Microcystins and cylindrospermopsins molecular markers for the detection of toxic cyanobacteria: a case study of northeastern Brazilian reservoirs

Autor(es)

Lorenzi, Adriana Sturion ^[1] ; Chia, Mathias Ahii ^[1] ; Piccin-Santos, Viviane ^{[1, 2]} ; Bittencourt-Oliveira, Maria do Carmo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencias Biol, BR-13418970 Piracicaba, SP - Brazil

^[2] Univ Estadual Paulista, Programa Posgrad Ciencias Biol, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LIMNETICA; v. 34, n. 2, p. 269-281, DEC 2015.
• Citações Web of Science: 2

Resumo

The presence of potentially toxic cyanobacteria in 10 northeastern Brazilian reservoirs used for public water supply was evaluated with molecular markers specific to genes of microcystins (MC) and cylindrospermopsins (CYN). The detection of the MC genes mcyA and mcyB was determined using 3 different primer pairs. Genes of CYN were assessed by PCR amplification of PKS and PS genes. Amplicons of the different cyanotoxin genes were subjected to sequencing. Genes coding for MC (mcyA and/or mcyB) were found in all of the analysed samples. Despite the presence of the mcyB gene and the subsequent immunological detection of MC, the absence of mcyA was observed in several samples. In general, the primers targeting the PKS gene were more specific, while those for the PS gene gave uneven PCR amplification. In addition, the detection of CYN was only possible when the samples were positive for both the PKS and the PS genes. These findings show that, when working with environmental samples, the use of molecular markers for the detection of potentially toxic cyanobacteria may generate variable results, and consideration should be given to possible phytogeographic genetic variations. (AU)

• Processo FAPESP: 13/11306-3 - Efeitos de anatoxina-a e cilindrospermopsina em diferentes concentrações de nitrogênio e irradiância na fisiologia de Microcystis aeruginosa (Cyanobacteria)e Scenedesmus acuminatus (Chlorophyta)
• Beneficiário: Mathias Ahii Chia
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/56534-5 - Filogenia molecular de cianobactérias baseada em sequências do 16S-23S ITS e pc-igs: investigação de transferência lateral do PC
• Beneficiário: Viviane Piccin dos Santos
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/01913-2 - Metagenômica comparativa de florações de cianobactérias em reservatórios de água do Estado de Pernambuco.
• Beneficiário: Adriana Sturion Lorenzi
• Modalidade de apoio: Bolsas no Brasil - Jovens Pesquisadores