships

Abstract

This project involves the development of a detailed understanding of the preparation and utilization of new stationary phases (SP) for Reversed-Phase High Performance Liquid Chromatography (RP-HPLC), Capillary Electrochromatography (CEC) and Solid Phase Extraction (SFE) and Microextraction (SFME). The new SP will be prepared by sobbing and immobilizing polymers on appropriate supports, such as silica or metalized silica, or by means of chemical reactions for derivatization and/or polymerization. Our knowledge of SP preparation will be extended to the preparation of monolithic phases, a new mode for separations that presents the advantages of rapid analyses and low solvent consumption in both micro-LC and CEC. The project will emphasize studies to develop a better comprehension of the nature of the processes involved in the preparation of SP for RP-HPLC, CEC and the extractions. Physical, chemical and chromatographic methods will be used to characterize the phases. Chromatographic properties will be optimized to give more stable and more efficient chromatographic and electrochromatographic columns. The best phases will then be evaluated for possible applications with diverse environmental and pharmaceutical samples. It is expected that a fundamental understanding of the physical and chemical processes involved in the preparation of these new SP will result in useful products with applications in several different analytical separation modes, including the separations of complex mixtures of real samples. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, CLAUDIO DE CASTRO; GAMA, MARIANA ROBERTO; DA SILVA, GISELE SILVESTRE; ALMEIDA, WANDA PEREIRA; COLLINS, CAROL HOLLINGWORTH; SALES FONTES JARDIM, ISABEL CRISTINA. Synthesis and evaluation of a pentafluorobenzamide stationary phase for HPLC separations in the reversed phase and hydrophilic interaction modes. JOURNAL OF SEPARATION SCIENCE, v. 41, n. 20, p. 3855-3862, OCT 2018. (06/57897-9)

OSHITA, DANIELE; JARDIM, ISABEL C. S. F.. Evaluation of dispersive and cartridge SPE clean-up procedures using the modified QuEChERS method for the analysis of pesticides in strawberries. ANALYTICAL METHODS, v. 7, n. 3, p. 982-989, 2015. (06/57897-9)

GALAVERNA, RENAN; BREITKREITZ, MARCIA C.; PASTRE, JULIO C.. Conversion of D-Fructose to 5-(Hydroxymethyl)furfural: Evaluating Batch and Continuous Flow Conditions by Design of Experiments and In-Line FTIR Monitoring. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 6, n. 3, p. 4220-4230, MAR 2018. (14/25770-6, 16/05636-9, 06/57897-9, 14/26378-2, 13/07607-8)

OSHITA, DANIELE; JARDIM, ISABEL C. S. F.. Comparison of Different Sorbents in the QuEChERS Method for the Determination of Pesticide Residues in Strawberries by LC-MS/MS. Chromatographia, v. 77, n. 19-20, p. 1291-1298, OCT 2014. (06/57897-9)

DANIELE OSHITA; ISABEL C. S. F. JARDIM. COMPARAÇÃO DE MÉTODOS POR CROMATOGRAFIA LÍQUIDA NA DETERMINAÇÃO DE MULTIRRESÍDUOS DE AGROTÓXICOS EM MORANGOS. Química Nova, v. 38, n. 10, p. 1273-1281, 2015-12-00. (06/57897-9)