Detoxification of Atrazine by Endophytic Streptomyces sp Isolated from Sugarcane and Detection of Nontoxic Metabolite

Autor(es)

Mesquini, Josiane A. ^[1] ; Sawaya, Alexandra C. H. F. ^[2] ; Lopez, Begona G. C. ^[2] ; Oliveira, Valeria M. ^[3] ; Miyasaka, Natalia R. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Res Lab Appl Microbiol, Braganca Paulista, SP - Brazil

^[2] State Univ Campinas UNICAMP, Inst Biol, Dept Plant Biol, Campinas, SP - Brazil

^[3] State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bulletin of Environmental Contamination and Toxicology; v. 95, n. 6, p. 803-809, DEC 2015.
• Citações Web of Science: 7

Resumo

Atrazine is still one of the most used agricultural pesticides worldwide and it has been recognized as a major contaminant of surface and ground water. The aims of this research were to isolate an endophytic microorganism from leaves of sugarcane, evaluate its ability to degrade atrazine, and investigate the formation of metabolites. By sequencing of the 16S rRNA gene, the endophytic isolate atz2 was identified as Streptomyces sp. The reduction in atrazine concentration by Streptomyces sp. atz2 was 98 % and UHPLC-MS/MS analyses showed the appearance of an unknown metabolite observed as m/z 311. Ecotoxicity tests with an aquatic organism, Daphnia similis, confirmed that this metabolite was nontoxic. This mechanism of detoxification of atrazine is different from the ones of other free-living microorganisms that inhabit the soil or rhizosphere. The results show new aspects of atrazine detoxification, highlighting a new role of endophytic bacteria in plants. (AU)

• Processo FAPESP: 11/50917-2 - Prospecção de microorganismos endofíticos para degradar compostos tóxicos utilizados na cultura canavieira
• Beneficiário: Natalia Reiko Sato Miyasaka
• Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Regular
• Processo FAPESP: 08/58035-6 - Control of lignin biosynthesis in sugar cane: many gaps still to be filled
• Beneficiário: Paulo Mazzafera
• Linha de fomento: Auxílio à Pesquisa - Programa BIOEN - Temático