Busca avançada
Ano de início
Entree
(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Overexpression of mitochondrial uncoupling protein 1 (UCP1) induces a hypoxic response in Nicotiana tabacum leaves

Texto completo
Autor(es):
Barreto, Pedro [1] ; Okura, Vagner [1] ; Pena, Izabella A. [1] ; Maia, Renato [1] ; Maia, Ivan G. [2] ; Arruda, Paulo [1, 3]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083875 Campinas, SP - Brazil
[2] UNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP - Brazil
[3] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Genet & Evolucao, BR-13083875 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Journal of Experimental Botany; v. 67, n. 1, p. 301-313, JAN 2016.
Citações Web of Science: 4
Resumo

Mitochondrial uncoupling protein 1 (UCP1) decreases reactive oxygen species production under stress conditions by uncoupling the electrochemical gradient from ATP synthesis. This study combined transcriptome profiling with experimentally induced hypoxia to mechanistically dissect the impact of Arabidopsis thaliana UCP1 (AtUCP1) overexpression in tobacco. Transcriptomic analysis of AtUCP1-overexpressing (P07) and wild-type (WT) plants was carried out using RNA sequencing. Metabolite and carbohydrate profiling of hypoxia-treated plants was performed using H-1-nuclear magnetic resonance spectroscopy and high-performance anion-exchange chromatography with pulsed amperometric detection. The transcriptome of P07 plants revealed a broad induction of stress-responsive genes that were not strictly related to the mitochondrial antioxidant machinery, suggesting that overexpression of AtUCP1 imposes a strong stress response within the cell. In addition, transcripts that mapped into carbon fixation and energy expenditure pathways were broadly altered. It was found that metabolite markers of hypoxic adaptation, such as alanine and tricarboxylic acid intermediates, accumulated in P07 plants under control conditions at similar rates to WT plants under hypoxia. These findings indicate that constitutive overexpression of AtUCP1 induces a hypoxic response. The metabolites that accumulated in P07 plants are believed to be important in signalling for an improvement in carbon assimilation and induction of a hypoxic response. Under these conditions, mitochondrial ATP production is less necessary and fermentative glycolysis becomes critical to meet cell energy demands. In this scenario, the more flexible energy metabolism along with an intrinsically activated hypoxic response make these plants better adapted to face several biotic and abiotic stresses. (AU)

Processo FAPESP: 14/17634-5 - A mitocôndria como elemento central na resposta a estresses em plantas
Beneficiário:Pedro Paulo Augusto Fabiano Arantes Pereira Barreto
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 12/00235-5 - Mecanismos de indução da via da sacaropina em células humanas
Beneficiário:Izabella Agostinho Pena
Linha de fomento: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 14/24183-0 - Impacto da superexpressão da proteína desacopladora mitocondrial 1 (UCP1) no metabolismo fotossintético
Beneficiário:Renato Maia Souza Filho
Linha de fomento: Bolsas no Brasil - Iniciação Científica