Optimized Diagnostic Assays Based on Redox Tagged Bioreceptive Interfaces

Autor(es)

Bedatty Fernandes, Flavio C. ^[1] ; Patil, Amol V. ^[2] ; Bueno, Paulo R. ^[1] ; Davis, Jason J. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Nanobion Grp, Dept Phys Chem, Inst Chem, UNESP, Sao Paulo State Univ, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Oxford, Dept Chem, Oxford OX1 3QZ - England

• Tipo de documento: Artigo Científico
• Fonte: Analytical Chemistry; v. 87, n. 24, p. 12137-12144, DEC 15 2015.
• Citações Web of Science: 19

Resumo

Among the numerous label free electronic biomarker assay methodologies now available, impedance based electrochemical capacitance spectroscopy (ECS), based upon mapping the perturbations in interfacial charging of redox elements incorporated into a biologically receptive interface, has recently been shown to be a convenient and highly sensitive mode of transduction and one which, additionally, requires no predoping of analytical solution. We present, herein, a data acquisition and analysis methodology based on frequency resolved immittance function analysis. Ultimately, this enables both a maximization of assay sensitivity and a reduction in assay acquisition time by an order of magnitude. (AU)

• Processo FAPESP: 15/13359-2 - Sistemas de detecção ultrasensíveis livre de rotulação para testes clínicos por meio de imitância eletroquímica
• Beneficiário: Flávio Cesar Bedatty Fernandes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado