Expression of mRNA Encoding the LH Receptor (LHR) and LHR Binding Protein in Granulosa Cells from Nelore (Bos indicus) Heifers Around Follicle Deviation

Autor(es)

Ereno, R. L. ^[1] ; Loureiro, B. ^[2] ; Castilho, A. C. S. ^[1] ; Machado, M. F. ^[1] ; Pegorer, M. F. ^[1] ; Satrapa, R. A. ^[1] ; Nogueira, M. F. G. ^[3] ; Buratini, J. ^[4] ; Barros, C. M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo State, Dept Pharmacol, Inst Biosci, BR-18610307 Botucatu, SP - Brazil

^[2] Univ Vila Velha, Lab Anim Reprod Physiol, Vila Velha, ES - Brazil

^[3] Univ Sao Paulo State, Dept Biol Sci, Assis, SP - Brazil

^[4] Univ Sao Paulo State, Dept Physiol, Inst Biosci, BR-18610307 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: REPRODUCTION IN DOMESTIC ANIMALS; v. 50, n. 6, p. 952-957, DEC 2015.
• Citações Web of Science: 2

Resumo

The time at which follicles acquire LHR in bovine granulosa cells is the subject of some controversy among researchers. The main objective of the present study was to assess the mRNA expression of LHR and LRBP (mRNA protein binding), a post-transcriptional suppressor of LHR mRNA expression, in granulosa cells from the two largest follicles around the expected time of follicle deviation in Nelore heifers. First, the interval between ovulation and follicle deviation in 20 Nelore heifers was determined (2.3 +/- 0.2 days after ovulation). Ovulation was hormonally synchronized, and then, heifers were slaughtered on days 2, 2.5 and 3 after ovulation (before, during and after, respectively, the expected time of follicle deviation), and granulosa cells from the two largest follicles were collected. The mRNA abundance of an LHR fragment common to all isoforms (total LHR) and LRBP was assessed by real-time RT-PCR, and LHR alternative transcripts were assessed by semiquantitative RT-PCR followed by electrophoresis. LHR mRNA expression was not detected before the expected time of deviation. Total LHR mRNA abundance was greater in the largest follicle and increased from day 2.5 to 3. In contrast, LRBP mRNA was detected starting on day 2 and was more expressed in the second largest follicle on days 2.5 and 3. The present data suggest that the expression of LHR mRNA in bovine granulosa cells is established after follicle deviation and that the lower abundance of LRBP mRNA after the expected time of deviation may contribute to greater expression of LHR in the bovine dominant follicle. (AU)

• Processo FAPESP: 10/52250-2 - Identificacao, caracterizacao e estudos in vitro dos mecanismos de sinalizacao celular dos transcritos alternativos do receptor de lh (lhr) na especie bovina.
• Beneficiário: Anthony César de Souza Castilho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 10/51789-5 - Expressao de genes associados a qualidade, desenvolvimento e competencia oocitaria e embrionaria inicial de femeas nelores submetidas ou nao a super estimulacao ovariana
• Beneficiário: Rafael Augusto Satrapa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/10737-8 - Comparação entre a maturação in vitro convencional e um sistema de simulação da maturação oocitária fisiológica (spom) em complexos cumulus-oócito bovinos
• Beneficiário: Mariana Fernandes Machado
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/50593-2 - Estudos de genes associados à qualidade, competência oocitária e embrionária inicial de fêmeas Nelores e Angus submetidas ou não à superestimulação ovariana e dos mecanismos envolvidos na sinalização intracelular das diferentes isoformas de LHR na espécie bovina
• Beneficiário: Ciro Moraes Barros
• Modalidade de apoio: Auxílio à Pesquisa - Regular