Characterization of the renal renin-angiotensin system in transgenic mice that express rat tonin

Autor(es)

Ribeiro, Amanda A. ^[1] ; Palomino, Zaira ^[1] ; Lima, Mercia P. ^[2] ; Souza, Leandro E. ^[3] ; Ferreira, Daniele S. ^[4] ; Pesquero, Joao B. ^[4] ; Irigoyen, Maria C. ^[3] ; Pesquero, Jorge L. ^[5] ; Casarini, Dulce E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Div Nephrol, Dept Med, Escola Paulista Med, Sao Paulo - Brazil

^[2] Univ Fed Minas Gerais, Sch Nursing, Dept Basic Nursing, Belo Horizonte, MG - Brazil

^[3] Univ Sao Paulo, Sch Med, Inst Heart, BR-05508 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Biophys, Sao Paulo - Brazil

^[5] Univ Fed Minas Gerais, Dept Biophys, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF THE RENIN-ANGIOTENSIN-ALDOSTERONE SYSTEM; v. 16, n. 4, p. 947-955, DEC 2015.
• Citações Web of Science: 3

Resumo

Introduction: Tonin is an enzyme that is able to generate angiotensin II (Ang II) from angiotensin I (Ang I) or directly from angiotensinogen. Our goal was to characterize the renal renin-angiotensin system in transgenic mice that express rat tonin (TGM`(rTon)). Materials and methods: Mice were euthanized and the kidneys removed for analysis. Tonin activity was evaluated by radioimmunoassay and angiotensin I-converting enzyme (ACE) activity by HPLC. Tonin, ACE and angiotensin II-converting enzyme (ACE2) expression was analyzed by Western blotting. Results: Tonin activity was significantly increased in TGM`(rTon) compared to their respective wild-type (WT) littermates (1.7 0.21 vs 0.11 +/- 0.02 nmol of Ang II/min/mg of protein). Tonin activity had a strong positive correlation with tonin expression in both TGM`(rTon) and their respective wild-type littermates. The ACE activity and expression levels of 65-kDa N-domain angiotensin I-converting enzyme isoform were significantly increased in the TGM`(rTon) when compared with WT. ACE2 expression levels were statistically significantly higher in the TGM`(rTon) when compared with WT. Angiotensin 1-7 (Ang(1-7)) and Ang I levels were significantly lower in the TGM`(rTon). Conclusions: We suggest that the environment of tonin abundance may increase N-domain ACE activity liberated by a secretase able to cleave somatic ACE. (AU)

• Processo FAPESP: 09/03261-4 - Caracterização do sistema renina angiotensina local no rim e coração em camundongo transgênico que expressa tonina de rato.
• Beneficiário: Amanda Aparecida Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Mestrado