Antimicrobial Potential of Papain Chemomechanical Agent on Streptococcus Mutans and Lactobacillus Casei Followed by the Use of Self-Etching Adhesive Systems

Autor(es)

Basting, Roberta Tarkany ^[1] ; Goncalves, Fernanda Rezende ^[1] ; Gomes Franca, Fabiana Mantovani ^[1] ; Botelho do Amaral, Flavia Lucisano ^[1] ; Florio, Flavia Martao ^[1]

Afiliação do(s) autor(es)

^[1] Sao Leopoldo Mand Res Inst, Sch Dent, Dept Restorat Dent, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Clinical Pediatric Dentistry; v. 40, n. 1, p. 62-68, WIN 2016.
• Citações Web of Science: 1

Resumo

Objective: This study evaluated the antimicrobial effect of papain-based gel (Papacarie/ P) followed by self-etching adhesive systems with MDPB monomer (Clearfil Protect Bond/ PB) and without (Clearfil SE Bond/ Cl) on Streptococcus mutans (Sm) and Lactobacillus casei (Lc). Study design: The dentin of twenty human teeth was exposed to prepare four cylindrical cavities in each tooth. The cavities were incubated with Sm or Lc. One cavity from each tooth served as contamination control (positive control group); the other three were treated with P, P+CL and P+PB. The cavities were sealed and after 72 h, dentin samples were collected and microbial cultivation was performed. Microbial count was undertaken (CFUs/mg) according to the morphological characteristics for Sm and Lc. Analysis of variance and Tukey's test were applied (alpha=5%). Results: For Sm, groups P+Cl and P+PB had lower microbial count than group P (no statistical differences between P+Cl and P+PB). For Lc, group P+Cl had microbial count similar to group P There was statistical difference between cavities treated with P and P+PB but none between groups P+Cl and P+PB. After using P, both self-etching adhesives showed antimicrobial potential, although Clearfil Protect Bond proved better against Lc. Conclusions: Both self-etching adhesives used after application of Papacarie showed antimicrobial potential, although Clearfil Protect Bond proved more effective against Lactobacillus casei. (AU)

• Processo FAPESP: 09/14737-0 - Potencial antimicrobiano de um agente químico contendo papaína sobre microrganismos cariogênicos seguida pela aplicação de sistemas adesivos autocondicionantes e de sistemas adesivos autocondicionantes submetidos a diferentes tempos e fontes de ativação
• Beneficiário: Roberta Tarkany Basting Höfling
• Modalidade de apoio: Auxílio à Pesquisa - Regular