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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antimicrobial Potential of Papain Chemomechanical Agent on Streptococcus Mutans and Lactobacillus Casei Followed by the Use of Self-Etching Adhesive Systems

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Author(s):
Basting, Roberta Tarkany [1] ; Goncalves, Fernanda Rezende [1] ; Gomes Franca, Fabiana Mantovani [1] ; Botelho do Amaral, Flavia Lucisano [1] ; Florio, Flavia Martao [1]
Total Authors: 5
Affiliation:
[1] Sao Leopoldo Mand Res Inst, Sch Dent, Dept Restorat Dent, Campinas, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Journal of Clinical Pediatric Dentistry; v. 40, n. 1, p. 62-68, WIN 2016.
Web of Science Citations: 1
Abstract

Objective: This study evaluated the antimicrobial effect of papain-based gel (Papacarie/ P) followed by self-etching adhesive systems with MDPB monomer (Clearfil Protect Bond/ PB) and without (Clearfil SE Bond/ Cl) on Streptococcus mutans (Sm) and Lactobacillus casei (Lc). Study design: The dentin of twenty human teeth was exposed to prepare four cylindrical cavities in each tooth. The cavities were incubated with Sm or Lc. One cavity from each tooth served as contamination control (positive control group); the other three were treated with P, P+CL and P+PB. The cavities were sealed and after 72 h, dentin samples were collected and microbial cultivation was performed. Microbial count was undertaken (CFUs/mg) according to the morphological characteristics for Sm and Lc. Analysis of variance and Tukey's test were applied (alpha=5%). Results: For Sm, groups P+Cl and P+PB had lower microbial count than group P (no statistical differences between P+Cl and P+PB). For Lc, group P+Cl had microbial count similar to group P There was statistical difference between cavities treated with P and P+PB but none between groups P+Cl and P+PB. After using P, both self-etching adhesives showed antimicrobial potential, although Clearfil Protect Bond proved better against Lc. Conclusions: Both self-etching adhesives used after application of Papacarie showed antimicrobial potential, although Clearfil Protect Bond proved more effective against Lactobacillus casei. (AU)

FAPESP's process: 09/14737-0 - Chemical agent containing papain on antimicrobial potential of cariogenic microrganisms followed by the use of self-etching adhesive systems and antimicrobial activity of self-etching adhesive systems as a function of time and light sources
Grantee:Roberta Tarkany Basting Höfling
Support Opportunities: Regular Research Grants