Label-Free Quantitative Proteomic Analysis of Puccinia psidii Uredospores Reveals Differences of Fungal Populations Infecting Eucalyptus and Guava

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Quecine, Maria Carolina ^[1] ; Leite, Thiago Falda ^[1] ; Bini, Andressa Peres ^[1] ; Regiani, Thais ^[1] ; Franceschini, Livia Maria ^[1] ; Frasson Budzinski, Ilara Gabriela ^[1] ; Marques, Felipe Garbelini ^[1] ; Veneziano Labate, Monica Teresa ^[1] ; Guidetti-Gonzalez, Simone ^[1] ; Moon, David Henry ^[1] ; Labate, Carlos Alberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 11, n. 1 JAN 5 2016.
• Citações Web of Science: 4

Resumo

Puccinia psidii sensu lato (s.l.) is the causal agent of eucalyptus and guava rust, but it also attacks a wide range of plant species from the myrtle family, resulting in a significant genetic and physiological variability among populations accessed from different hosts. The uredo-spores are crucial to P. psidii dissemination in the field. Although they are important for the fungal pathogenesis, their molecular characterization has been poorly studied. In this work, we report the first in-depth proteomic analysis of P. psidii s.l. uredospores from two contrasting populations: guava fruits (PpGuava) and eucalyptus leaves (PpEucalyptus). NanoUPLC-MSE was used to generate peptide spectra that were matched to the UniProt Puccinia genera sequences (UniProt database) resulting in the first proteomic analysis of the phytopathogenic fungus P. psidii. Three hundred and fourty proteins were detected and quantified using Label free proteomics. A significant number of unique proteins were found for each sample, others were significantly more or less abundant, according to the fungal populations. In PpGuava population, many proteins correlated with fungal virulence, such as malate dehydrogenase, proteossomes subunits, enolases and others were increased. On the other hand, PpEucalyptus proteins involved in biogenesis, protein folding and translocation were increased, supporting the physiological variability of the fungal populations according to their protein reservoirs and specific host interaction strategies. (AU)

• Processo FAPESP: 08/50361-1 - Genômica funcional aplicada à descoberta de genes de resistência à ferrugem do eucalipto
• Beneficiário: Carlos Alberto Labate
• Linha de fomento: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE
• Processo FAPESP: 11/17455-5 - Análise de metabólitos e proteínas totais em folhas de Eucalyptus grandis durante a infecção por Puccinia psidii
• Beneficiário: Felipe Garbelini Marques
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/50445-0 - Estado da variabilidade genética de Puccinia psidiiwinter agente causal de ferrugem em Eucalyptus spp
• Beneficiário: Maria Carolina Quecine Verdi
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/22227-4 - Análise global dos mecanismos moleculares envolvidos na tolerância a seca e acúmulo de sacarose em duas variedades de cana-de-açúcar
• Beneficiário: Ilara Gabriela Frasson Budzinski
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/07596-6 - Estudo molecular do fitopatógeno Puccinia psidii Winter, agente causal da ferrugem, in vitro e durante sua interação com Eucalyptus spp
• Beneficiário: Andressa Peres Bini
• Linha de fomento: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 10/15417-6 - Caracterização do proteoma e fosfoproteoma de cana-de-açúcar sob estresse hídrico
• Beneficiário: Simone Guidetti Gonzalez
• Linha de fomento: Bolsas no Brasil - Pós-Doutorado